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Investigating The Changes In Maternal Serum Brain-Derived Neurotrophic Factor Levels In Patients Undergoing Spontaneous Vaginal Delivery

Year 2015, Volume: 46 Issue: 1, 6 - 12, 26.02.2015
https://doi.org/10.16948/zktb.47653

Abstract

Objective: To reveal the changes in maternal serum brain-derived neurotrophic factor (BDNF) and malondialdehyde (MDA) levels in women undergoing spontaneous vaginal delivery.

Material and Method: This prospective study was done in sixty-two patients with term pregnancy undergoing spontaneous vaginal delivery. Maternal blood samples in the latent phase of labor (T1), immediately before clamping the umbilical cord (T2) and 24 hours after birth (T3); and umbilical artery cord blood sample immediately after umbilical cord clamping at delivery were obtained. MDA and BDNF levels in collected blood samples were measured and results were compared using appropriate statistical tests. The relationship between cord blood levels of BDNF with cord blood MDA and maternal serum levels of BDNF with maternal serum MDA was investigated.

Results: Maternal serum BDNF levels (pg/ml) were significantly higher in the blood sample collected at the T3 time point (3729.92 ± 621.68) compared to blood samples collected at T1 (956.75 ± 291.47) and T2 (985.50 ±291.91)time points (P<0.0001, for both). Cord blood BDNF levels (834.91 ± 235.73)were significantly lower compared to maternal blood samples collected at T1, T2 and T3 time points (P=0.01, P=0.002, P<0.0001; retrospectively). Maternal serum MDA levels (µmol/ml) at T3 time point (6.42 ± 1.70) were found to be lower compared with those of collected blood samples at T1 (8.43 ± 2.67) and T2 (8.80 ± 3.11) time points (P<0.0001, for both). Also, cord blood MDA levels (9.98 ± 1.70) were higher compared to maternal serum MDA levels collected at T1, T2 and T3 time points (P=0.001, for T2 and P<0.0001, for T1 and T3 time points). A negative association was found between both maternal and cord blood BDNF levels with maternal and cord blood MDA levels, respectively (r= -0.374, P<0.0001; r= -0.310, P=0.014; respectively).

Conclusion:   Changes in maternal BDNF and MDA levels occur in women undergoing spontaneous vaginal delivery. These changes may be associated with increased maternal oxidative stress during labor.

