Research Article
BibTex RIS Cite

Erkek infertilitesinde seminal leptin seviyelerinin semen parametreleri ve DNA fragmantasyonu ile ilişkisi

Year 2017, Volume: 48 Issue: 2, 39 - 43, 15.02.2017
https://doi.org/10.16948/zktipb.288940

Abstract

Giriş:
Bu çalışmamızda insan semen leptin düzeylerini ve semen parametreleri ile
ilişkisinin incelenmesi amaçlanmıştır.



Materyal-Metod:
Normal ve oligoastenoteratospermik 30 hastalık gruplar oluşturuldu. Hastaların
semen örneklerinde leptin ve DNA fragmantasyon düzeyleri incelendi, ayrıca
sperm sayısını ve motilite oranlarını içeren semen parametreleri değerlendirildi.



Bulgular:
İnfertil hastalarda sperm sayısı ve motilite oranları normal gruba göre anlamlı
olarak azalmıştır: 6,40±4,29 (
x106/mL), % 38,63±14,92 (p<0,0001). DNA
fragmantasyon oranları normal grupta % 21,00±8,23 olarak gözlenirken infertil
erkeklerde % 38,43±14,23  oranıyla
anlamlı olarak yüksek bulunmuş, semen leptin oraları ise normal grupta
347,58±111,13
ng/mL olarak  gözlenirken, infertil grupta 267,63±56,36 ng/mL düzeyi ile anlamlı
olarak azaldığı gözlenmiştir. İnfertil gruplar kendi içinde incelendiği zaman
ise semen leptin düzeyi ile yaş, volüm, sperm sayısı ve motilitesi arasında
istatistiksel olarak anlamlı olacak bir ilişki saptanmamıştır.



Sonuç:
Çalışmamızda, oligoastenoteratozoospermik erkek infertilitesinde serum leptin
seviyelerinin azalmış olduğunu, ama sperm motilitesi ile anlamlı bir ilişkinin
bulunmadı gözlemlenmiştir. Leptinin semende tanısal bir kriter olarak
kullanılabilmesi için daha geniş kapsamlı çalışmaların yapılması gerektiği
düşünülmektedir

