Yüksek fruktoz içerikli beslenmenin overlerde perilipin ekspresyonu ve polikistik over gelişimi ile ilişkisi

Year 2016, Volume: 47 Issue: 4, 106 - 111, 08.11.2016

İlknur Keskin , Nejda Bedri

https://doi.org/10.16948/zktipb.257211

Abstract

Amaç: PCOS ve yüksek fruktoz içerikli diyet
birlikteliğini gösteren çalışmalar kısıtlı sayıdadır. Çalışmamızda ratlarda
yüksek fruktoz içerikli beslenmenin overlerde perilipin ekspresyonu üzerine
etkisi ve Polikistik Over gelişimi ile ilgisini ortaya koymayı amaçladık.

Gereç ve Yöntem: Çalışmamızda hiçbir uygulama
yapılmayan Kontrol (K) grubu, Yüksek Fruktoz (HF) grubu ve DHEA
(dehidroepiandrosteron) enjekte edilen Hormon (H) grubu over yapıları
karşılaştırıldı. Gruplar arasındaki morfolojik farklılıkları gösterebilmek için
hematoksilen-eozin boyaması yapıldı, PLIN2 ve PLIN3 antikorları için western
blot yöntemi uygulandı. Progesteron ve LH seviyeleri ELISA tekniği ile ölçüldü.

Bulgular: HF ve Hormon gruplarında antral ve
preantral folikül sayısının artmış olduğu gözlendi. PLIN2 ve PLIN3
ekspresyonlarının Hormon ve HF gruplarında yüksek olduğu görüldü. Progesteron
ve LH seviyelerinde anlamlı bir farklılık saptanmadı.

Sonuç: Yüksek fruktozlu diyet ile beslenme
PCOS oluşumuna neden olabilir ve bu mekanizmada PLIN2 ve PLIN3 proteinlerinin
aşırı ekspresyonu etkili olabilir.

Keywords

fare, fruktoz, ovaryum, PCOS

Relationship between maintaning diet containing high fructose and development of polycystic ovary via perilipin expression

Abstract

Introduction: Diet comes at the first place
among factors affecting PCOS development. Studies showing diet consisting of
PCOS combined with high fructose are in a limited level. In this study, it is
aimed to investigate PLIN2 and PLIN3 expressions by showing diet consisting of
combination of high fructose and PCOS in Juvenile phase.

Material-Methods: A total of 26 Balb/C
type female mice have been used and 3 groups are created: Control (no
treatment), HF (tap water containing 20% fructose is given by oral gavege) and Hormone
(The injection of DHEA  is injected s.c. for
20 days). In order to show the morphological differences between the groups haematoxylin and eosin staining method has been used. For PLIN2 and PLIN3 antibodies western blot method
has been used. ELISA
technic has been used to compare progesterone and LH levels between the groups.

Results: It is seen that antral and preantral follicle numbers of HF and Hormone
groups have increased. It is also seen that PLIN2 and PLIN3 expressions are
high in HF and Hormone groups. As a result diet with high fructose could
contribute to PCOS development and in this mechanism PLIN2 and PLIN3 proteins
could also be effective. 

