Hesperidin triggering apoptosis on neuroblastoma cell

Yıl 2018, Cilt: 3 Sayı:3, 123 - 126, 30.11.2018

Tolga Çatmakaş Barış Ertuğrul Elif Sinem İplik Bedia Çakmakoğlu

https://doi.org/10.25000/acem.449087

Öz

Aim: Neuroblastoma accounts for approximately %10
of all pediatric cancers and approximately %15 of cancer related deaths in
children. Understanding of the molecular mechanisms which play role in the
progress of this cancer type may lead to develop more effective strategies for
therapy. Flavanoids are popular subject for this new strategies. Hesperidin is an
herbal flavonoid which is found abundantly in citrus that has been studied on
several cancer cell lines. For this purpose, it was aimed to investigate is the
apoptotic effects of hesperidin on neuroblastoma tumors using SH-SY5Y cell line.

Methods: Hesperidin
was performed on SH-SY5Y and MRC-5 cell line by WST-1, Caspase-3 and
Annexin V in a time and dose dependent manner.

Results: 2.5 µM hesperidin and 5 µM hesperidin were
found the most suitable dosage for neuroblastoma cell line because of the
success on decreasing cell proliferation. Hesperidin has resulted with the
ability for apoptotic cell death compared with control group [MRC-5 cell line,
p<0.05 for all]. 2,5 µM and 5 µM hesperidin concentration for 48h were ended
up early apoptotic results as 53.65% for 2.5 µM and 38.90% for 5 µM. There was
no significant change on caspase-3 activity.

Conclusions: Our study suggests that hesperidin would be effective
against neuroblastoma tumors. We believe with further investigation this study
will be helpful for developing new research areas in neuroblastoma tumors.

Anahtar Kelimeler

Hesperidin, neuroblastoma, apoptosis

Nöroblastoma hücrelerinde hesperidinin apoptozu tetiklemesi

Öz

Amaç: Çocukluk
çağında rastlanan pediatrik kanserlerin yaklaşık %10’luk bir kısmını oluşturan nöroblastoma,
çocuklarda kansere bağlı ölümlerin yaklaşık %15’lik bir bölümünden de sorumludur.
Bu kanser türünün ilerlemesi esnasında rol alan moleküler olayların anlaşılması,
tedavi açısından daha etkili yöntemlerin ortaya konmasına aracılık
edebilecektir. Hesperidin turunçgillerde bolca bulunan bir flavonoiddir. Bu
amaçla, literatürde ilk olarak SH-SY5Y hücre hattı ve hesperidinin olası apoptotik
etkisini gösteren çalışma özelliği taşımaktadır.

Yöntem:
Hesperidinin SH-SY5Y hücre proliferasyonu ve canlılığı üzerinde doz bağımlı
etkisi için WST-1, kaspaz enzim aktivitesinin değerlendirilmesi için Kaspaz
3/BCA ve apoptotik değerlendirme için Annexin V analizleri yapılmıştır.

Bulgular: 2,5 µM hesperidin ve 5 µM hesperidin hücre
proliferasyonu üzerinde azalma etkisi yarattığından hücre hattında uygulanmak
üzere seçilmiştir. Hesperidin kontrol grubuna göre nöroblastoma üzerinde
apoptotik hücre ölümüne neden olmuştur [MRC-5 hücre hattı, hepsi için p<0.05].
2,5 µM hesperidin ve 5 µM hesperidin 48 saatlik inkübasyon sonucu %53,65 2,5 µM
için ve %38,90 5 µM için apoptoz ile sonuçlanmıştır. Kaspaz 3 aktivitesinde ise
herhangi bir değişim gözlenmemiştir.

Sonuç: Bu çalışmamızda elde ettiğimiz sonuçlardan,
nöroblastoma tümörlerinde hesperidinin antikarsinojenik etki gösterdiği
izlenimi edinilmiştir. Elde edilen verilerden yola çıkarak, çalışmamızın
yapılacak ileri araştırmalar ile birlikte nöroblastoma tümörlerinde yeni
araştırma alanlarının oluşmasında katkısı olabileceğini düşünmekteyiz.

Anahtar Kelimeler

nöroblastoma, hesperidin, apoptoz

Kaynakça

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