The Protective Effect Mechanisms of Polyenylphosphatidylcholine PPC Pretreatment Against Stress Induced Ulcer in Rats

Year 2018, , 1 - 9, 01.01.2018

Necla Gürbüz Sarıkaş Melih Akın Kubilay Gürünlüoğlu Erkan Taş Aysun Bay Karabulut İclal Gürses

https://doi.org/10.5505/abantmedj.2018.82150

Abstract

INTRODUCTION: This study aims to investigate the mechanisms that are involved in protective actions exerted by pretreatment with polyenylphosphatidylcholine PPC against experimentally induced stress ulcer in rats.METHODS: Forty Swiss albino rats were divided into three groups. Group 1 n=10 was control, group 2 n=15 was stress ulcer and group 3 n=15 was PPC treated rats with stress ulcer. PPC treatment was started ten days before stress at a dose of 100 mg/day by oral route. Rats were terminated, stomachs were excised. Macroscopic ulcer index UI , production of reactive oxygen species, lipid peroxidation, plasma PGE2 and LTC4 levels, fatty-acid compositions were studied. The specimens were examined histopathologically.RESULTS: UI was significantly lower in the treatment group compared with non-treatment group. PGE2 decreased p=0.00 during cold restraint stress. PPC pretreatment was observed to inhibit the decrease in PGE2 in the induced stress ulcer. In the PPC treatment group LTC4 levels were significantly lower than non-treatment group p=0.00 . Additionally, PPC pretreatment inhibited degranülation of the submucosal mast cells. The inhibitory effect of PPC was more potent on red- stained mast cells than on blue-stained cells.DISCUSSION AND CONCLUSION: The present study shows that protective effects of PPC could be ascribed to inhibition of mast cell degranulation, a reduction in gastric oxidative injury, increase in biosynthesis of protective PGE2, and a decrease in biosynthesis of LTC4.

Keywords

Stress ulcer, Polyenylphosphatidylcholine, Prostaglandin E2, Leukotreine C4, Mast cell degranulation

Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları

Abstract

GİRİŞ ve AMAÇ: PPC ile prolifilaksinin, stres ülser gelişimini engellemede etkin olduğu daha önce yapılan deneysel bir çalışmada gösterilmiştir. Yine rat stres ülser modelinde gerçekleştirilen bu deneysel çalışmada, bu etkinliğin altında yatan mekanizmaların araştırılması amaçlanmıştır.YÖNTEM ve GEREÇLER: Ratlar, Grup1 n=10 kontrol, Grup2 n=15 stres ülser ve Grup3 n=15 PFK tedavisi + stres ülser olmak üzere üç gruba ayrıldı. Stres ülser modeli için Grup 2 ve grup 3 ratlar, 72 saatlik açlık periyodundan sonra, immobilize halde, +4 C?de, 4 saat soğuğa maruz bırakıldı. Grup 3 ratlara, PFK 100 mg/gün dozunda, oral yoldan, deney öncesi 10 gün süreyle verildi. Deney periyodu sonunda total çıkartılan ve büyük kurvatur boyunca açılan midelerde okülometrik yöntemle ülser indeksi UI hesaplandı Gastrik doku örneklerinde malondialdehit MDA ve superoksit dismutaz SOD , glutatyon GSH , ksantin oksidaz XO , eritrositlerde katalaz CAT , plazma örneklerinde ise total nitrit+nitrat, prostaglandin E2 PGE2 ve lökotrien C4 LTC4 düzeyleri ölçüldü. Histopatolojik incelemede gastrik mukozal hasar H&E boyama ve mast hücre degranülasyonu dominici boyama değerlendirildi.BULGULAR: Ülser indeksi UI ' nin PFK ile profilaksi grubunda, grup 2'den anlamlı düzeyde düşük olduğu görüldü p

Keywords

Stres ülser, Fosfotidilkolin, Prostaglandin E2, Lökotrien C4, Mast hücre degarnülasyonu

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