Does Rectal Application of Red-Ginseng have an Effect on Colorectal Anastomosis Healing? An Experimental Study

Öz

Aim: To evaluate the possible effects of red-ginseng on colon anastomosis healing in an experimental rat model. Methods: In Group 1 (sham), laparotomy and colonic mobilization were performed. Colonic transection and anastomosis were performed to Groups 2 and 3. In Group 2 (control), 2 ml of saline was administered rectally to all rats for 10 days. Group 3 (drug group) rats were given 2.5 gr/kg ml red ginseng extract rectally for 10 days. Results: The rats in the sham group had the highest bursting pressure and the rats in the red ginseng group had higher bursting pressure than in the control group (p<0.05). A significant difference was observed only for the total SH level between the control group and the red ginseng group (p= 0.008). The red ginseng group had the highest scores for all parameters in the histopathological evaluation and the differences were significant compared to both the sham group and the control group. Conclusion: Rectally administered red-ginseng significantly increased wound healing parameters and increased anastomotic burst pressure. This is the first study in the literature on the beneficial effects of red-ginseng on colorectal anastomoses.

Anahtar Kelimeler

• Red-ginseng
• anastomosis
• antioxidant
• rat

Rektal Yoldan Uygulanan Red-Ginsengin Kolorektal Anastomoz İyileşmesinde Etkisi Var mıdır? Deneysel Çalışma

Öz

Amaç: Red-ginsengin kolon anastomozu iyileşmesindeki olası etkilerini değerlendirmek. Metot: Grup 1’de (sham) laparotomi ve kolonik mobilizasyon yapıldı. Diğer 2 grupta (grup 2+3) kolonik transeksiyon ve anastomoz yapıldı. Grup 2 (kontrol)’de tüm ratlara 10 gün boyunca rektal yoldan 2 ml saline verildi. Grup 3 (ilaç grubu)’de tüm ratlara 10 gün boyunca 2,5 gr/kg ml red ginseng ekstresi verildi. Sonuçlar: Sham grubundaki ratlar en yüksek patlama basıncına sahipti ve red ginseng grubundaki ratlar kontrol grubuna göre daha yüksek patlama basıncına sahipti (p<0.05). Kontrol grubu ile red ginseng grubu arasında sadece toplam SH düzeyi için anlamlı bir fark gözlendi (p= 0.008). Red ginseng grubu, histopatolojik değerlendirmede tüm parametreler için en yüksek puanlara sahipti ve farklılıklar hem sham grubu hem de kontrol grubu ile karşılaştırıldığında anlamlıydı. Sonuç: Rektal yoldan uygulanan red-ginseng belirgin olarak yara iyileşme parametrelerini arttırmış, anastomoz patlama basıncını arttırmıştır. Bu red-ginsengin kolorektal anastomozlar üzerindeki yararlı etkileri hakkında literatürdeki ilk çalışmadır.

Anahtar Kelimeler

• Red-ginseng
• anastomoz
• antioksidan
• rat

Kaynakça

1. 1) Kuru S, Kismet K, Bag YM, Barlas AM, Senes M, Durak M, et. al. Does the application of Ankaferd Blood Stopper rectally have positive effects on the healing of colorectal anastomosis and prevention of anastomotic leakage? An experimental study. Biomed Pharmacother. 2017;96:968-73.
2. 2) Barlas AM, Kuru S, Kismet K, Cavusoglu M, Bag YM, Senes M, et. al. Rectal application of argan oil improves healing of colorectal anastomosis in rats. Acta Cir Bras. 2018;33:565-76.
3. 3) Park GH, Park KY, Cho HI, Lee SM, Han JS, Won CH, et al. Red ginseng extract promotes the hair growth in cultured human hair follicles. J Med Food. 2015;18:354-62.
4. 4) Park KS, Park DH. The effect of Korean Red Ginseng on full-thickness skin wound healing in rats. J Ginseng Res. 2019 ;43:226-35.
5. 5) Wasowicz W, Nève J, Peretz A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem 1993;39:2522–6.
6. 6) Hadwan MH. Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC Biochemistry 2018; 19:7.
7. 7) Taylan E, Resmi H. The Analytical Performance of a Microplate Method for Total Sulfhydryl Measurement in Biological Samples. Turkish Journal of Biochemistry 2010; 35: 275-8.
8. 8) Ehrlich HP, Hunt TK. The effects of cortisone and anabolic steroids on the tensile strength of healing wounds. Ann Surg 1969; 170: 203-6.

9. 9) Philips JD, King CS, Fonkalsrud EW, Zeng H, Dindar H. Effects of chronic corticosteroid and vitamin A on the healing of intestinal anastomoses. Am J Surg 1992; 163: 71-7.
10. 10) Al-Khayal K, AlWesaimer AS, Abdulla M, Ahmed R. Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat Model. Dr. Sulaiman Al Habib Medical Journal 2020; 2:43-50.
11. 11) Ekici U, Ferhatoğlu MF, Çitgez B, Uludag M. Effects of the Folk Medicinal Plant Extract Ankaferd BloodStopper on the Healing of Colon Anastomosis: An Experimental Study in a Rat Model. Sisli Etfal Hastan Tip Bul. 2019;53:154-9.
12. 12) Aghayeva A, Benlice C, Bilgin IA, Atukeren P, Dogusoy G, Demir F, et al. The Effects of Hyperthermic Intraperitoneal Chemoperfusion on Colonic Anastomosis: An Experimental Study in a Rat Model. Tumori Journal. 2017;103:307-13.
13. 13) Arapoglou S, Kambaroudis A, Grivas I, Delis GA, Karkos C, Ballas K, et al. The Effect of Iloprost in the Healing of Colonic Anastomosis in Rats under Chemotherapy with Irinotecan. Chirurgia (Bucur). 2017;112:705-13.
14. 14) Ruiz-Luque V, Parra-Membrives P, Escudero-Severín C, Aguilar-Luque J. Effect of Alprostadil on Colorectal Anastomoses Under Relative Ischemia. J Surg Res. 2019 ;236:230-7.
15. 15) Ferhatoğlu MF, Kıvılcım T, Vural G, Kartal A, Filiz Aİ, Kebudi A, et al. Comparison of the effects of two different marine-derived omega-3 fatty acid sources, krill oil, and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model. Ulus Travma Acil Cerrahi Derg. 2019;25:324-30.
16. 16) Bae EA, Hyun YJ, Choo MK, Oh JK, Ryu JH, Kim DH. Protective effect of fermented red ginseng on a transient focal ischemic rats. Arch Pharm Res. 2004;27:1136-40.
17. 17) Durhan A, Koşmaz K, Şenlikci A, Erguder E, Suleyman M, Duymus ME, et al. Does red ginseng ameliorate liver damage caused by obstructive jaundice?: an experimental study. J Health Sci Med 2021; 4: 233-9.
18. 18) Koşmaz K , Durhan A , Şenlikci A , Suleyman M, Bostanci MT, Pekcici MR, et al. Evaluation of the Protective Effect of Red Ginseng on Lipid Profile, Endothelial and Oxidative Damage after Splenectomy in Rats. ACEM. 2021; 6: 43-9.