The Investigation of the Presence and Antimicrobial Profiles of Arcobacter Species in Sheep Carcasses and Feces

Yıl 2019, Cilt: 45 Sayı: 2, 42 - 49, 01.05.2019

Cansu Çelik , Serkan İkiz

Öz

DOI: 10.26650/actavet.2019.18007

This study was designed to investigate the
presence and the prevalence of Arcobacter butzleri, Arcobacter cryaerophilus,
and Arcobacter skirrowii in sheep carcass swabs and feces from sheep with and
without diarrhea raised in Istanbul. Also it was aimed to determine their
resistance profiles against some antimicrobials.  For this purpose, 50 fecal samples from sheep
without diarrhea, 50 fecal samples from sheep with diarrhea, and 50 carcass
swab samples from sheep were the material of this study. Arcobacter spp. were
isolated from 49  (32.6%)  of total 150 samples. 34 samples (68%) of 50
fecal samples from sheep with diarrhea, 5 samples (10%) from 50 fecal samples
of sheep without diarrhea and 10 samples (20%) of 50 sheep carcass swabs were
found to be positive according to isolation results. According to multiplex Polymerase
Chain Reaction (mPCR) results, 31 of 49 were identified as A. skirrowii
(63.3%), 9 of  49 were A. butzleri
(18.3%), while 9 of 49 were A. cryaerophilus (18.3%). As a conclusion,
Arcobacter species should be taken into consideration especially in sheep with
diarrhea. Additionally, it should be considered that arcobacters have started
to gain resistance against fluoroquinolones. 

Cite this article as: Çelik, C., İkiz, S., 2019. The Investigation of the Presence and Antimicrobial Profiles of Arcobacter Species in Sheep Carcasses and Feces. Acta Vet Eurasia 2019; DOI: 10.26650/actavet.2019.18007

