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Effects of Mucoadhesive Gel Containing Propolis on Some Biochemical and Hematologic Parameters in Rats With Experimental Periodontitis

Yıl 2022, , 44 - 49, 28.12.2022
https://doi.org/10.53913/aduveterinary.1185773

Öz

This study aimed to investigate the effects of propolis-containing mucoadhesive gel in experimentally induced periodontitis in rats. Propolis-containing mucoadhesive gel was prepared by using chitosan with a modified mechanical method. Thirty-five Sprague Dawley rats were used in the study. Rats were divided five groups as negative control, periodontitis + 50 mg/dl propolis, periodontitis + 100 mg/dl propolis, periodontitis + chitosan and healthy control. Experimental periodontitis was induced by placing ligatures on the inferior frontal teeth. After 11 days, the ligatures were removed, and gel applications were started. On the eighth day, blood samples were taken under anaesthesia. Haematological and biochemical analyses were performed from whole blood and serum samples. As a result of the statistical analysis, non-statistically significant decreases were determined in serum CRP, IL-1, IL-6 and TNF-α levels in the groups that were applied a mucoadhesive gel containing propolis. As a result, it was thought that mucoadhesive gel containing propolis might help treat periodontitis.

Destekleyen Kurum

ADÜ-BAP

Proje Numarası

VTF-21025

Kaynakça

  • Aral, C. A., Kesim, S., Greenwell, H., Kara, M., Çetin, A., & Yakan, B. (2015). Alveolar bone protective and hypoglycemic effects of systemic propolis treatment in experimental periodontitis and diabetes mellitus. Journal of Medicinal Food, 18(2), 195-201. https://doi: 10.1089/jmf.2013.3137
  • Bansal, T., Pandey, A., Deepa, D., & Asthana, A. K. (2014). C-reactive protein (CRP) and its association with periodontal disease: a brief review. Journal of clinical and diagnostic research: JCDR, 8(7), ZE21. https://doi: 10.7860/JCDR/2014/8355.4646
  • Belkaid, Y., & Hand, T. W. (2014). Role of the microbiota in immünity and inflammation. Cell, 157(1), 121-141. https://doi.org/10.1016/j.cell.2014.03.011
  • Boddupalli, B. M., Mohammed, Z. N., Nath, R. A., & Banji, D. (2010). Mucoadhesive drug delivery system: An overview. Journal of advanced pharmaceutical technology & research, 1(4), 381.https://doi: 10.4103/0110-5558.76436
  • Borrelli, F., Maffia, P., Pinto, L., Ianaro, A., Russo, A., Capasso, F. & Ialenti, A.(2002). Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia, 73(1), 53-63. https://doi.org/10.1016/S0367-326X(02)00191-0
  • Botelho, J., Leira, Y., Viana, J., Machado, V., Lyra, P., Aldrey, J. M., ... & Mendes, J. J. (2021). The Role of Inflammatory Diet and Vitamin D on the Link between Periodontitis and Cognitive Function: A Mediation Analysis in Older Adults. Nutrients, 13(3), 924. https://doi.org/ 10.3390/nu13030924
  • Castaldo, S., & Capasso, F. (2002). Propolis, an old remedy used in modern medicine. Fitoterapia, 73, S1-S6. https://doi.org/10.1016/S0367-326X(02)00185-5Dietrich, T., Sharma, P., Walter, C., Weston, P., & Beck, J. (2013). The epidemiological evidence behind the association between periodontitis and incident atherosclerotic cardiovascular disease. Journal of periodontology, 84, S70-S84. https://doi:10.1902/jop.2013.134008
  • Eroğlu, H. E., Tatlışen, A., & Özkul, Y. (2004). Mesane kanserli doku kültürlerindeki mikronükleus üzerine propolis ve mitomisin-c’nin etkileri. Erciyes Üniversitesi Sağlık Bilimleri Dergisi (E.Ü.Journal of Health Sciences) 13(2) 15-20
  • Farquharson, D., Butcher, J. P., & Culshaw, S. (2012). Periodontitis, Porphyromonas, and the pathogenesis of rheumatoid arthritis. Mucosal immünology, 5(2), 112–120. https://doi:10.1038/mi.2011.66
  • Graves, D. T., Kang, J., Andriankaja, O., Wada, K., & Rossa Jr, C. (2012). Animal models to study host-bacteria interactions involved in periodontitis. Periodontal Disease, 15, 117-132. https://doi: 10.1159/000329675
  • Hajishengallis, G., & Chavakis, T. (2021). Local and systemic mechanisms linking periodontal disease and inflammatory comorbidities. Nature Reviews Immunology, 1-15. https://doi.org/10.1038/ s41577-020-00488-6
  • Ikeno K, Ikeno T, Miyazawa C. Effects of propolis on dental caries in rats. Caries Research. 1991; 25(5):347-51. https://doi.org/10.1159/000261390
  • Koo, H., Gomes, B. P. F. A., Rosalen, P. L., Ambrosano, G. M. B., Park, Y. K., & Cury, J. A. (2000). In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Archives of oral biology, 45(2), 141-148. https://doi.org/10.1016/S0003-9969(99)00117-X
  • Koru, O., Toksoy, F., Acikel, C.H., Tunca, Y.M., Baysallar, M., Guclu, A.U. (2007). In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe. 13(3):140–5. https://doi.org/10.1016/j.anaerobe.2007.02.001
  • Kujumgiev, A., Tsvetkova, I., Serkedjieva, Y., Bankova, V., Christov, R., Popov, S.(1999). Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of ethnopharmacology. 64(3):235-40. https://doi.org/10.1016/S0378-8741(98)00131-7
  • Nishihara, R., Sugano, N., Takano, M., Shimada, T., Tanaka, H., Oka, S., & Ito, K. (2009). The effect of Porphyromonas gingivalis infection on cytokine levels in type 2 diabetic mice. Journal of periodontal research, 44(3), 305-310. doi:10.1111/j.1600-0765.2008.01130.x
  • Olsen, I. (2015). From the acta prize lecture 2014: The periodontal-systemic connection seen from a microbiological standpoint: Summary of the Acta Odontologica Scandinavia Price lecture 2014 presented at the meeting of the IADR/Pan European region in Dubrovnik, September 10–13. 2014. Acta Odontologica Scandinavica, 73(8), 563-568. https://doi.org/10.3109/00016357.2015.1007480
  • Orsi, R., Sforcin, J., Rall, V., Funari, S., Barbosa, L., Fernandes, J. (2005). Susceptibility profile of Salmonella against the antibacterial activity of propolis produced in two regions of Brazil. Journal of Venomous Animals and Toxins including Tropical Diseases, 11(2), 109-116.
  • Paraskevas, S., Huizinga, J. D., & Loos, B. G. (2008). A systematic review and meta‐analyses on C‐reactive protein in relation to periodontitis. Journal of clinical periodontology, 35(4), 277-290. https://doi: 10.1111/j.1600-051X.2007.01173.x
  • Popova, M., Silici, S., Kaftanoglu, O., & Bankova, V. (2005). Antibacterial activity of Turkish propolis and its qualitative and quantitative chemical composition. Phytomedicine, 12(3), 221-228. https://doi.org/10.1016/j.phymed.2003.09.007
  • Poppe, B. & Michaelis, H. (1986). Results of a twice- yearly controlled oral hygiene activity using a propolis-containing toothpaste (double-blind study). Stomatologie der DDR, 36(4), 195-203.
  • Preshaw, P.M. & Taylor, J.J.(2011). How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol, 38(11), 60-84. https://doi: 10.1111/j.1600-051X.2010.01671.x
  • Ryder, M. I. (2010). Comparison of neutrophil functions in aggressive and chronic periodontitis. Periodontology 2000, 53(1), 124–137.
  • Seidel V, Peyfoon E, Watson DG, Fearnley J. Comparative study of the antibacterial activity of propolis from different geographical and climatic zones. Phytotherapy Research. 2008;22(9):1256-63. DOI: 10.1002/ptr.2480
  • Singh, M., Briones, M., & O’Hagan, D. T. (2001). A novel bioadhesive intranasal delivery system for inactivated influenza vaccines. Journal of controlled release, 70(3), 267-276. https://doi.org/10.1016/S0168-3659(00)00330-8
  • Skaba, D., Morawiec, T., Tanasiewicz, M., Mertas, A., Bobela, E., Szliszka, E., Król, W. (2013). Influence of the toothpaste with Brazilian ethanol extract propolis on oral cavity health. Evidence-Based Complementary and Alternative Medicine. Article ID 215391 | https://doi.org/10.1155/2013/215391
  • Struillou, X., Boutigny, H., Soueidan, A., & Layrolle, P. (2010). Experimental animal models in periodontology: a review. The open dentistry journal, 4, 37. 10.2174/1874210601004010037
  • Takano, M., Nishihara, R., Sugano, N., Matsumoto, K., Yamada, Y., Takane, M., ... & Ito, K. (2010). The effect of systemic anti-tumour necrosis factor-alpha treatment on Porphyromonas gingivalis infection in type 2 diabetic mice. Archives of oral biology, 55(5), 379-384. https://doi.org/10.1016/j.archoralbio.2010.03.004
  • Velazquez C, Navarro M, Acosta A, Angulo A, Dominguez Z, Robles R, et al. Antibacterial and free-radical scavenging activities of Sonoran propolis. Journal of Applied Microbiology. 2007; 103(5):1747-56. doi:10.1111/j.1365-2672.2007.03409.x
Yıl 2022, , 44 - 49, 28.12.2022
https://doi.org/10.53913/aduveterinary.1185773