References

  • 1. Jianhua Guo, Yang Zhao, Weiying Huang, Wei Hu, Jianjun Gu, Chuhong Chen, Juan Zhou, Yubing Peng, Min Gong, Zhong Wang., Sperm motility inversely correlates with seminal leptin levels in idiopathic asthenozoospermia. Int J Clin Exp Med 2014;7(10):3550-3555.
  • 2. Sigman M, Howards SS: Male infertility: Campbell’s Urology. (Walsh PC, Retik AB, Vaughan ED, Wein AJ, ed). Seventh edition. Philadelphia, Saunders company. 1998:1287-1330.
  • 3. Sokol RZ: Endocrinology and male infertility. Infertility and Reproductive Medicine Clinics of North America. (Werthman PE, ed). Philadelphia, Saunders. 1999:427-434.
  • 4. Homan GF, Davies M and Norman R. The impact of lifestyle factors on reproductive performance in the general population and those undergoing infertility treatment: a review. Hum Reprod Update 2007; 13: 209-223.
  • 5. Fernandez CD, Bellentani FF, Fernandes GS, Perobelli JE, Favareto AP, Nascimento AF, Cicogna AC and Kempinas WD. Diet-induced obesity in rats leads to a decrease in sperm motility. Reprod Biol Endocrinol 2011; 9: 32.
  • 6. Rosenbaum M, Leibel RL: The role of leptin in human physiology. N Engl J Med,1999; 341: 913-915.
  • 7. Jacobs HS, Seibell MM: Polycystic Ovary Syndrome. Infertility. (Seibell MM, ed). Second edition. Appleton,1997; 121-133.
  • 8. Andò S and Aquila S. Arguments raised by the recent discovery that insulin and leptin are expressed in and secreted by human ejaculated spermatozoa. Mol Cell Endocrinol 2005; 245: 1-6.
  • 9. Farooqi IS, Jebb SA, Langmack G, et al: Effects of re-combinant leptin therapy in a child with congenital leptin deficiency. N Engl J Med, 1999; 341: 879-884.
  • 10. Nazian SJ, Cameron DF: Temporal relation between leptin and various indices of sexual maturation in the male rat. J Androl, 1999; 20: 487-491.
  • 11. Wang J, Liu R, Hawkins M, Barzilai N, Rossetti L:Anutrient-sensing pathway regulates leptin gene expression in muscle and fat. Nature 1998; 18: 684-688.
  • 12. Bado A, Levasseur S, Attoub S, Kermorgant S, Laigneau JP, Bortoluzzi MN, et al; The stomach is a source of leptin. Nature 1998; 20: 790-793.
  • 13. Hoggard N, Hunter L, Duncan JS, Williams LM, Trayhurn P, Mercer JG. Leptin and leptin receptor mRNA and protein expression in the murine fetus and placenta. Proc Natl Acad Sci 1997; 94: 11073- 8.
  • 14. Karlsson C, Lindell K, Svensson E, Bergh C, Lind P, Billig H, Carlsson LMS, Carlsson B .Expression of functional leptin receptors in the human ovary. J Clin Endocrinol Metab 1997;82: 4144-8.
  • 15. Chehab FF, Mounzih K, Lu R, Lim ME. Early onset of reproductive function in normal female mice treated with leptin. Science 1997;275: 88-90.
  • 16. Barash IA, Cheung CC, Weigle DS, et al. Leptin is a metabolic signal to the reproductive system. Endocrinology, 1996;137: 3144-3147.
  • 17. Baskin DG, Hahn TM, Schwartz MW. Leptin sensitive neurons in the hypothalamus. Horm Metab Res 1999;31: 345-350.
  • 18. Haron MN, D'Souza UJ, Jaafar H, Zakaria R, Jeet Singh H. Exogenous leptin administration decreases sperm count and increases the fraction of abnormal sperm in adult rats. Fertil Steril 2009; 93: 322-324.
  • 19. Aquila S, Gentile M, Middea M, Catalano S, Morelli C, Pezzi V, et al. Leptin secretion by human ejaculated spermatozoa. J Clin Endocrinol Metab 2005; 90: 4753–4761.
  • 20. Li HW, Chiu PC, Cheung MP, Yeung WS, O WS. Effect of leptin on motility, capacitation and acrosome reaction of human spermatozoa. Int J Androl 2008; 32: 687- 694.
  • 21. Glander HJ, Lammert A, Paasch U, Glasow A, Kratzsch J. Lepin exits in tubuli seminiferi and in seminal plasma. Andrologia 2002; 34:227-233.
  • 22. Moschos S, Chan JL, Mantzoros CS. Leptin and reproduction: a review. Fertil Steril 2002; 3: 433-444.
  • 23. Caprioa M, Fabbrinic E, Riccid G, Bascianic S, Gnessic L, Arizzic M, et al. Ontogenesis of leptin receptor in rat leydig cells. Biol Reprod 2003; 68: 1199-1207.
  • 24. Lange Consiglio A, Dell'Aquila ME, Fiandanese N, Ambruosi B, Cho YS, Bosi G, et al. Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. Reprod Biol Endocrinol 2009; 7:113.
  • 25. Smith GD, Jackson LM, Foster DL. Leptin regulation of reproductive function and fertility. Theriogenology 2002; 57: 73-86.
  • 26. Steinman N, Gamzu R, Yogev L, et al: Serum leptin concentrations are higher in azoospermic than in normozoospermic men. Fertil Steril 2001;75: 821-822, 2001.
  • 27. Tunç L, Tokgöz H, Kordan Y, Biri H, Karaoğlan Ü, Bozkırlı İ. Oligospermik erkek infertilitesinde Leptin’in yeri. Türk Üroloji Dergisi 2005;31(2):207-211.
  • 28. Jorsaraei A, Shibahara H, Ayustawati, Hirano Y, Suzuki T, Marzony ET, Zainalzadeh M, Suzuki M. The Leptin concentrations in seminal plasma of men and its relationship to semen parameters. Iranian Journal of Reproductive Medicine 2010; 8:3. 95-100.
  • 29. Caprio M, Fabbrini E, Isiodori AM, Aversa A, Fabbri A. Leptin in reproduction. Trends Endocrinol Metab 2001; 12: 65-72.
  • 30. Camina JP, Lage M, Menendez C, Grana M, Garcia-Devesa J, Dieguez C, et al. Evidence of free leptin in human seminal plasma. Endocrine 2002; 17: 169-174.