References

  • (1) Huang EJ, Reichardt LF. Neurotrophins: roles in neuronal development and function. Annu Rev Neurosci 2001;24:677-736
  • (2) Chao MV, Rajagopal R, Lee FS. Neurotrophin signalling in health and disease. Clin Sci (Lond) 2006;110(2):167-173.
  • (3) Chouthai NS, Sampers J, Desai N, Smith GM. Changes in neurotrophin levels in umbilical cord blood from infants with different gestational ages and clinical conditions. Pediatr Res 2003;53(6):965–969.
  • (4) Karege F, Schwald M, Cisse M. Postnatal developmental profile of brain derived neurotrophic factor in rat brain and platelets. Neurosci Lett 2002;328(3):261–264.
  • (5) Kenna HA, Reynolds-May M, Stepanenko A, Ketter TA, Hallmayer J, Rasgon NL. Blood levels of brain derived neurotrophic factor in women with bipolar disorder and healthy control women. J Affect Disord 2014;156:214-218.
  • (6) O'Keefe LM, Doran SJ, Mwilambwe-Tshilobo L, Conti LH, Venna VR, McCullough LD. Social isolation after stroke leads to depressive-like behavior and decreased BDNF levels in mice. Behav Brain Res 2014;260:162-170.
  • (7) Koo JW, Park CH, Choi SH, Kim NJ, Kim HS, Choe JC, Suh YH. The postnatal environment can counteract prenatal effects on cognitive ability, cell proliferation, and synaptic protein expression. FASEB J 2003;17(11):1556-1558.
  • (8) Girotti AW. Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res 1998;39(8):1529-1542.
  • (9) Stanzani L, Zoia C, Sala G, Appollonio I, Frattola L, De Simoni MG, Ferrarese C. Nerve growth factor and transforming growth factor-beta serum levels in acute stroke patients. Possible involvement of neurotrophins in cerebrovascular disease. Cerebrovasc Dis 2001;12(3):240-244.
  • (10) Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351–358.
  • (11) Nakahashi T, Fujimura H, Altar CA, Li J, Kambayashi J, Tandon NN, Sun B. Vascular endothelial cells synthesize and secrete brain-derived neurotrophic factor. FEBS Lett 2000;470(2):113-117.
  • (12) Kodomari I, Wada E, Nakamura S, Wada K. Maternal supply of BDNF to mouse fetal brain through the placenta. Neurochem Int 2009;54(2):95-98.
  • (13) Fan CG, Zhang QJ, Tang FW, Han ZB, Wang GS, Han ZC. Human umbilical cord blood cells express neurotrophic factors. Neurosci Lett 2005;380(3):322-325.
  • (14) Miller FD, Kaplan DR. Neurotrophin signalling pathways regulating neuronal apoptosis. Cell Mol Life Sci 2001;58(8):1045-1053.
  • (15) Ferrer I, Krupinski J, Goutan E, Martí E, Ambrosio S, Arenas E. Brain-derived neurotrophic factor reduces cortical cell death by ischemia after middle cerebral artery occlusion in the rat. Acta Neuropathol 2001;101(3):229-238.
  • (16) Brownridge P. The nature and consequences of childbirth pain. Eur J Obstet Gynecol Reprod Biol 1995;59:9-15.
  • (17) Hicks RR, Martin VB, Zhang L, Seroogy KB. Mild experimental brain injury differentially alters the expression of neurotrophin and neurotrophin receptor mRNAs in the hippocampus. Exp Neurol 1999;160(2):469-478.
  • (18) Marshall JS, Gomi K, Blennerhassett MG, Bienenstock J. Nerve growth factor modifies the expression of inflammatory cytokines by mast cells via a prostanoid-dependent mechanism. J Immunol 1999;162(7):4271-4276.
  • (19) Kapczinski F, Frey BN, Andreazza AC, Kauer-Sant'Anna M, Cunha AB, Post RM. Increased oxidative stress as a mechanism for decreased BDNF levels in acute manic episodes. Rev Bras Psiquiatr 2008;30(3):243-245.
  • (20) Rouhe H, Salmela-Aro K, Halmesmäki E, Saisto T. Fear of childbirth according to parity, gestational age, and obstetric history. BJOG 2009;116(1):67-73.
  • (21) Alipour Z, Lamyian M, Hajizadeh E, Vafaei MA. The association between antenatal anxiety and fear of childbirth in nulliparous women: a prospective study. Iran J Nurs Midwifery Res 2011;16(2):169-73.
  • (22) Vakilian K, Ranjbar A, Zarganjfard A, Mortazavi M, Vosough-Ghanbari S, Mashaiee S, Abdollahi M. On the relation of oxidative stress in delivery mode in pregnant women; a toxicological concern. Toxicol Mech Methods 2009;19(2):94-99.
  • (23) Dermitzaki E, Staikou C, Petropoulos G, Rizos D, Siafaka I, Fassoulaki A. A randomized study of maternal serum cytokine levels following cesarean section under general or neuraxial anesthesia. Int J Obstet Anesth 2009;18(1):33-37.
  • (24) Ben-Jonathan N, LaPensee CR, LaPensee EW: What can we learn from rodents about prolactin in humans? Endocr Rev 2008;29(1):1–41.
  • (25) Tabata H, Kobayashi M, Ikeda JH, Nakao N, Saito TR, Tanaka M: Characterization of multiple first exons in murine prolactin receptor gene and the effect of prolactin on their expression in the choroid plexus. J Mol Endocrinol 2012;48(2),169–176.
  • (26) Morales T. Recent findings on neuroprotection against excitotoxicity in the hippocampus of female rats. J Neuroendocrinol 2011;23(11):994–1001.
  • (27) Donner N, Bredewold R, Maloumby R, Neumann ID. Chronic intracerebral prolactin attenuates neuronal stress circuitries in virgin rats. Eur J Neurosci 2007;25(6):1804–1814.
  • (28) Malamitsi-Puchner A, Economou E, Rigopoulou O, Boutsikou T. Perinatal changes of brain-derived neurotrophic factor in pre- and fullterm neonates. Early Hum Dev 2004;76(1):17-22.
  • (29) Dhobale M, Mehendale S, Pisal H, Nimbargi V, Joshi S. Reduced maternal and cord nerve growth factor levels in preterm deliveries. Int J Dev Neurosci 2012;30(2):99-103.

Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması

Year 2015, Volume: 46 Issue: 1, 6 - 12, 26.02.2015
https://doi.org/10.16948/zktb.47653

Abstract

Amaç: Spontan vajinal doğum eylemindeki kadınlarda maternal serum beyin kaynaklı nörotrofik faktör (BDNF) ve malondialdehit (MDA) seviyelerinde oluşan değişiklikleri ortaya koymak.

Gereç ve Yöntem: Bu prospektif çalışma, spontan vaginal doğum eylemindeki term gebeliği olan 62 hastada yapıldı. Doğumun latent fazında (T1), göbek kordonu klemplenmeden hemen önce (T2) ve doğumdan 24 saat sonra (T3) maternal kan örnekleri; doğumda göbek kordonu klemplendikten hemen sonra umblikal arterden kord kanı örneği alındı. Alınan kan örneklerindeki MDA ve BDNF seviyeleri ölçüldü ve uygun istatistik testler kullanılarak sonuçlar karşılaştırıldı. Kord kanı BDNF ile kord kanı MDA seviyeleri ve maternal serum BDNF ile maternal serum MDA seviyeleri arasındaki ilişki araştırıldı.

Bulgular: T3 zamanındaki maternal serum BDNF seviyeleri (pg/ml) (3729,92 ± 621,68) T1 (956,75 ± 291,47) ve T2 (985,50 ± 291,91)’ den yüksek bulundu (P<0,0001, her ikisi için). Kord kanı BDNF seviyeleri (834,91 ± 235,73)ise T1, T2 ve T3 zamanlarında alınan maternal serum BDNF seviyelerinden daha düşüktü(P=0,01; P=0,002, P<0,0001; geriye dönük olarak). T3 zamanındaki maternal serum MDA değerleri (µmol/ml) (6,42 ± 1,70), T1 (8,43 ± 2,67) ve T2 (8,80 ± 3,11) zamanlarında değerlerden daha düşük bulundu (P<0,0001; her ikisi için).  Kord kanı MDA seviyeleri (9,98 ± 1,70)ise T1, T2 ve T3 zamanlarında alınan maternal serum MDA seviyelerinegöre yüksekti (P=0,001; T2 için ve P<0,0001; T1 ve T3 için). Maternal serum MDA ile BDNF seviyeleri arasında ve kord kanı MDA ile BDNF düzeyleri arasında negatif korelasyon vardı (r= -0,374; P<0,0001; r= -0,310; P=0,014; geriye dönük olarak).

Sonuç: Spontan vajinal doğum yapan kadınlarda maternal BDNF ve MDA düzeylerinde değişiklikler meydana gelir.  Bu değişiklikler, doğum eylemi sırasında artan maternal oksidatif stres ile ilişkili olabilir. 

