Year 2018, Volume 8, Issue 2, Pages 17 - 24 2018-12-01

Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu

Necibe Kayak [1] , Önder Türkmen [2] , Ali Tevfik Uncu [3] , Yeşim Dal [4]

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This study was carried out to determine the molecular characterization of 83 well-established lines in the prospective S4 stage in the edible pumpkins (Cucurbita pepo L.). The molecular diversity among the pumpkins genotypes was determined by SSR markers. Thirty one EST- SSR markers yielded a total of 122 amplified DNA fragments, of which 107 (87.70%) were polymorphic, were used to measure the degree of genetic diversity. The DARwin software was used to generate a Dice coefficient dissimilarity matrix that was then used to construct an unweighted neighbor-joining dendrogram of the accessions. Neighbor-joining dendrogram demonstrated that the germplasm collection is divided into three main groups. The highest PIC value was calculated for the marker CUTC006209 as 0.42. According to the Mantel test results, correlation of the dissimilarity matrix and the dendrogram was 0.99 which is a very high score that indicates a very strong degree of correlation. Genetic relationships calculated based on molecular genetic data demonstrated the degree of diversity within the population along with the pattern of clustering among accessions which will provide valuable information to breeders for future breeding programs.
Edible pupkins, molecular description, SSR
  • TÜİK, 2014. 26.03.2017. https://biruni.tuik.gov.tr/bitkiselapp/bitkisel.zul.
  • Blanca, J., Canizares, J., Roig, C., Ziarsolo, P., Nuez, F. ve Pico, B., 2011, Transcriptome characterization and high throughput SSRs and SNPs discovery in Cucurbita pepo (Cucurbitaceae), Blanca et al. BMC Genomics, 12:104.
  • Can, H., Türkmen, Ö., Işık, R., Paksoy, M., Seymen, M., Fİdan, S. ve Hakkı, E. E., 2014, Çerezlik Kabak Hatlarının Saflıklarının Dominant Markör Sistemleriyle Belirlenmesi, Uluslararası katılımlı 5. Tohumculuk Kongresi Diyarbakır.
  • Devran, Z., 2003, Moleküler İşaretleyicilerin (Markörlerin) Dayanıklılık Islahında Kullanılması, 20, 1-6.
  • Ding, Q., Li, J., Wang, F., Zhang, Y., Li, H., Zhang, J. ve Gao Jianwei., 2015, Characterization and Development of EST-SSRs by
  • (Brassica rapa L. ssp. pekinensis), Hindawi Publishing Corporation International Journal of Genomics, 2015.
  • Doyle, J. J. ve Doyle, J. L., 1987, A rapid DNA isolation procedure for small quantities of fresh leaf tissue, Phytochemical Bulletin, 19, 11-15.
  • Gong, L., Paris, H. S., Stift, G., Lelley, T., Nee, M. H., Pachner, M. ve Vollmann, J., 2012, Genetic relationships and evolution in Cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms, Theoretical and Applied Genetics, 124, 875-891.
  • OH, F., 1972, The Significance, Utilization and Conservation of Crop Genetic Resources, FAO, Rome.
  • Rahim, B., PeyvastGholamali, Ali, A. M., Babak, R., Akbar, E. A. ve Ali, B., 2013, Biochemical systematic, population structure and genetic variability studies among Iranian Cucurbita (Cucurbita pepo L.) accessions, using genomic SSRs and implications for their breeding potential, Biochemical Systematics and Ecology, 50, 187-198.
  • Robinson RW, Munger HM, Whitaker TW ve GW., B., 1976, Genes of the cucurbitaceae, Hortscience, 11, 554-568.
  • Seymen, M., Türkmen, Ö. ve Paksoy, M., 2012, Selection of edible pumpkin seeds (Cucurbita pepo L.) genotypes, Journal of Selçuk University Natural and Applied Science, 2(4), 29-39.
  • Seymen, M., Yavuz, D., Yavuz, N. ve Türkmen, Ö., 2016, Effect on Yield and Yield Components of Different Irrigation Levels in Edible Seed Pumpkin Growing, world academy of science engineering and technology, 10, 214-219.
  • Solmaz, İ., 2010 Karakterizasyonu Ve Fusarıum Solgunluğu (Fusarium Oxysporum F.Sp. Niveum)’na Dayanımlarının Klasik Ve Moleküler Yöntemlerle Araştırılması, Çukurova Üniversitesi, Adana.
Journal Section Articles
Authors

Author: Necibe Kayak

Author: Önder Türkmen

Author: Ali Tevfik Uncu

Author: Yeşim Dal

Bibtex @ { mjavl521032, journal = {Manas Journal of Agriculture Veterinary and Life Sciences}, issn = {1694-7932}, eissn = {1694-7932}, address = {Kyrgyz-Turkish Manas University}, year = {2018}, volume = {8}, pages = {17 - 24}, doi = {}, title = {Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu}, key = {cite}, author = {Kayak, Necibe and Türkmen, Önder and Uncu, Ali Tevfik and Dal, Yeşim} }
APA Kayak, N , Türkmen, Ö , Uncu, A , Dal, Y . (2018). Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu. Manas Journal of Agriculture Veterinary and Life Sciences, 8 (2), 17-24. Retrieved from http://dergipark.org.tr/mjavl/issue/43034/521032
MLA Kayak, N , Türkmen, Ö , Uncu, A , Dal, Y . "Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu". Manas Journal of Agriculture Veterinary and Life Sciences 8 (2018): 17-24 <http://dergipark.org.tr/mjavl/issue/43034/521032>
Chicago Kayak, N , Türkmen, Ö , Uncu, A , Dal, Y . "Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu". Manas Journal of Agriculture Veterinary and Life Sciences 8 (2018): 17-24
RIS TY - JOUR T1 - Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu AU - Necibe Kayak , Önder Türkmen , Ali Tevfik Uncu , Yeşim Dal Y1 - 2018 PY - 2018 N1 - DO - T2 - Manas Journal of Agriculture Veterinary and Life Sciences JF - Journal JO - JOR SP - 17 EP - 24 VL - 8 IS - 2 SN - 1694-7932-1694-7932 M3 - UR - Y2 - 2019 ER -
EndNote %0 Manas Journal of Agriculture Veterinary and Life Sciences Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu %A Necibe Kayak , Önder Türkmen , Ali Tevfik Uncu , Yeşim Dal %T Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu %D 2018 %J Manas Journal of Agriculture Veterinary and Life Sciences %P 1694-7932-1694-7932 %V 8 %N 2 %R %U
ISNAD Kayak, Necibe , Türkmen, Önder , Uncu, Ali Tevfik , Dal, Yeşim . "Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu". Manas Journal of Agriculture Veterinary and Life Sciences 8 / 2 (December 2018): 17-24.