The relationship of seminal leptin levels with semen parameters and DNA fragmentation

Year 2017, Volume: 48 Issue: 2, 39 - 43, 15.02.2017
https://doi.org/10.16948/zktipb.288940

Abstract

Objective:
The objective of
this study is to investigate the relationship between human seminal leptin
levels and semen parameters.

Material
& Methods:
Study
groups include 30 males with oligoasthenoteratozoospermia and 30 males who have
normal semen parameters. Leptin and DNA fragmentation levels were investigated
in both groups, along with a variety of semen parameters including sperm count
and motility.

Results:
In comparison with the normal males,
sperm count and motility have decreased significantly in infertile patients: 6,40±4,29
(x106/mL), %, 38,63±14,92 (p<0,0001).  DNA fragmentation levels were found as
21,00±8,23 %   and
38,43±14,23 %,  in normal and patient
groups respectively. Seminal leptin levels were 347,58±111,13
ng/mL in the normal group, while there was a significant
decrease to 267,63±56,36
ng/mL in the infertile group. Among infertile patient
groups, no significant difference was observed regarding the relationship
between seminal leptin levels and age, volume, sperm count and motility.







Conclusion: In our study it was demonstrated that, serum leptin
levels decrease in oligoasthenoteratozoospermic infertile males, while there is
not a significant relation with the motility of sperm. In order to use seminal
leptin levels as a diagnostic tool more extensive studies are needed.

