Adli Analizde Analitik Yöntem Doğrulaması

Öz

Adli bilimlerde toksikolojik bulguların doğru yorumlanması için güvenilir analitik verilere ihtiyaç vardır. Analitik veri güvenilir değilse, bu, sanık için yanlış hukuki süreçlere veya hastanın yanlış tedavisine yol açabilir. Bu nedenle, adli bilimlerde kullanılacak analitik yöntemlerin dikkatli bir şekilde yöntem geliştirilmesi ve doğrulanması gerekmektedir. Yöntem doğrulaması, referans yöntemlerinin oluşturulmasında ve bir laboratuvarın güvenilir analitik veri üretebilme yeteneğinin değerlendirilmesinde en önemli ve anahtar unsurdur. Yeni geliştirilmiş veya rutin olarak kullanılan analitik yöntemler, standart rehberlere göre doğrulanmalıdır. Analitik yöntem doğrulaması, seçicilik, özgüllük, doğruluk, hassasiyet, doğrusallık, aralık, tespit sınırı (LOD), nicelleme sınırı (LOQ), stabilite, sağlamlık ve robustluk gibi birkaç performans faktörünü içermelidir.

Anahtar Kelimeler

• Analitik Yöntemler
• Adli Bilimler
• Suç Analizi
• Doğrulama
• Belirleme
• Performans Özellikleri

Analytical Method Validation in Forensic Assay

Öz

Reliable analytical data is an essential requisite for accurate interpretation of toxicological findings in the forensic science. If the analytical data is not reliable, it may lead to wrong legal processes for the defendant or the wrong treatment of the patient. Therefore, analytical methods to be used in forensic science require careful method development and validation. Method validation is the most important and key element in establishing reference methods and evaluating the ability of a laboratory to produce reliable analytical data. Newly developed or routine used analytical methods must validate according to the standard guidelines. Analytical method validation should include several performance factors such as: Selectivity, specificity, accuracy, precision, linearity, range, limit of detection (LOD), limit of quantification (LOQ), stability, ruggedness, and robustness etc.

Anahtar Kelimeler

• Analytical methods
• forensic science
• criminal analysis
• validation
• determination
• performance characteristics

Kaynakça

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