Oleanolik asidin çekal ligasyon ve punksiyon ile sepsis oluşturulan ratlarda kalp hasarı üzerine etkileri
Öz
Bu çalışmada zeytin ağacı (Olea europeae) ağacının yaprağından saflaştırılan oleanolik asidin (OEA) ratlarda çekal ligasyon ve punksiyonla (CLP) indüklenen sepisisin oluşturduğu kalp hasarı üzerine etkileri incelendi. Her gruba eşit olacak şekilde 30 adet Wistar erkek rat sham, sepsis, OEA-150, OEA-300 ve CEFT olarak 5 gruba ayrıldı. Kromatografik metotlarla zeytin yaprağından saflaştırılan oleanolik asit OEA gruplarına 150 ve 300 mg/kg dozlarda oral yolla uygulandı. CEFT grubuna seftriakson 150 mg/kg dozda intraperitoneal yolla uygulandı. Uygulamadan 24 saat sonra ratlardan kan serumu ve kalp dokuları, histopatolojik, immünohistokimyasal ve biyokimyasal analizler için alındı. Yapılan histopatolojik ve immünohistokimyasal incelemede, sepsis grubunun doku tümör nekrozis faktör (TNF)- ve interlökin (IL)-1β ekspresyonlarında artış, myokartta kas liflerinde şiddetli düzeyde hyalin dejenerasyon, Zenker nekrozu ve damarlarda şiddetli düzeyde hiperemi gözlendi. OEA ve seftriakson (CEFT) sitokin ekspresyonlarında ve histopatolojik bulgularda azalma sağladı. Sepsis, doku superoksit dismutaz aktivitesini (SOD) azalttı. Ayrıca lipid peroksidasyonu (LPO) ve glutatyon (GSH) seviyeleri ile doku katalaz (KAT), serum kreatin kinaz (CK-MB) ve serum laktat dehidrojenaz (LDH) aktivitelerini önemli şekilde arttırdı. OEA ve CEFT, SOD aktivitesini önemli derecede arttırırken diğer parametrelerde belirgin düşüşe neden oldu. Bu çalışmadan elde edilen bulgular ışığında oleanolik asidin sepsis sırasında oluşan kalp dokusu hasarının azaltılmasında faydalı olabileceği düşünülmektedir.
Anahtar Kelimeler
Kaynakça
- Aebi H. (1984). Catalase in vitro. Methods Enzymol. 105, 121-126.
- Andrades M, Ritter C, Moreira JCF, Dal-Pizzol F. (2005). Oxidative Parameters Differences during Non-Lethal and Lethal Sepsis Development1. J Surg Res. 125, 68-72.
- Baykara N, Akalın H, Arslantaş MK, Hancı V, Çağlayan Ç, Kahveci F, Demirağ K, Baydemir C, Ünal N, Özdemir P, Ulusoy H, Memiş D. (2018). Epidemiology of sepsis in intensive care units in Turkey: a multicenter, point-prevalence study. Crit Care. 22, 93.
- Biolo G, Antonione R, De Cicco M. (2007). Glutathione metabolism in sepsis. Crit Care Med. 35, S591-S595.
- Cadirci E, Altunkaynak BZ, Halici Z, Odabasoglu F, Uyanik MH, Gundogdu C, Suleyman H, Halici M, Albayrak M, Unal B. (2010). α-lipoic acid as a potential target for the treatment of lung injury caused by cecal ligation and puncture-induced sepsis model in rats. Shock. 33, 479-484.
- Chousterman BG, Swirski FK, Weber GF. (2017). Cytokine storm and sepsis disease pathogenesis. Semin Immunopathol. 39, 517-528.
- Coskun AK, Yigiter M, Oral A, Odabasoglu F, Halici Z, Mentes O, Cadirci E, Atalay F, Suleyman H. (2011). The effects of Montelukast on antioxidant enzymes and proinflammatory cytokines on the heart, liver, lungs, and kidneys in a rat model of cecal ligation and puncture–induced sepsis. Sci World J. 11, 1341-1356.
- Dadkhah A, Fatemi F. (2011). Heart and kidney oxidative stress status in septic rats treated with caraway extracts. Pharm Biol. 49, 679-686.
- Drosatos K, Lymperopoulos A, Kennel PJ, Pollak N, Schulze PC, Goldberg IJ. (2015). Pathophysiology of sepsis-related cardiac dysfunction: driven by inflammation, energy mismanagement, or both? Curr Heart Fail Rep. 12, 130-140.
- Erol H, Aydın T, Yıldırım S, Can I, Halıcı M. (2020). The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis. Eurasian Journal of Veterinary Sciences. 36, 221-231.