References

  • 1. Jianhua Guo, Yang Zhao, Weiying Huang, Wei Hu, Jianjun Gu, Chuhong Chen, Juan Zhou, Yubing Peng, Min Gong, Zhong Wang., Sperm motility inversely correlates with seminal leptin levels in idiopathic asthenozoospermia. Int J Clin Exp Med 2014;7(10):3550-3555.
  • 2. Sigman M, Howards SS: Male infertility: Campbell’s Urology. (Walsh PC, Retik AB, Vaughan ED, Wein AJ, ed). Seventh edition. Philadelphia, Saunders company. 1998:1287-1330.
  • 3. Sokol RZ: Endocrinology and male infertility. Infertility and Reproductive Medicine Clinics of North America. (Werthman PE, ed). Philadelphia, Saunders. 1999:427-434.
  • 4. Homan GF, Davies M and Norman R. The impact of lifestyle factors on reproductive performance in the general population and those undergoing infertility treatment: a review. Hum Reprod Update 2007; 13: 209-223.
  • 5. Fernandez CD, Bellentani FF, Fernandes GS, Perobelli JE, Favareto AP, Nascimento AF, Cicogna AC and Kempinas WD. Diet-induced obesity in rats leads to a decrease in sperm motility. Reprod Biol Endocrinol 2011; 9: 32.
  • 6. Rosenbaum M, Leibel RL: The role of leptin in human physiology. N Engl J Med,1999; 341: 913-915.
  • 7. Jacobs HS, Seibell MM: Polycystic Ovary Syndrome. Infertility. (Seibell MM, ed). Second edition. Appleton,1997; 121-133.
  • 8. Andò S and Aquila S. Arguments raised by the recent discovery that insulin and leptin are expressed in and secreted by human ejaculated spermatozoa. Mol Cell Endocrinol 2005; 245: 1-6.
  • 9. Farooqi IS, Jebb SA, Langmack G, et al: Effects of re-combinant leptin therapy in a child with congenital leptin deficiency. N Engl J Med, 1999; 341: 879-884.
  • 10. Nazian SJ, Cameron DF: Temporal relation between leptin and various indices of sexual maturation in the male rat. J Androl, 1999; 20: 487-491.
  • 11. Wang J, Liu R, Hawkins M, Barzilai N, Rossetti L:Anutrient-sensing pathway regulates leptin gene expression in muscle and fat. Nature 1998; 18: 684-688.
  • 12. Bado A, Levasseur S, Attoub S, Kermorgant S, Laigneau JP, Bortoluzzi MN, et al; The stomach is a source of leptin. Nature 1998; 20: 790-793.
  • 13. Hoggard N, Hunter L, Duncan JS, Williams LM, Trayhurn P, Mercer JG. Leptin and leptin receptor mRNA and protein expression in the murine fetus and placenta. Proc Natl Acad Sci 1997; 94: 11073- 8.
  • 14. Karlsson C, Lindell K, Svensson E, Bergh C, Lind P, Billig H, Carlsson LMS, Carlsson B .Expression of functional leptin receptors in the human ovary. J Clin Endocrinol Metab 1997;82: 4144-8.
  • 15. Chehab FF, Mounzih K, Lu R, Lim ME. Early onset of reproductive function in normal female mice treated with leptin. Science 1997;275: 88-90.
  • 16. Barash IA, Cheung CC, Weigle DS, et al. Leptin is a metabolic signal to the reproductive system. Endocrinology, 1996;137: 3144-3147.
  • 17. Baskin DG, Hahn TM, Schwartz MW. Leptin sensitive neurons in the hypothalamus. Horm Metab Res 1999;31: 345-350.
  • 18. Haron MN, D'Souza UJ, Jaafar H, Zakaria R, Jeet Singh H. Exogenous leptin administration decreases sperm count and increases the fraction of abnormal sperm in adult rats. Fertil Steril 2009; 93: 322-324.
  • 19. Aquila S, Gentile M, Middea M, Catalano S, Morelli C, Pezzi V, et al. Leptin secretion by human ejaculated spermatozoa. J Clin Endocrinol Metab 2005; 90: 4753–4761.
  • 20. Li HW, Chiu PC, Cheung MP, Yeung WS, O WS. Effect of leptin on motility, capacitation and acrosome reaction of human spermatozoa. Int J Androl 2008; 32: 687- 694.
  • 21. Glander HJ, Lammert A, Paasch U, Glasow A, Kratzsch J. Lepin exits in tubuli seminiferi and in seminal plasma. Andrologia 2002; 34:227-233.
  • 22. Moschos S, Chan JL, Mantzoros CS. Leptin and reproduction: a review. Fertil Steril 2002; 3: 433-444.
  • 23. Caprioa M, Fabbrinic E, Riccid G, Bascianic S, Gnessic L, Arizzic M, et al. Ontogenesis of leptin receptor in rat leydig cells. Biol Reprod 2003; 68: 1199-1207.
  • 24. Lange Consiglio A, Dell'Aquila ME, Fiandanese N, Ambruosi B, Cho YS, Bosi G, et al. Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. Reprod Biol Endocrinol 2009; 7:113.
  • 25. Smith GD, Jackson LM, Foster DL. Leptin regulation of reproductive function and fertility. Theriogenology 2002; 57: 73-86.
  • 26. Steinman N, Gamzu R, Yogev L, et al: Serum leptin concentrations are higher in azoospermic than in normozoospermic men. Fertil Steril 2001;75: 821-822, 2001.
  • 27. Tunç L, Tokgöz H, Kordan Y, Biri H, Karaoğlan Ü, Bozkırlı İ. Oligospermik erkek infertilitesinde Leptin’in yeri. Türk Üroloji Dergisi 2005;31(2):207-211.
  • 28. Jorsaraei A, Shibahara H, Ayustawati, Hirano Y, Suzuki T, Marzony ET, Zainalzadeh M, Suzuki M. The Leptin concentrations in seminal plasma of men and its relationship to semen parameters. Iranian Journal of Reproductive Medicine 2010; 8:3. 95-100.
  • 29. Caprio M, Fabbrini E, Isiodori AM, Aversa A, Fabbri A. Leptin in reproduction. Trends Endocrinol Metab 2001; 12: 65-72.
  • 30. Camina JP, Lage M, Menendez C, Grana M, Garcia-Devesa J, Dieguez C, et al. Evidence of free leptin in human seminal plasma. Endocrine 2002; 17: 169-174.
There are 30 citations in total.