Keywords

mice, fructose, ovary, PCOS

References

• 1- Ehrmann DA. Polycystic ovary syndrome. N Engl J Med 2005;352(12):1223-36.
• 2- Melo AS, Vieira CS, Barbieri MA, Rosa-E-Silva AC, Silva AA, Cardoso VC et al. High prevalence of polycystic ovary syndrome in women born small for gestational age. Hum Reprod 2010;25(8):2124-31.
• 3- Norman RJ, Dewailly D, Legro RS, Hickey TE. Polycystic ovary syndrome. Lancet 2007;370(9588):685-97.
• 4- Vos MB, Kimmons JE, Gillespie C, Welsh J, Blanck HM. Dietary fructose consumption among US children and adults: the Third National Health and Nutrition Examination Survey. Medscape J Med 2008;10(7):160
• 5- Storey ML, Forshee RA, Anderson PA. Beverage consumption in the US population. J Am Diet Assoc 2006:106(12):1992-2000.
• 6- Feinman RD, Fine EJ. Fructose in perspective. Nutr Metab (Lond) 2013;10(1):45.
• 7- Parks EJ, Skokan LE, Timlin MT, Dingfelder CS. Dietary sugars stimulate fatty acid synthesis in adults. J Nutr 2008;138(6):1039-46.
• 8- Lecoultre V, Egli L, Carrel G, Theytaz F, Kreis R, Schneiter P et al. Effects of fructose and glucose overfeeding on hepatic insulin sensitivity and intrahepatic lipids in healthy humans. Obesity (Silver Spring) 2013;21(4):782-5.
• 9- Dunaif A. Insulin resistance and the polycystic ovary syndrome: mechanism and implications for pathogenesis. Endocr Rev 1997;18(6):774-800.
• 10- Franks S, Gilling-Smith C, Watson H, Willis D. Insulin action in the normal and polycystic ovary. Endocrinol Metab Clin North Am 1999;28(2):361-78.
• 11- Franks S, Mason H, Willis D. Follicular dynamics in the polycystic ovary syndrome. Mol Cell Endocrinol 2000;163(1-2):49-52.
• 12- Morin-Papunen LC, Vauhkonen I, Koivunen RM, Ruokonen A, Tapanainen JS. Insulin sensitivity, insulin secretion, and metabolic and hormonal parameters in healthy women and women with polycystic ovarian syndrome. Hum Reprod 2000;15(6):1266-74.
• 13- Pasquali R, Gambineri A. Metabolic effects of obesity on reproduction. Reprod Biomed Online 2006;12(5):542-51.
• 14- Dumesic DA, Damario MA, Session DR, Famuyide A, Lesnick TG, Thornhill ARet al. Ovarian morphology and serum hormone markers as predictors of ovarian follicle recruitment by gonadotropins for in vitro fertilization. J Clin Endocrinol Metab 2001:86(6):2538-43.
• 15- Jonard S, Robert Y, Cortet-Rudelli C, Pigny P, Decanter C, Dewailly D. Ultrasound examination of polycystic ovaries: is it worth counting the follicles? Hum Reprod 2003;18(3):598-603.
• 16- Wang H, Bell M, Sreenivasan U, Hu H, Liu J, Dalen K et al. Unique regulation of adipose triglyceride lipase (ATGL) by perilipin 5, a lipid droplet-associated protein. J Biol Chem 2011;286(18):15707-15.
• 17- Murphy DJ. The biogenesis and functions of lipid bodies in animals, plants and microorganisms. Prog Lipid Res 2001;40(5):325-438.
• 18- Thiele C, Spandl J. Cell biology of lipid droplets. Curr Opin Cell Biol 2008;20(4):378-85.
• 19- Walther TC, Farese RV Jr. The life of lipid droplets. Biochim Biophys Acta 2009;1791(6): 459-66.
• 20- Holm C. Molecular mechanisms regulating hormone-sensitive lipase and lipolysis. Biochem Soc Trans 2003;31(6):1120-4.
• 21- Khor VK, Shen WJ, Kraemer FB. Lipid droplet metabolism. Curr Opin Clin Nutr Metab Care 2013;16(6):632-7.
• 22- Bickel PE, Tansey JT, Welte MA. PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores. Biochim Biophys Acta 2009;1791(6):419-40.
• 23- Liu X, Xue R, Ji L, Zhang X, Wu J, Gu J et al. Activation of farnesoid X receptor (FXR) protects against fructose-induced liver steatosis via inflammatory inhibition and ADRP reduction. Biochem Biophys Res Commun 2014;450(1):117-23.
• 24- Covington JD, Bajpeyi S, Moro C, Tchoukalova YD, Ebenezer PJ, Burk DH et all. Potential effects of aerobic exercise on the expression of perilipin 3 in the adipose tissue of women with polycystic ovary syndrome: a pilot study. Eur J Endocrinol 2015;172(1):47-58.
• 25- Sastre D, da Costa NN, de Sá AL, Conceição SD, Chiaratti MR, Adona PR et al. Expression of PLIN2 and PLIN3 during oocyte maturation and early embryo development in cattle. Theriogenology 2014;81(2):326-31.
• 26- Yang X, Dunning KR, Wu LL, Hickey TE, Norman RJ, Russell DL et al. Identification of Perilipin-2 as a lipid droplet protein regulated in oocytes during maturation. Reprod Fertil Dev 2010;22(8):1262-71.
• 27- Zhang RN, Fu XW, Jia BY, Liu C, Cheng KR, Zhu SE. Expression of Perilipin 2 (PLIN2) in Porcine Oocytes During Maturation. Reprod Domest Anim 2014;49(5):875-80.
• 28- Cardiff RD, Miller CH, Munn RJ. Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb Protoc 2014;6:655-8.
• 29- Shen WJ, Patel S, Miyoshi H, Greenberg AS, Kraemer FB. Functional interaction of hormone-sensitive lipase and perilipin in lipolysis. J Lipid Res 2009;50(11):2306-13.
• 30- Yan W, Chen S, Huang J, Shen Y, Qiang B, Gu D. Polymorphisms in PLIN and hypertension combined with obesity and lipid profiles in Han Chinese. Obes Res 2004;12:1733-7.
• 31- Manneras-Holm L, Leonhardt H, Kullberg J, Jennische E, Oden A, Holm G, et all. Adipose tissue has aberrant morphology and function in PCOS: enlarged adipocytes and low serum adiponectin, but not circulating sex steroids, are strongly associated with insulin resistance. J Clin Endocrinol Metab 2011;96(2):304-11.