- Erol N, Saglam L, Saglam YS, Erol HS, Altun S, Aktas MS, Halici MB. (2019). The Protection Potential of Antioxidant Vitamins Against Acute Respiratory Distress Syndrome: a Rat Trial. Inflammation. 42, 1585-1594.
- Goyal SN, Mahajan UB, Chandrayan G, Kumawat VS, Kamble S, Patil P, Agrawal YO, Patil CR, Ojha S. (2016). Protective effect of oleanolic acid on oxidative injury and cellular abnormalities in doxorubicin induced cardiac toxicity in rats. Am J Transl Res. 8, 60-69.
- Huang J, Wang Y, Li C, Wang X, He X. (2016). Anti-inflammatory oleanolic triterpenes from Chinese Acorns. Molecules. 21, 669.
- Hwang Y-J, Song J, Kim HR, Hwang KA. (2014). Oleanolic acid regulates NF-κB signaling by suppressing MafK expression in RAW 264.7 cells. BMB reports. 47, 524-529.
- Kısa A, Akyüz M, Çoğun HY, Kordali Ş, Bozhüyük AU, Tezel B, Şiltelioğlu U, Anıl B, Çakır A. (2018). Effects of Olea europaea L. leaf metabolites on the tilapia (Oreochromis niloticus) and three stored pests, Sitophilus granarius, Tribolium confusum and Acanthoscelides obtectus. Rec Nat Prod. 12, 201-215.
- Lee H, Lee IS, Choue R. (2013). Obesity, inflammation and diet. Pediatr Gastroenterol Hepatol Nutr. 16, 143-152.
- Liu J. (1995). Pharmacology of oleanolic acid and ursolic acid. J Ethnopharmacol. 49, 57-68.
- Liu J. (2005). Oleanolic acid and ursolic acid: research perspectives. J Ethnopharmacol. 100, 92-94.
- Marquez-Martin A, De La Puerta R, Fernandez-Arche A, Ruiz-Gutierrez V, Yaqoob P. (2006). Modulation of cytokine secretion by pentacyclic triterpenes from olive pomace oil in human mononuclear cells. Cytokine. 36, 211-217.
- Ohkawa H, Ohishi N, Yagi K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95, 351-358.
- Ozer EK, Goktas MT, Kilinc I, Pehlivan S, Bariskaner H, Ugurluoglu C, Iskit AB. (2017). Coenzyme Q10 improves the survival, mesenteric perfusion, organs and vessel functions in septic rats. Biomed Pharmacother. 91, 912-919.
- Petronilho F, Florentino D, Danielski LG, Vieira LC, Martins MM, Vieira A, Bonfante S, Goldim MP, Vuolo F. (2016). Alpha-lipoic acid attenuates oxidative damage in organs after sepsis. Inflammation. 39, 357-365.
- Reisman SA, Aleksunes LM, Klaassen CD. (2009). Oleanolic acid activates Nrf2 and protects from acetaminophen hepatotoxicity via Nrf2-dependent and Nrf2-independent processes. Biochem Pharmacol. 77, 1273-1282.
- Rocha M, Herance R, Rovira S, Hernández-Mijares A, Victor VM. (2012). Mitochondrial dysfunction and antioxidant therapy in sepsis. Infect Disord Drug Targets. 12, 161-78.
- Sedlak J, Lindsay RH. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 25, 192-205.
- Senthil S, Sridevi M, Pugalendi K. (2007). Cardioprotective effect of oleanolic acid on isoproterenol-induced myocardial ischemia in rats. Toxicol Pathol. 35, 418-423.
- Simmons J, Pittet JF. (2015). The coagulopathy of acute sepsis. Curr Opin Anaesthesiol. 28, 227-236.
- Singh GB, Singh S, Bani S, Gupta BD, Banerjee SK. (1992). Anti-inflammatory activity of oleanolic acid in rats and mice. J Pharm Pharmacol. 44, 456-458.
- Sun Y, Oberley LW, Li Y. (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem. 34, 497-500.
- Topçu G, Öztürk M, Kuşman T, Demirkoz AAB, Kolak U, Ulubelen A. (2013). Terpenoids, essential oil composition, fatty acid profile, and biological activities of Anatolian Salvia fruticosa Mill. Turk J Chem. 37, 619-632.
- Winters BD, Eberlein M, Leung J, Needham DM, Pronovost PJ, Sevransky JE. (2010). Long-term mortality and quality of life in sepsis: a systematic review. Crit Care Med. 38, 1276-1283.
- Yun N, Lee CH, Lee SM. (2009). Protective effect of Aloe vera on polymicrobial sepsis in mice. Food Chem Toxicol. 47, 1341-1348.
- Zhang J, Bi J, Liu S, Pang Q, Zhang R, Wang S, Liu C. (2017). 5-HT Drives Mortality in Sepsis Induced by Cecal Ligation and Puncture in Mice. Mediators Inflamm. 2017, 1-12.
