Araştırma Makalesi
TİYENİL-TİYAZOL-ARİL-TİYOÜRE HİBRİT MOLEKÜLLERİN SENTEZİ VE BİYOLOJİK AKTİVİTELERİNİN APOPTOTİK YOL ÜZERİNDEN ANTİ-KANSER ETKİLERİNİN ARAŞTIRILMASI
Öz
Tiyenil-tiyazol-aril-tiyoüre
türevleri (4a-4e) amino-4-(2-tiyenil)tiyazol ve fenilizotiyasiyanatların
reaksiyonları sonucu sentezlenmiştir. Sentezlenen bileşikler IR, 1H-NMR
ve kütle spektrumları ile karekterize edildi. Beş tiyoüre türevinin
anti-proliferatif etkileri A549 (KHDAK) hücrelerinde gerçek zamanlı hücre
analizörü xCELLigence sistemi ile incelendi. Gerçek zamanlı sistemde sitotoksik
olduğu gösterilmiş olan 2 tiyoürenin moleküler etki mekanizmaları,
mitokondriyal membran potansiyeli ΔψM spektrofotometrik olarak, sitokrom c
western blot methoduyla ve Bcl-2 ise qRT-PCR ile araştırılmıştır. 4e
diğerlerinden daha etkili bulunmuştur. 4e
IC50 değerleri 24 ve 48 saat için sırasıyla 29,6 µM ve 22,3 µM
olarak hesaplanmıştır. Bir triflorometil (-CF3) grubunu içeren 4e
molekülü bir anti-kanserojen madde olarak düşünülebilir ve daha ileri
araştırmalar yapılabilir.0000-0003-1761-188X
Anahtar Kelimeler
Kaynakça
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Toplam 34 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Eczacılık ve İlaç Bilimleri |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 20 Eylül 2019 |
| Gönderilme Tarihi | 6 Aralık 2018 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 28 Sayı: 2 |