The investigatıon of the effects of the combined use of goji berry and propolis on gram-positive and gram-negative bacteria population

Öz

Our aim was to investigate how the combined use of red goji berry, which has antibacterial effects and is frequently preferred as a nutritional supplement, and lithium chloride (LiCl) which is used in the treatment of bipolar disorder and cancer, affect the antibacterial effects of each other on gram-positive and gram-negative bacteria. In this study, Escherichia coli (E.coli) and Salmonella typhi (S. typhi) were used as gram-negative bacteria and Staphylococcus aureus (S. aureus) as gram-positive bacteriaThese bacteria were grown on selective-mediums. The minimum inhibitory concentration 90 (MIC90) values of each bacterial strain were determined by the macrodilution test of goji berry and lithium chloride, and the MIC90 values of the antibiotics to which the bacteria were sensitive were determined by the microdilution test. Groups were determined as control (C), goji berry (G), lithium chloride (LiCl), the combination (G+LiCl) and the antibiotic groups (Amoxicillin, Ampicillin, Vancomycin). Whether the test substances changed the number of bacterial colonies was evaluated using the agar-dilution test. Each test group (n:3) was repeated 3 times. Biostatistical analysis was evaluated using Annova test and Tamhane’s T2 post-hoc test. MIC90 values of goji berry were determined as ≥50 mg/ml, ≥50 mg/ml and ≥100 mg/ml in E.coli, S.typhi and S.aerus, respectively. MIC90 values of LiCl were determined as ≥100mM, ≥150mM and ≥500mM in E.coli, S.typhi and S.aerus, respectively. MIC90 value of vancomycin was ≥1.5 µg/ml in S.aerus, amoxicillin ≥10 µg/ml in S.typhi, ampicillin ≥8 µg/ml E.coli. When compared to the control group, it was seen that all groups decreased the number of bacteria colonies. The colony numbers were determined as 300±7, 79±2, 72±3 in E.Coli, 300±6, 67±1,78±5, 77±3 in S. typhi, and 300±4, 116±6, 102±10, 101±5 in S. aureus for C, G,LiCl, G+LiCl groups, respectively.It was determined that the most resistant bacteria to LiCl was S.aerus, and the most sensitive bacteria was E.coli. In conclusion, it was determined that the combined use of LiCl and goji berry showed an antagonistic effect in this study.

Anahtar Kelimeler

• Goji berry
• Lithium chloride
• Antibacterial effect
• Food supplement

Goji Berry'nin, Lityum Klorür Birlikte Kullanımının Gram-Pozitif ve Gram-Negatif Bakteri Popülasyonu Üzerine Etkilerinin Araştırılması

Öz

Amacımız antibakteriyel etkilere sahip, besin takviyesi olarak sıkça tercih edilen kırmızı goji berry ile bipolar bozukluk ve kanser tedavisinde kullanılan lityum klorürün (LiCl) birlikte kullanımının birbirlerinin antibakteriyel etkilerini gram-pozitif ve gram negatif bakterilerde nasıl etkilediğini araştırmaktı. Bu çalışmada Escherichia coli (E.coli) ve Salmonella typhi (S. typhi) gram-negatif bakteri ve Staphylococcus aureus (S. aureus) gram-pozitif bakteri olarak kullanıldı. Bu bakteriler seçici-besiyerlerinde üretildi. Goji berry ve lityum klorürün makrodilüsyon testi ile her bir bakteri soyundaki minimum inhibitör konsantrasyon 90 (MİK90) değeri, bakterilerin duyarlı oldukları antibiyotiklerdeki MİK90 değerleri ise mikrodilüsyon testi ile saptandı. Gruplar kontrol (K), goji berry (G), lityum klorür (L), kombinasyon (G+L) ve antibiyotik grupları (Amoksisilin, Amfisilin, Vankomisin) olarak belirlendi. Bakteri kolonilerindeki sayı değişimi agar-dilüsyon testi kullanılarak değerlendirildi. Her test grubu (n:3) 3 defa tekrar edildi. Biyoistatistik analiz Annova testi ve Tamhane’s T2 post-hoc testi kullanılarak yapıldı. Goji berrynin MİK90 değerleri sırasıyla E.coli’de, S.typhi’de ve S.aerus’da, ≥50 mg/ml, ≥50 mg/ml ve ≥100 mg/ml olarak belirlendi. LiCl’ün MİK90 değerleri sırasıyla E.coli’de, S.typhi’de ve S.aerus’da ≥100mM, ≥150 mM ve 500 mM olarak belirlendi. Vankomisinin S.aerus’da ≥1.5 µg/ml, amoksisilinin S.typhi’de ≥10 µg/ml ve amfisilinin E.coli’de ≥8 µg/ml MİK90 değerleri saptandı. Kontrol grubuna göre kıyaslandığında tüm grupların, bakteri koloni sayılarını azalttığı görüldü. Koloni sayıları sırasıyla K, G, LiCl, G+LiCl gruplarında E.coli’de 300±7, 79±2, 72±3; S. typhi’de 300±6, 67±1,78±5, 77±3 ve S. aeurus’da 300±4, 116±6, 102±10, 101±5 olarak belirlendi. LiCl’e en dirençli bakterinin S. aeurus, en duyarlı bakterinin ise E.Coli olduğu saptandı. Bu çalışmada kombin LiCl ve goji berrynin kullanımının antagonist etki gösterdiği saptandı.

Anahtar Kelimeler

• Goji berry
• Lityum klorür
• Antibakteriyel etki
• Gıda takviyesi

Teşekkür

Çalışmada kullanılan bakterileri hibe eden ABMYO Gıda Teknolojisi Program Başkanı Dr. Öğretim Üyesi Ayla Ünver ALÇAY'a teşekkür ederiz.

Kaynakça

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