Anahtar Kelimeler

Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, sheep, mPCR, antibiogram

Kaynakça

• 1.Abay S., Kayman T., Hizlisoy H., Aydin F., 2012. In vitro antibacterial susceptibility of Arcobacter butzleri isolated from different sources. Journal of Veterinary Medical Science 74(5), 613-616.
• 2.Aski H.S., Tabatabaei M., Khoshbakht R., Raeisi M., 2016. Occurrence and antimicrobial resistance of emergent Arcobacter spp. isolated from cattle and sheep in Iran. Comparative Immunology, Microbiology and Infectious Diseases 44, 37-40.
• 3.Assanta M,A., Roy D., Lemay M.J., Montpetit D., 2002. Attachment of Arcobacter butzleri, a new waterborne pathogen, to water distribution pipe surfaces. Journal of Food Protection 65(8), 1240-1247.
• 4.Atabay H. I., & Corry, J. E., 1998. Evaluation of a new arcobacter enrichment medium and comparison with two media developed for enrichment of Campylobacter spp. International Journal of Food Microbiology 41(1), 53-58.
• 5.Cardoen S., Van Huffel X., Berkvens D., et al., 2009. Evidence-based semiquantitative methodology for prioritization of foodborne zoonoses. Foodborne Pathogens and Disease 6, 1083-1095.
• 6.Collado L., Figueras M.J., 2011. Taxonomy, epidemiology and clinical relevance of the genus Arcobacter. Clinical Microbiology Reviews 24 (1), 174–192.
• 7.Ertaș N., Doğruer Y., 2009. Prevalence of Arcobacter species in ground meat from cattle and sheep using multiplex PCR techniques. Sağlık Bilimleri Veteriner Dergisi, Fırat Üniversitesi 23(2), 95-100.
• 8.Fernandez H., Villanueva M.P., Mansilla I., Gonzalez M., Latif F., 2015. Arcobacter butzleri and A. cryaerophilus in human, animals and food sources, in southern Chile. Brazilian Journal of Microbiology 46(1), 145-147.
• 9.Golla S.C., Murano E.A., Johnson L.G., Tipton N.C., Cureington E.A., Savell J.W., 2002. Determination of the occurrence of Arcobacter butzleri in beef and dairy cattle from Texas by various isolation methods. Journal of Food Protection 65(12), 1849-1853.
• 10.Grove-White D.H., Leatherbarrow A.J.H., Cripps P.J., Diggle P.J., French N.P., 2014. Temporal and farm-management-associated variation in faecal pat prevalence of Arcobacter spp. in ruminants. Epidemiology & Infection 142(4), 861-870.
• 11.Houf K., Tutenel A., de Zutter L., Hoof J.V., Vandamme P., 2000. Development of a multiplex PCR assay for simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. FEMS Microbiology Letters 193, 89-94.
• 12. Houf K., Devriese L.A., De Zutter L., Van Hoof J., Vandamme P., 2001. Development of a new protocol for the isolation and quantification of Arcobacter species from poultry products. International Journal of Food Microbiology 71(2-3), 189-196.
• 13.Houf K., De Zutter L., Van Hoof J., Vandamme P., 2002. Assessment of the genetic diversity among Arcobacters isolated from poultry products by using two PCR- based typing methods. Applied Enviromental Microbiology 68, 2172-2178.
• 14.Houf K., Devriese L.A., Haesebrouck F., Vandenberg O., Butzler J.P., Hoof J.V., Vandamme P., 2004. Antimicrobial susceptibility patterns of Arcobacter butzleri and Arcobacter cryaerophilus strains isolated from humans and broilers. Microbial drug resistance 10(3), 243-247.
• 15.Kabeya H., Maruyama S., Morita Y., Kubo M., Yamamoto K., Arai S., Izumi T., Kobayashi Y., Katsube Y., Mikami T., 2003. Distribution of Arcobacter species among livestock in Japan. Veterinary Microbiology 93(2), 153-158.
• 16.Kemp R., Leatherbarrow A.J., Williams N.J., Hart C.A., Clough H.E., Turner J., Wright E.J., French N.P., 2005. Prevalence and genetic diversity of Campylobacter spp. in environmental water samples from a 100-square-kilometer predominantly dairy farming area. Applied and Environmental Microbiology 71(4), 1876-1882.
• 17.Lau S.K., Woo PC, Teng J.L., Leung K.W., Yuen K.Y., 2002. Identification by 16S ribosomal RNA gene sequencing of Arcobacter butzleri bacteraemia in a patient with acute gangrenous appendicitis. Molecular Pathology 55(3), 182.
• 18.Luber P., Bartelt E., Genschow E., Wagner J., Hahn H., 2003. Comparison of broth microdilution, E Test, and agar dilution methods for antibiotic susceptibility testing of Campylobacter jejuni and Campylobacter coli. Journal of Clinical Microbiology 41(3), 1062-1068.
