Year 2019, Volume 6, Issue 1, Pages 25 - 33 2019-03-31

ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE

Ahmad Ali [1] , Dinesh Kumar [2]

16 24

Objectives: The consequences of Diabetes are manifested due to the accumulation of glucose. The carbonyl group of sugars reacts with the amino group of proteins leading to generation of harmful products collectively known as advanced glycation end products (AGEs). These products have been shown to be involved in the various secondary complications of Diabetes and neurodegenerative disorders. The present study involves the assessment of role of Thymoquinone in the process of glycation.

Methods: The in vitro glycation system consisted of BSA and glucose and incubated in the presence and absence of thymoquinone for four weeks at 37 ºC. The amount of glycation products were measured by standard methods like browning, total AGEs by spectrofluorimetry. The aggregation of protein was checked by aggregation index and Congo red assays.  The effect of thymoquinone was also checked on the glycation of DNA and the sample was analyzed by agarose gel electrophoresis.

Results: The presence of thymoquinone resulted in the decrease in browning and amount of total AGEs significantly. There was also a drastic decrease in the glycation-induced aggregation of BSA and reversal of glycoxidative damage of DNA in the presence of thymoquinone.

Conclusion: It can be concluded from these results that thymoquinone is potential antiglycating agent and it can be used to prevent the glycation-induced damage of biomolecules.

Advanced glycation end-products (AGEs), Antiglycation, Browning, Aggregation, DNA damage
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Primary Language en
Journal Section Articles
Authors

Orcid: 0000-0003-4467-5387
Author: Ahmad Ali (Primary Author)
Institution: University of Mumbai
Country: India


Author: Dinesh Kumar
Institution: University of Mumbai
Country: India


Dates

Publication Date: March 31, 2019

Bibtex @research article { nveo528760, journal = {Natural Volatiles and Essential Oils}, issn = {}, eissn = {2148-9637}, address = {Badebio Biotechnology Ltd.}, year = {2019}, volume = {6}, pages = {25 - 33}, doi = {}, title = {ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE}, key = {cite}, author = {Ali, Ahmad and Kumar, Dinesh} }
APA Ali, A , Kumar, D . (2019). ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE. Natural Volatiles and Essential Oils, 6 (1), 25-33. Retrieved from http://dergipark.org.tr/nveo/issue/46268/528760
MLA Ali, A , Kumar, D . "ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE". Natural Volatiles and Essential Oils 6 (2019): 25-33 <http://dergipark.org.tr/nveo/issue/46268/528760>
Chicago Ali, A , Kumar, D . "ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE". Natural Volatiles and Essential Oils 6 (2019): 25-33
RIS TY - JOUR T1 - ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE AU - Ahmad Ali , Dinesh Kumar Y1 - 2019 PY - 2019 N1 - DO - T2 - Natural Volatiles and Essential Oils JF - Journal JO - JOR SP - 25 EP - 33 VL - 6 IS - 1 SN - -2148-9637 M3 - UR - Y2 - 2019 ER -
EndNote %0 Natural Volatiles and Essential Oils ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE %A Ahmad Ali , Dinesh Kumar %T ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE %D 2019 %J Natural Volatiles and Essential Oils %P -2148-9637 %V 6 %N 1 %R %U
ISNAD Ali, Ahmad , Kumar, Dinesh . "ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE". Natural Volatiles and Essential Oils 6 / 1 (March 2019): 25-33.
AMA Ali A , Kumar D . ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE. Nat. Volatiles & Essent. Oils. 2019; 6(1): 25-33.
Vancouver Ali A , Kumar D . ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE. Natural Volatiles and Essential Oils. 2019; 6(1): 33-25.