Wet cupping therapy removes oxidative stress related miRNAs
Abstract
Background: Wet cupping therapy is commonly used in different conditions such as hypertension, diabetes, inflammatory and infectious diseases. The mechanism of action of wet cupping therapy is not yet clear, however several studies have demonstrated that it has a role in limiting oxidative stress. The aim of this study was to investigate the effect of wet cupping therapy on oxidative stress-related miRNAs miRNA-34a, miRNA-200a, miRNA-21 levels and oxidant-antioxidant status markers malondialdehyde (MDA) and glutathione (GSH) levels.
Methods: 60 healthy volunteer women aged 20-75 years (n=30, 20-45 years; n=30, 45-75 years) who were applied wet cupping at Konya Necmettin Erbakan University Meram Medical Faculty Traditional and Complementary Medicine Center were included in this study. Plasma miRNA-34a, miRNA-200a and miRNA-21 levels were analyzed by using real-time quantitative PCR (RT-PCR) analysis. MDA and GSH levels were measured using the commercial ELISA kits.
Results: MDA levels [2003.10 (1810.17-7392.15) vs 1884.04 (1800.10-4488.05), p=0.027] of wet cupping blood samples higher than venous blood samples, while GSH levels [125.97 (90.65-219.43) vs 131.77 (88.77-246.42), p=0.037] were lower. miRNA-34a [(0.94±0.74) vs (0.53±0.28), p<0.001], miRNA-21 [0.058 (0.01-0.54) vs 0.033 (0.01±0.18), p=0.001] and miRNA-200a [24.42 (7.46-103.97) vs 20.32 (4.32-56.49), p=0.037] levels of wet cupping blood samples were significantly higher than venous blood samples.
Conclusions: Wet cupping therapy eliminates oxidative stress-related miRNAs from the body. Therefore, it is seen as a promising method in the welfare of reactive oxygen species (ROS)-related diseases by limiting oxidative damage.
Keywords
Cupping theraphy , miRNAs , oxidative stress , reactive oxygen species.
Kaynakça
- Mehta P, Dhapte V. Cupping therapy: A prudent remedy for a plethora of medical ailments. J Tradit Complement Med. 2015;5(3):127-34.
- Aboushanab TS, AlSanad S. Cupping therapy: An overview from a modern medicine perspective. J Acupunct Meridian Stud. 2018;11(3):83-87.
- Li JQ, Guo W, Sun ZG, Huang QS, Lee EY, Wang Y, et al. Cupping therapy for treating knee osteoarthritis: The evidence from systematic review and meta-analysis. Complement Ther Clin Pract. 2017;28:152- 60.
- Al-Bedah AMN, Elsubai IS, Qureshi NA, Aboushanab TS, Ali GIM, El- Olemy AT, et al. The medical perspective of cupping therapy: Effects and mechanisms of action. J Tradit Complement Med. 2019;9(2):90-97.
- Umar NK, Tursunbadalov S, Surgun S, Welcome MO, Dane S. The effects of wet cupping therapy on the blood levels of some heavy metals: A pilot study. J Acupunct Meridian Stud. 2018;11(6):375-79.
- Al-Bedah A, Aboushanab T, Alqaed M, Qureshi N, Sohaibani I, Ali G, et al. Classification of cupping therapy: A tool for modernization and standardization. J Complement Med Res. 2016;1:1-10.
- Ma SY, Wang Y, Xu JQ, Zheng L. Cupping therapy for treating ankylosing spondylitis: The evidence from systematic review and meta-analysis. Complement Ther Clin Pract. 2018;32:187-94.
- El Sayed SM, Mahmoud HS, Nabo MMH. Methods of wet cupping therapy (Al-Hijamah): In light of modern medicine and prophetic medicine. Altern Integ Med. 2013;2(3):1-16.
- Al-Bedah AMN, Elsubai IS, Qureshi NA, Aboushanab TS, Ali GIM, El- Olemy AT, et al. The medical perspective of cupping therapy: Effects and mechanisms of action. J Tradit Complement Med. 2018;9(2):90-97
- Al-Tabakha MM, Sameer FT, Saeed MH, Batran RM, Abouhegazy NT, Farajallah AA. Evaluation of bloodletting cupping therapy in the management of hypertension. J Pharm Bioallied Sci. 2018;10(1):1-6.