Background: Metabolic syndrome is associated with some medical disorders such as central obesity, being overweight, insulin resistance and hypertension. This study was designed to determine the effect of pioglitazone on oxidative stress in the insulin resistance rat model.
Methods: In this study, the model was induced by high sucrose (935 mm) diet for 20 weeks. Three groups were used in the experiment. Control group received standard laboratory diet and drinking water. Metabolic syndrome induced group received 32% sucrose containing drinking water for 20 weeks. Pioglitazone-treated metabolic syndrome group has received pioglitazone treatment (30 mg/kg/day, via oral gavage) for two weeks at the end of the 18th week of metabolic syndrome group. After experimental period, skeletal muscle tissues were homogenized to measure important enzymes such as aspartate aminotransferase, lactate dehydrogenase and as the marker of oxidative stress; total-antioxidant-status, total-oxidant-status and malondialdehyde. Western blot technique was used to determine protein level of thioredoxin1.
Results: Aspartate aminotransferase and lactate dehydrogenase levels increased in metabolic syndrome group but pioglitazone treatment decreased these levels. In metabolic syndrome group the oxidative stress status increased but the treatment of pioglitazone decreased the level of oxidative stress in the skeletal muscle. In addition, thioredoxin1 decreased in metabolic syndrome group but administration of pioglitazone increased this level.
Conclusions: There was an elevated effect of oxidative stress in high sucrose fed rats but the treatment of pioglitazone improved glucose tolerance and insulin sensitivity.
TUBITAK; Ankara Yıldırım Beyazıt University Projects Office
SBAG-115S827; 2864
I would like to thank Prof. Dr. Belma Turan for her departmental encouragement.
SBAG-115S827; 2864
Birincil Dil | İngilizce |
---|---|
Konular | Tıbbi ve Biyolojik Fizik |
Bölüm | ORIGINAL ARTICLE |
Yazarlar | |
Proje Numarası | SBAG-115S827; 2864 |
Yayımlanma Tarihi | 30 Eylül 2022 |
Gönderilme Tarihi | 8 Mart 2022 |
Yayımlandığı Sayı | Yıl 2022 |
Archives of Current Medical Research (ACMR) provides instant open access to all content, bearing in mind the fact that presenting research
free to the public supports a greater global exchange of knowledge.