References

  • (1) Huang EJ, Reichardt LF. Neurotrophins: roles in neuronal development and function. Annu Rev Neurosci 2001;24:677-736
  • (2) Chao MV, Rajagopal R, Lee FS. Neurotrophin signalling in health and disease. Clin Sci (Lond) 2006;110(2):167-173.
  • (3) Chouthai NS, Sampers J, Desai N, Smith GM. Changes in neurotrophin levels in umbilical cord blood from infants with different gestational ages and clinical conditions. Pediatr Res 2003;53(6):965–969.
  • (4) Karege F, Schwald M, Cisse M. Postnatal developmental profile of brain derived neurotrophic factor in rat brain and platelets. Neurosci Lett 2002;328(3):261–264.
  • (5) Kenna HA, Reynolds-May M, Stepanenko A, Ketter TA, Hallmayer J, Rasgon NL. Blood levels of brain derived neurotrophic factor in women with bipolar disorder and healthy control women. J Affect Disord 2014;156:214-218.
  • (6) O'Keefe LM, Doran SJ, Mwilambwe-Tshilobo L, Conti LH, Venna VR, McCullough LD. Social isolation after stroke leads to depressive-like behavior and decreased BDNF levels in mice. Behav Brain Res 2014;260:162-170.
  • (7) Koo JW, Park CH, Choi SH, Kim NJ, Kim HS, Choe JC, Suh YH. The postnatal environment can counteract prenatal effects on cognitive ability, cell proliferation, and synaptic protein expression. FASEB J 2003;17(11):1556-1558.
  • (8) Girotti AW. Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res 1998;39(8):1529-1542.
  • (9) Stanzani L, Zoia C, Sala G, Appollonio I, Frattola L, De Simoni MG, Ferrarese C. Nerve growth factor and transforming growth factor-beta serum levels in acute stroke patients. Possible involvement of neurotrophins in cerebrovascular disease. Cerebrovasc Dis 2001;12(3):240-244.
  • (10) Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351–358.
  • (11) Nakahashi T, Fujimura H, Altar CA, Li J, Kambayashi J, Tandon NN, Sun B. Vascular endothelial cells synthesize and secrete brain-derived neurotrophic factor. FEBS Lett 2000;470(2):113-117.
  • (12) Kodomari I, Wada E, Nakamura S, Wada K. Maternal supply of BDNF to mouse fetal brain through the placenta. Neurochem Int 2009;54(2):95-98.
  • (13) Fan CG, Zhang QJ, Tang FW, Han ZB, Wang GS, Han ZC. Human umbilical cord blood cells express neurotrophic factors. Neurosci Lett 2005;380(3):322-325.
  • (14) Miller FD, Kaplan DR. Neurotrophin signalling pathways regulating neuronal apoptosis. Cell Mol Life Sci 2001;58(8):1045-1053.
  • (15) Ferrer I, Krupinski J, Goutan E, Martí E, Ambrosio S, Arenas E. Brain-derived neurotrophic factor reduces cortical cell death by ischemia after middle cerebral artery occlusion in the rat. Acta Neuropathol 2001;101(3):229-238.
  • (16) Brownridge P. The nature and consequences of childbirth pain. Eur J Obstet Gynecol Reprod Biol 1995;59:9-15.
  • (17) Hicks RR, Martin VB, Zhang L, Seroogy KB. Mild experimental brain injury differentially alters the expression of neurotrophin and neurotrophin receptor mRNAs in the hippocampus. Exp Neurol 1999;160(2):469-478.
  • (18) Marshall JS, Gomi K, Blennerhassett MG, Bienenstock J. Nerve growth factor modifies the expression of inflammatory cytokines by mast cells via a prostanoid-dependent mechanism. J Immunol 1999;162(7):4271-4276.
  • (19) Kapczinski F, Frey BN, Andreazza AC, Kauer-Sant'Anna M, Cunha AB, Post RM. Increased oxidative stress as a mechanism for decreased BDNF levels in acute manic episodes. Rev Bras Psiquiatr 2008;30(3):243-245.
  • (20) Rouhe H, Salmela-Aro K, Halmesmäki E, Saisto T. Fear of childbirth according to parity, gestational age, and obstetric history. BJOG 2009;116(1):67-73.
  • (21) Alipour Z, Lamyian M, Hajizadeh E, Vafaei MA. The association between antenatal anxiety and fear of childbirth in nulliparous women: a prospective study. Iran J Nurs Midwifery Res 2011;16(2):169-73.
  • (22) Vakilian K, Ranjbar A, Zarganjfard A, Mortazavi M, Vosough-Ghanbari S, Mashaiee S, Abdollahi M. On the relation of oxidative stress in delivery mode in pregnant women; a toxicological concern. Toxicol Mech Methods 2009;19(2):94-99.
  • (23) Dermitzaki E, Staikou C, Petropoulos G, Rizos D, Siafaka I, Fassoulaki A. A randomized study of maternal serum cytokine levels following cesarean section under general or neuraxial anesthesia. Int J Obstet Anesth 2009;18(1):33-37.
  • (24) Ben-Jonathan N, LaPensee CR, LaPensee EW: What can we learn from rodents about prolactin in humans? Endocr Rev 2008;29(1):1–41.
  • (25) Tabata H, Kobayashi M, Ikeda JH, Nakao N, Saito TR, Tanaka M: Characterization of multiple first exons in murine prolactin receptor gene and the effect of prolactin on their expression in the choroid plexus. J Mol Endocrinol 2012;48(2),169–176.
  • (26) Morales T. Recent findings on neuroprotection against excitotoxicity in the hippocampus of female rats. J Neuroendocrinol 2011;23(11):994–1001.
  • (27) Donner N, Bredewold R, Maloumby R, Neumann ID. Chronic intracerebral prolactin attenuates neuronal stress circuitries in virgin rats. Eur J Neurosci 2007;25(6):1804–1814.
  • (28) Malamitsi-Puchner A, Economou E, Rigopoulou O, Boutsikou T. Perinatal changes of brain-derived neurotrophic factor in pre- and fullterm neonates. Early Hum Dev 2004;76(1):17-22.
  • (29) Dhobale M, Mehendale S, Pisal H, Nimbargi V, Joshi S. Reduced maternal and cord nerve growth factor levels in preterm deliveries. Int J Dev Neurosci 2012;30(2):99-103.
There are 29 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section OBSTETRICS AND GYNECOLOGY
Authors