Details

Subjects Health Care Administration
Journal Section Original Research
Authors

Şule Ayla

İlknur Keskin This is me

Tuba Varlı Yelke This is me

Tuğçe Önel This is me

Serçin Karahüseyinoğlu This is me

Publication Date February 15, 2017
Published in Issue Year 2017 Volume: 48 Issue: 2

Cite

APA Ayla, Ş., Keskin, İ., Varlı Yelke, T., Önel, T., et al. (2017). Erkek infertilitesinde seminal leptin seviyelerinin semen parametreleri ve DNA fragmantasyonu ile ilişkisi. Zeynep Kamil Tıp Bülteni, 48(2), 39-43. https://doi.org/10.16948/zktipb.288940
AMA Ayla Ş, Keskin İ, Varlı Yelke T, Önel T, Karahüseyinoğlu S. Erkek infertilitesinde seminal leptin seviyelerinin semen parametreleri ve DNA fragmantasyonu ile ilişkisi. Zeynep Kamil Tıp Bülteni. June 2017;48(2):39-43. doi:10.16948/zktipb.288940
Chicago Ayla, Şule, İlknur Keskin, Tuba Varlı Yelke, Tuğçe Önel, and Serçin Karahüseyinoğlu. “Erkek Infertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri Ve DNA Fragmantasyonu Ile ilişkisi”. Zeynep Kamil Tıp Bülteni 48, no. 2 (June 2017): 39-43. https://doi.org/10.16948/zktipb.288940.
EndNote Ayla Ş, Keskin İ, Varlı Yelke T, Önel T, Karahüseyinoğlu S (June 1, 2017) Erkek infertilitesinde seminal leptin seviyelerinin semen parametreleri ve DNA fragmantasyonu ile ilişkisi. Zeynep Kamil Tıp Bülteni 48 2 39–43.
IEEE Ş. Ayla, İ. Keskin, T. Varlı Yelke, T. Önel, and S. Karahüseyinoğlu, “Erkek infertilitesinde seminal leptin seviyelerinin semen parametreleri ve DNA fragmantasyonu ile ilişkisi”, Zeynep Kamil Tıp Bülteni, vol. 48, no. 2, pp. 39–43, 2017, doi: 10.16948/zktipb.288940.
ISNAD Ayla, Şule et al. “Erkek Infertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri Ve DNA Fragmantasyonu Ile ilişkisi”. Zeynep Kamil Tıp Bülteni 48/2 (June 2017), 39-43. https://doi.org/10.16948/zktipb.288940.
JAMA Ayla Ş, Keskin İ, Varlı Yelke T, Önel T, Karahüseyinoğlu S. Erkek infertilitesinde seminal leptin seviyelerinin semen parametreleri ve DNA fragmantasyonu ile ilişkisi. Zeynep Kamil Tıp Bülteni. 2017;48:39–43.
MLA Ayla, Şule et al. “Erkek Infertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri Ve DNA Fragmantasyonu Ile ilişkisi”. Zeynep Kamil Tıp Bülteni, vol. 48, no. 2, 2017, pp. 39-43, doi:10.16948/zktipb.288940.
Vancouver Ayla Ş, Keskin İ, Varlı Yelke T, Önel T, Karahüseyinoğlu S. Erkek infertilitesinde seminal leptin seviyelerinin semen parametreleri ve DNA fragmantasyonu ile ilişkisi. Zeynep Kamil Tıp Bülteni. 2017;48(2):39-43.