- Zhou L, Gao M, Xiao Z, Zhang J, Li X, Wang A. (2015). Protective effect of astaxanthin against multiple organ injury in a rat model of sepsis. J Surg Res. 195, 559-567.
Öz
In the present, the effects of oleanolic acid (OEA) purified from leaf of the olive tree (Olea europeae) on heart damage caused by cecal ligation and puncture (CLP) induced sepsis model in rats were examined. Thirty Wistar rats equally were divided into six groups as sham, sepsis, OEA-150, OEA-300 and CEFT. Oleanolic acid purified from olive leaf by chromatographic methods was orally administered at doses of 150 and 300 mg/kg to OEA groups. Ceftriaxone was intraperitoneally administered at a dose of 150 mg/kg to the CEFT group. Twenty-four hours after the application, blood serum and heart tissues were taken from rats for histopathological, immunohistochemical and biochemical analysis. In histopathological and immunohistochemical examination, an increase in tissue tumor necrosis factor (TNF)- and interleukin (IL)-1β expressions of the sepsis group, severe hyaline degeneration in muscle fibers in the myocardium, Zenker necrosis and severe hyperemia in the vessels were observed. OEA and ceftriaxone (CEFT) provided a decrease in cytokine expressions and histopathological findings. Sepsis reduced tissue superoxide dismutase activity (SOD). It also significantly increased lipid peroxidation (LPO), glutathione (GSH) levels, catalase (CAT), serum creatine kinase (CK-MB) and serum lactate dehydrogenase (LDH) activities. OEA and CEFT significantly increased SOD activity while causing a significant decrease in other parameters. In the light of the findings from this study, it is thought that oleanolic acid may be beneficial in reducing heart tissue damage during sepsis.
Anahtar Kelimeler
Kaynakça
- Aebi H. (1984). Catalase in vitro. Methods Enzymol. 105, 121-126.
- Andrades M, Ritter C, Moreira JCF, Dal-Pizzol F. (2005). Oxidative Parameters Differences during Non-Lethal and Lethal Sepsis Development1. J Surg Res. 125, 68-72.
- Baykara N, Akalın H, Arslantaş MK, Hancı V, Çağlayan Ç, Kahveci F, Demirağ K, Baydemir C, Ünal N, Özdemir P, Ulusoy H, Memiş D. (2018). Epidemiology of sepsis in intensive care units in Turkey: a multicenter, point-prevalence study. Crit Care. 22, 93.
- Biolo G, Antonione R, De Cicco M. (2007). Glutathione metabolism in sepsis. Crit Care Med. 35, S591-S595.
- Cadirci E, Altunkaynak BZ, Halici Z, Odabasoglu F, Uyanik MH, Gundogdu C, Suleyman H, Halici M, Albayrak M, Unal B. (2010). α-lipoic acid as a potential target for the treatment of lung injury caused by cecal ligation and puncture-induced sepsis model in rats. Shock. 33, 479-484.
- Chousterman BG, Swirski FK, Weber GF. (2017). Cytokine storm and sepsis disease pathogenesis. Semin Immunopathol. 39, 517-528.
- Coskun AK, Yigiter M, Oral A, Odabasoglu F, Halici Z, Mentes O, Cadirci E, Atalay F, Suleyman H. (2011). The effects of Montelukast on antioxidant enzymes and proinflammatory cytokines on the heart, liver, lungs, and kidneys in a rat model of cecal ligation and puncture–induced sepsis. Sci World J. 11, 1341-1356.
- Dadkhah A, Fatemi F. (2011). Heart and kidney oxidative stress status in septic rats treated with caraway extracts. Pharm Biol. 49, 679-686.
- Drosatos K, Lymperopoulos A, Kennel PJ, Pollak N, Schulze PC, Goldberg IJ. (2015). Pathophysiology of sepsis-related cardiac dysfunction: driven by inflammation, energy mismanagement, or both? Curr Heart Fail Rep. 12, 130-140.
- Erol H, Aydın T, Yıldırım S, Can I, Halıcı M. (2020). The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis. Eurasian Journal of Veterinary Sciences. 36, 221-231.
- Erol N, Saglam L, Saglam YS, Erol HS, Altun S, Aktas MS, Halici MB. (2019). The Protection Potential of Antioxidant Vitamins Against Acute Respiratory Distress Syndrome: a Rat Trial. Inflammation. 42, 1585-1594.
- Goyal SN, Mahajan UB, Chandrayan G, Kumawat VS, Kamble S, Patil P, Agrawal YO, Patil CR, Ojha S. (2016). Protective effect of oleanolic acid on oxidative injury and cellular abnormalities in doxorubicin induced cardiac toxicity in rats. Am J Transl Res. 8, 60-69.
