Background: Metabolic syndrome is associated with some medical disorders such as central obesity, being overweight, insulin resistance and hypertension. This study was designed to determine the effect of pioglitazone on oxidative stress in the insulin resistance rat model.
Methods: In this study, the model was induced by high sucrose (935 mm) diet for 20 weeks. Three groups were used in the experiment. Control group received standard laboratory diet and drinking water. Metabolic syndrome induced group received 32% sucrose containing drinking water for 20 weeks. Pioglitazone-treated metabolic syndrome group has received pioglitazone treatment (30 mg/kg/day, via oral gavage) for two weeks at the end of the 18th week of metabolic syndrome group. After experimental period, skeletal muscle tissues were homogenized to measure important enzymes such as aspartate aminotransferase, lactate dehydrogenase and as the marker of oxidative stress; total-antioxidant-status, total-oxidant-status and malondialdehyde. Western blot technique was used to determine protein level of thioredoxin1.
Results: Aspartate aminotransferase and lactate dehydrogenase levels increased in metabolic syndrome group but pioglitazone treatment decreased these levels. In metabolic syndrome group the oxidative stress status increased but the treatment of pioglitazone decreased the level of oxidative stress in the skeletal muscle. In addition, thioredoxin1 decreased in metabolic syndrome group but administration of pioglitazone increased this level.
Conclusions: There was an elevated effect of oxidative stress in high sucrose fed rats but the treatment of pioglitazone improved glucose tolerance and insulin sensitivity.
|Destekleyen Kurum||TUBITAK; Ankara Yıldırım Beyazıt University Projects Office|
|Proje Numarası||SBAG-115S827; 2864|
|Teşekkür||I would like to thank Prof. Dr. Belma Turan for her departmental encouragement.|
|Yayımlanma Tarihi||30 Eylül 2022|
|Başvuru Tarihi||8 Mart 2022|
|Kabul Tarihi||19 Temmuz 2022|
|Yayınlandığı Sayı||Yıl 2022, Cilt 3, Sayı 3|
|APA||Bilginoğlu, A. & Tutar Selçuk, M. F. (2022). Effect of pioglitazone on oxidative stress of skeletal muscle in the insulin resistance rat model induced by high sucrose diet . Archives of Current Medical Research , 3 (3) , 241-246 . Retrieved from https://dergipark.org.tr/tr/pub/acmr/issue/72847/1084437|
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