Year 2019, Volume 20, Issue 2, Pages 91 - 97 2019-04-29

LEPTIN QUANTITY DECREASES İN TYPE 1 DIABETES MELLITUS INDUCUED RATS BRAIN
TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR

Erhan ŞAHİN [1] , Öykü ÖZCAN [2] , Ezgi BEKTUR [3] , Cengiz BAYÇU [4] , Ümide ÖZKAY DEMİR [5] , Özgür Devrim CAN [6] , Varol ŞAHİNTÜRK [7]

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OBJECTIVE: Leptin hormone plays an important role in regulation of appetite and body metabolism and is mainly synthesized in fat tissue. It is known that leptin is transported to the brain via choroid plexus after synthesized in the fat tissue. The aim of this study is to investigate the amount and expression of leptin in the brain of rats with type 1 diabetes mellitus.

MATERIAL AND METHODS: A total of 14 adult male Wistar Albino rats were divided into 2 equal groups (n = 7). No application was made to the control group. A single dose (55 mg/kg) of streptozotocin was administered intraperitoneally to the animals of the diabetes mellitus group and it was considered that type 1 diabetes mellitus developed when blood glucose level > 280 mg/dL. Brain samples taken at the end of the experiment were fixed with %10 formaldehyde and then subjected to routine tissue monitoring followed by leptin immunohistochemical staining. The amount of leptin was determined by western blotting in brain tissues.

RESULTS: In all groups leptin staining was detected only in the choroid plexus of rat brains. According to this knowlodge, it was determined that leptin staining decreased in type 1 diabetes mellitus developed rats. The decreased leptin in the type 1 diabetes mellitus group was showed by western blot too.

CONCLUSIONS: Type 1 diabetes reduces the expression and amount of leptin in the choroid plexus.

AMAÇ: Leptin hormonu, iştah ve vücut metabolizmasının düzenlenmesinde önemli görevler üstlenmekte ve başlıca yağ dokusunda sentezlenmektedir. Leptinin yağ dokuda sentezlendikten sonra koroid pleksus aracılığı ile beyne taşındığı bilinmektedir. Bu çalışmanın amacı Tip 1 diyabetes mellitus oluşturulan sıçanların beyninde leptin ifadesinin ve miktarının araştırılmasıdır.

GEREÇ VE YÖNTEM: Çalışmamızda toplam 14 adet yetişkin, erkek Wistar Albino sıçan 2 eşit gruba ayrıldı (n=7). Kontrol grubuna hiçbir uygulama yapılmadı. Diyabetes mellitus grubundaki hayvanlara ise tek doz (55 mg/kg) streptozotosin intraperitoneal olarak verildi ve kan glukoz seviyesi >280 mg/dL ölçüldüğünde Tip 1diyabetes mellitus geliştiği kabul edildi. Deney sonunda alınan beyin örnekleri %10’luk formaldehit ile fikse edildikten sonra rutin doku takip işleminin ardından alınan kesitlere leptin immünohistokimyasal boyaması uygulandı. Beyin dokularında western blot yöntemi ile leptin miktarına bakıldı.

BULGULAR: Tüm gruplara ait beyin kesitlerinde sadece koroid pleksusta leptin boyanması saptandı. Buna göre, leptin boyanmasının Tip 1 diyabetes mellitus geliştirilen sıçanlarda azaldığı saptandı. Western blot ile Tip 1 diyabetes mellitus grubunda leptin miktarının belirgin olarak azaldığı saptandı.

SONUÇ: Bu çalışmayla tokluk hissini oluşturmak üzere koroid pleksus üzerinden beyne geçen leptin hormonu ile diyabet arasında yakın bir ilişki olduğu ve bu hormonun diyabetle azaldığı gösterilmiştir. Bu çalışmadan yola çıkarak Tip 1 diyabet ve leptin hormonu ilişkisi derinlemesine irdelenmelidir.

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Primary Language tr
Subjects Medicine
Published Date nisan 2019
Journal Section Articles
Authors

Author: Erhan ŞAHİN
Country: Turkey


Author: Öykü ÖZCAN

Author: Ezgi BEKTUR

Author: Cengiz BAYÇU

Author: Ümide ÖZKAY DEMİR

Author: Özgür Devrim CAN

Author: Varol ŞAHİNTÜRK

Bibtex @research article { kocatepetip408118, journal = {Kocatepe Tıp Dergisi}, issn = {1302-4612}, eissn = {2149-7869}, address = {Afyonkarahisar Sağlık Bilimleri Üniversitesi}, year = {2019}, volume = {20}, pages = {91 - 97}, doi = {10.18229/kocatepetip.408118}, title = {TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR}, key = {cite}, author = {ŞAHİN, Erhan and ÖZCAN, Öykü and BEKTUR, Ezgi and BAYÇU, Cengiz and ÖZKAY DEMİR, Ümide and CAN, Özgür Devrim and ŞAHİNTÜRK, Varol} }
APA ŞAHİN, E , ÖZCAN, Ö , BEKTUR, E , BAYÇU, C , ÖZKAY DEMİR, Ü , CAN, Ö , ŞAHİNTÜRK, V . (2019). TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. Kocatepe Tıp Dergisi, 20 (2), 91-97. DOI: 10.18229/kocatepetip.408118
MLA ŞAHİN, E , ÖZCAN, Ö , BEKTUR, E , BAYÇU, C , ÖZKAY DEMİR, Ü , CAN, Ö , ŞAHİNTÜRK, V . "TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR". Kocatepe Tıp Dergisi 20 (2019): 91-97 <http://dergipark.org.tr/kocatepetip/issue/44892/408118>
Chicago ŞAHİN, E , ÖZCAN, Ö , BEKTUR, E , BAYÇU, C , ÖZKAY DEMİR, Ü , CAN, Ö , ŞAHİNTÜRK, V . "TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR". Kocatepe Tıp Dergisi 20 (2019): 91-97
RIS TY - JOUR T1 - TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR AU - Erhan ŞAHİN , Öykü ÖZCAN , Ezgi BEKTUR , Cengiz BAYÇU , Ümide ÖZKAY DEMİR , Özgür Devrim CAN , Varol ŞAHİNTÜRK Y1 - 2019 PY - 2019 N1 - doi: 10.18229/kocatepetip.408118 DO - 10.18229/kocatepetip.408118 T2 - Kocatepe Tıp Dergisi JF - Journal JO - JOR SP - 91 EP - 97 VL - 20 IS - 2 SN - 1302-4612-2149-7869 M3 - doi: 10.18229/kocatepetip.408118 UR - https://doi.org/10.18229/kocatepetip.408118 Y2 - 2018 ER -
EndNote %0 Kocatepe Medical Journal TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR %A Erhan ŞAHİN , Öykü ÖZCAN , Ezgi BEKTUR , Cengiz BAYÇU , Ümide ÖZKAY DEMİR , Özgür Devrim CAN , Varol ŞAHİNTÜRK %T TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR %D 2019 %J Kocatepe Tıp Dergisi %P 1302-4612-2149-7869 %V 20 %N 2 %R doi: 10.18229/kocatepetip.408118 %U 10.18229/kocatepetip.408118
ISNAD ŞAHİN, Erhan , ÖZCAN, Öykü , BEKTUR, Ezgi , BAYÇU, Cengiz , ÖZKAY DEMİR, Ümide , CAN, Özgür Devrim , ŞAHİNTÜRK, Varol . "TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR". Kocatepe Tıp Dergisi 20 / 2 (April 2019): 91-97. https://doi.org/10.18229/kocatepetip.408118