- Huang J, Wang Y, Li C, Wang X, He X. (2016). Anti-inflammatory oleanolic triterpenes from Chinese Acorns. Molecules. 21, 669.
- Hwang Y-J, Song J, Kim HR, Hwang KA. (2014). Oleanolic acid regulates NF-κB signaling by suppressing MafK expression in RAW 264.7 cells. BMB reports. 47, 524-529.
- Kısa A, Akyüz M, Çoğun HY, Kordali Ş, Bozhüyük AU, Tezel B, Şiltelioğlu U, Anıl B, Çakır A. (2018). Effects of Olea europaea L. leaf metabolites on the tilapia (Oreochromis niloticus) and three stored pests, Sitophilus granarius, Tribolium confusum and Acanthoscelides obtectus. Rec Nat Prod. 12, 201-215.
- Lee H, Lee IS, Choue R. (2013). Obesity, inflammation and diet. Pediatr Gastroenterol Hepatol Nutr. 16, 143-152.
- Liu J. (1995). Pharmacology of oleanolic acid and ursolic acid. J Ethnopharmacol. 49, 57-68.
- Liu J. (2005). Oleanolic acid and ursolic acid: research perspectives. J Ethnopharmacol. 100, 92-94.
- Marquez-Martin A, De La Puerta R, Fernandez-Arche A, Ruiz-Gutierrez V, Yaqoob P. (2006). Modulation of cytokine secretion by pentacyclic triterpenes from olive pomace oil in human mononuclear cells. Cytokine. 36, 211-217.
- Ohkawa H, Ohishi N, Yagi K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95, 351-358.
- Ozer EK, Goktas MT, Kilinc I, Pehlivan S, Bariskaner H, Ugurluoglu C, Iskit AB. (2017). Coenzyme Q10 improves the survival, mesenteric perfusion, organs and vessel functions in septic rats. Biomed Pharmacother. 91, 912-919.
- Petronilho F, Florentino D, Danielski LG, Vieira LC, Martins MM, Vieira A, Bonfante S, Goldim MP, Vuolo F. (2016). Alpha-lipoic acid attenuates oxidative damage in organs after sepsis. Inflammation. 39, 357-365.
- Reisman SA, Aleksunes LM, Klaassen CD. (2009). Oleanolic acid activates Nrf2 and protects from acetaminophen hepatotoxicity via Nrf2-dependent and Nrf2-independent processes. Biochem Pharmacol. 77, 1273-1282.
- Rocha M, Herance R, Rovira S, Hernández-Mijares A, Victor VM. (2012). Mitochondrial dysfunction and antioxidant therapy in sepsis. Infect Disord Drug Targets. 12, 161-78.
- Sedlak J, Lindsay RH. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 25, 192-205.
- Senthil S, Sridevi M, Pugalendi K. (2007). Cardioprotective effect of oleanolic acid on isoproterenol-induced myocardial ischemia in rats. Toxicol Pathol. 35, 418-423.
- Simmons J, Pittet JF. (2015). The coagulopathy of acute sepsis. Curr Opin Anaesthesiol. 28, 227-236.
- Singh GB, Singh S, Bani S, Gupta BD, Banerjee SK. (1992). Anti-inflammatory activity of oleanolic acid in rats and mice. J Pharm Pharmacol. 44, 456-458.
- Sun Y, Oberley LW, Li Y. (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem. 34, 497-500.
- Topçu G, Öztürk M, Kuşman T, Demirkoz AAB, Kolak U, Ulubelen A. (2013). Terpenoids, essential oil composition, fatty acid profile, and biological activities of Anatolian Salvia fruticosa Mill. Turk J Chem. 37, 619-632.
- Winters BD, Eberlein M, Leung J, Needham DM, Pronovost PJ, Sevransky JE. (2010). Long-term mortality and quality of life in sepsis: a systematic review. Crit Care Med. 38, 1276-1283.
- Yun N, Lee CH, Lee SM. (2009). Protective effect of Aloe vera on polymicrobial sepsis in mice. Food Chem Toxicol. 47, 1341-1348.
- Zhang J, Bi J, Liu S, Pang Q, Zhang R, Wang S, Liu C. (2017). 5-HT Drives Mortality in Sepsis Induced by Cecal Ligation and Puncture in Mice. Mediators Inflamm. 2017, 1-12.
- Zhou L, Gao M, Xiao Z, Zhang J, Li X, Wang A. (2015). Protective effect of astaxanthin against multiple organ injury in a rat model of sepsis. J Surg Res. 195, 559-567.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 31 Aralık 2020 |
| Gönderilme Tarihi | 9 Ekim 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 31 Sayı: 2 |
