Rhododendron luteum ve Rhododendron ponticum Çiçek Özlerinin Artemia salina Larvaları Üzerindeki Potansiyel Toksisitesinin ve Biyokimyasal Aktivitelerinin Karşılaştırmalı Değerlendirilmesi

Yıl 2024, Cilt: 10 Sayı: 1, 39 - 50

Yeşim Dağlıoğlu

Öz

Bu çalışmanın odak noktası, Artemia salina (tuzlu su karidesi) instar IV larvaları üzerindeki farklı Rhododendron türlerinin (Rhododendron luteum ve Rhododendron ponticum) farklı konsantrasyonlarda toksisitelerini karşılaştırmalı araştırmaktır. Çünkü, Rhododendron türleri biyoaktif bileşiklerce zengin bir familyaya aittir ki bu da onları oldukça zehirli hale getirmektedir. İki farklı Rhododendron türlerinin instar IV larvaların ölümü oranı üzerinde etkisini, oksidatif stres oluşturma potansiyelini ve larvaların savunma kapasitesini araştırdık. Larvalar hücre kültür plaklarında 48-96 saat boyunca yapay deniz suyunda Rhododendron özütlerine farklı konsantrasyonlarda maruz bırakıldı. Stereomikroskop altında her bir konsantrasyonda ölüm oranı ve morfolojik değişklikler değerlendirildi. Çalışmamızda, Rhododendron türlerinin A. salina instar IV larvaları üzerinde oldukça toksik olduğu ve yine R. ponticum türünün R. luteum'a göre daha toksik etkili olduğu görülmüştür. Özellikle, 200 µg/mL ve üstü konsantrasyonda her iki Rhododendron türünde hücre hasarını göstren MDA seviyesi anlamılı bir şekilde artmıştır (P<0.05). Buna karşın oksidatif stresin etkisini azaltmak için savunma stratejisi olan SOD seviyesi Rhododendron türlerinde artan konsantrasyonla azalmakla birlikte sadece 800-3200 µg/mL konsantrasyon aralığında anlamlıdır (P<0,05).

Anahtar Kelimeler

MDA, oksidatif stres, Rhododendron sp., SOD, toksisite.

Destekleyen Kurum

ORDU ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNASYON BİRİMİ

Teşekkür

This study was supported by Ordu University Scientific Research Projects Coordination Unit (ODÜ, BAP, Project Number: B-2318).

Comparative Assessment of Potential Toxicity and Biochemical Properties of Rhododendron luteum and Rhododendron ponticum Flower Extracts on Artemia salina

Öz

The focus of this study was to comparatively investigate the toxicity of different Rhododendron species (Rhododendron luteum and Rhododendron ponticum) at different concentrations on instar IV larvae of Artemia salina (Brine shrimp). Because Rhododendron species belong to a Ericaceae family rich in bioactive compounds, which makes them highly poisonous. We investigated the effect of two different Rhododendron species on the mortality rate of instar IV larvae, the potential to induce oxidative stress, and the defense capacity of the larvae. Larvae were exposed to different concentrations of Rhododendron extracts in artificial seawater for 48–96 h in cell culture plates. Mortality rate and morphological changes were evaluated at each concentration under a stereomicroscope. In our study, Rhododendron species were found to be highly toxic to A. salina instar IV larvae and R. ponticum was more toxic than R. luteum. Particularly, the level of MDA, which indicates cell damage, increased significantly in both Rhododendron species at a concentration of 200 µg/mL and above (P<0.05). On the other hand, the level of SOD, which is a defense strategy to reduce the effect of oxidative stress, decreased with increasing concentration in Rhododendron species, but was significant only in the concentration range of 800-3200 µg/mL (P<0.05).

Anahtar Kelimeler

MDA, oxidative stress, Rhododendron sp. SOD, toxicity

Kaynakça

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