• 19.Merga J.Y., Leatherbarrow A.J., Winstanley C., Bennett M., Hart C.A., Miller W.G., Williams N.J., 2011. Comparison of Arcobacter isolation methods, and diversity of Arcobacter spp. in Cheshire, United Kingdom. Applied and Environmental Microbiology 77(5), 1646-1650.
• 20.Milesi, S., 2011. Emerging pathogen Arcobacter spp. in food of animal origin. Doctoral thesis, University of Milan, Milan.
• 21.Nachamkin, I., Szymanski, C. M., & Blaser, M. J., 2008. Campylobacter (No. Ed. 3). ASM Press.
• 22.Neill S.D., Ellis W.A.. O’Brien J.J., 1978. The biochemical characteristics of Campylobacter-like organisms from cattle and pigs. Research in Veterinary Science 25(3), 368-372.
• 23.Neubauer C., Hess M., 2006. Detection and identification of food-borne pathogens of the genera Campylobacter, Arcobacter and Helicobacter by multiplex PCR in poultry and poultry products. Journal of Veterinary Medicine Series B, 53(8), 376-381.
• 24.On S.L., Jensen T.K., Bille-Hansen V., Jorsal S.E., Vandamme P., 2002. Prevalence and diversity of Arcobacter spp. isolated from the internal organs of spontaneous porcine abortions in Denmark. Veterinary Microbiology 85(2), 159-167.
• 25.Pérez-Cataluña A., Tapiol J., Benavent C., Sarvisé C., Gómez F., Martínez B., Terron-Puig M., Recio G., Vilanova A., Pujol I., Ballester F., 2017. Antimicrobial susceptibility, virulence potential and sequence types associated with Arcobacter strains recovered from human faeces. Journal of Medical Microbiology 66(12), 1736-1743.
• 26.Rahimi E., 2014. Prevalence and antimicrobial resistance of Arcobacter species isolated from poultry meat in Iran. British Poultry Science 55(2), 174-180.
• 27.Ramees T.P., Dhama K., Karthik K., Rathore R.S., Kumar A., Saminathan M., Tiwari R., Malik Y.S., Singh R.K., 2017. Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control–a comprehensive review. Veterinary Quarterly 37(1), 136-161.
• 28. Sekhar, M.S., Tumati, S.R., Chinnam, B.K., Kothapalli, V.S., & Sharif, N.M., 2017. Virulence gene profiles of Arcobacter species isolated from animals, foods of animal origin, and humans in Andhra Pradesh, India. Veterinary world, 10(6), 716.
• 29.Snelling W.J., Matsuda M., Moore J.E., Dooley J.S., 2006. Under the microscope: Arcobacter. Letters in Applied Microbiology 42(1), 7-14.
• 30.Son I., Englen M.D., Berrang M.E., Fedorka-Cray P.J., Harrison M.A., 2007. Antimicrobial resistance of Arcobacter and Campylobacter from broiler carcasses. International Journal of Antimicrobial Agents 29(4), 451-455.
• 31.Sürmeli P., 2006. Kars ili ve çevresinde yetiştirilen koyunlardan alınan dışkı örneklerinde Arcobacter türlerinin prevalansının araştırılması. Master thesis, Kafkas University, Faculty of Veterinary Medicine, Department of Microbiology, Institute of Health Sciences, Kars.
• 32.Ünver A., Atabay H.I., Şahin M., Çelebi Ö., 2013. Antimicrobial susceptibilities of various Arcobacter species. Turkish Journal of Medical Sciences 43(4), 548-552.
• 33.Van Driessche E., Houf K., Hoof J.V., De Zutter L., Vandamme P., 2003. Isolation of Arcobacter species from animal feces. FEMS Microbiology Letters 229, 243-248.
• 34.Van Driessche E., Houf K., Vangroenweghe F., De Zutter L., Van Hoof J., 2005. Prevalence, enumeration and strain variation of Arcobacter species in the faeces of healthy cattle in Belgium. Veterinary microbiology 105(2), 149-154.
• 35.Vandamme P., De Ley J., 1991. Proposal for a new family, Campylobacteraceae. International Journal of Systematic and Evolutionary Microbiology 41(3), 451-455.
• 36.Vandamme P., Vancanneyt M., Pot B., Mels L., Hoste B., Dewettinck D., Vlaes L., Van Den Borre C., Higgins R., Hommez J., Kersters K., 1992a. Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. International Journal of Systematic and Evolutionary Microbiology 42(3), 344-356
• 37.Vandamme P., Pugina P., Benzi G., Van Etterijck R., Vlaes L., Kersters K., ... & Lauwers S., 1992b. Outbreak of recurrent abdominal cramps associated with Arcobacter butzleri in an Italian school. Journal of Clinical Microbiology 30(9), 2335-2337.
• 38.Vandenberg O., Houf K., Douat N., Vlaes, L., Retore P., Butzler J.P., Dediste A., 2006. Antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from Belgium. Journal of Antimicrobial Chemotherapy 57(5), 908-913.