Ayşe Aksoy

İlay Gözükara

Suna Kucur

Gonca Batmaz This is me

Esra Laloğlu This is me

Elif Bulut This is me

Publication Date February 26, 2015
Published in Issue Year 2015 Volume: 46 Issue: 1

Cite

APA Aksoy, A., Gözükara, İ., Kucur, S., Batmaz, G., et al. (2015). Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması. Zeynep Kamil Tıp Bülteni, 46(1), 6-12. https://doi.org/10.16948/zktb.47653
AMA Aksoy A, Gözükara İ, Kucur S, Batmaz G, Laloğlu E, Bulut E. Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması. Zeynep Kamil Tıp Bülteni. February 2015;46(1):6-12. doi:10.16948/zktb.47653
Chicago Aksoy, Ayşe, İlay Gözükara, Suna Kucur, Gonca Batmaz, Esra Laloğlu, and Elif Bulut. “Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör Ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması”. Zeynep Kamil Tıp Bülteni 46, no. 1 (February 2015): 6-12. https://doi.org/10.16948/zktb.47653.
EndNote Aksoy A, Gözükara İ, Kucur S, Batmaz G, Laloğlu E, Bulut E (February 1, 2015) Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması. Zeynep Kamil Tıp Bülteni 46 1 6–12.
IEEE A. Aksoy, İ. Gözükara, S. Kucur, G. Batmaz, E. Laloğlu, and E. Bulut, “Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması”, Zeynep Kamil Tıp Bülteni, vol. 46, no. 1, pp. 6–12, 2015, doi: 10.16948/zktb.47653.
ISNAD Aksoy, Ayşe et al. “Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör Ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması”. Zeynep Kamil Tıp Bülteni 46/1 (February 2015), 6-12. https://doi.org/10.16948/zktb.47653.
JAMA Aksoy A, Gözükara İ, Kucur S, Batmaz G, Laloğlu E, Bulut E. Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması. Zeynep Kamil Tıp Bülteni. 2015;46:6–12.
MLA Aksoy, Ayşe et al. “Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör Ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması”. Zeynep Kamil Tıp Bülteni, vol. 46, no. 1, 2015, pp. 6-12, doi:10.16948/zktb.47653.
Vancouver Aksoy A, Gözükara İ, Kucur S, Batmaz G, Laloğlu E, Bulut E. Spontan Vajinal Doğum Yapan Hastalarda Maternal Serum Beyin Kaynaklı Nörotrofik Faktör ve Malondialdehit Düzeylerindeki Değişimlerin Araştırılması. Zeynep Kamil Tıp Bülteni. 2015;46(1):6-12.