ANTIGLYCATION AND ANTIAGGREGATION POTENTIAL OF THYMOQUININE

Yıl 2019, Cilt: 6 Sayı: 1, 25 - 33, 31.03.2019

Ahmad Ali , Dinesh Kumar

Öz

Objectives:
The consequences of Diabetes are manifested due to the accumulation of glucose.
The carbonyl group of sugars reacts with the amino group of proteins leading to
generation of harmful products collectively known as advanced glycation end
products (AGEs). These products have been shown to be involved in the various secondary
complications of Diabetes and neurodegenerative disorders. The present study
involves the assessment of role of Thymoquinone in the process of glycation.

Methods:
The in vitro glycation system
consisted of BSA and glucose and incubated in the presence and absence of
thymoquinone for four weeks at 37 ºC. The amount of glycation products were
measured by standard methods like browning, total AGEs by spectrofluorimetry. The
aggregation of protein was checked by aggregation index and Congo red assays.  The effect of thymoquinone was also checked
on the glycation of DNA and the sample was analyzed by agarose gel
electrophoresis.

Results:
The presence of thymoquinone resulted in the decrease in browning and amount of
total AGEs significantly. There was also a drastic decrease in the
glycation-induced aggregation of BSA and reversal of glycoxidative damage of
DNA in the presence of thymoquinone.

Conclusion:
It can be concluded from these results that thymoquinone is potential
antiglycating agent and it can be used to prevent the glycation-induced damage of
biomolecules.

Anahtar Kelimeler

Advanced glycation end-products (AGEs), Antiglycation, Browning, Aggregation, DNA damage

Kaynakça

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