Öz

Proje Numarası

VTF-21025

Kaynakça

  • Aral, C. A., Kesim, S., Greenwell, H., Kara, M., Çetin, A., & Yakan, B. (2015). Alveolar bone protective and hypoglycemic effects of systemic propolis treatment in experimental periodontitis and diabetes mellitus. Journal of Medicinal Food, 18(2), 195-201. https://doi: 10.1089/jmf.2013.3137
  • Bansal, T., Pandey, A., Deepa, D., & Asthana, A. K. (2014). C-reactive protein (CRP) and its association with periodontal disease: a brief review. Journal of clinical and diagnostic research: JCDR, 8(7), ZE21. https://doi: 10.7860/JCDR/2014/8355.4646
  • Belkaid, Y., & Hand, T. W. (2014). Role of the microbiota in immünity and inflammation. Cell, 157(1), 121-141. https://doi.org/10.1016/j.cell.2014.03.011
  • Boddupalli, B. M., Mohammed, Z. N., Nath, R. A., & Banji, D. (2010). Mucoadhesive drug delivery system: An overview. Journal of advanced pharmaceutical technology & research, 1(4), 381.https://doi: 10.4103/0110-5558.76436
  • Borrelli, F., Maffia, P., Pinto, L., Ianaro, A., Russo, A., Capasso, F. & Ialenti, A.(2002). Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia, 73(1), 53-63. https://doi.org/10.1016/S0367-326X(02)00191-0
  • Botelho, J., Leira, Y., Viana, J., Machado, V., Lyra, P., Aldrey, J. M., ... & Mendes, J. J. (2021). The Role of Inflammatory Diet and Vitamin D on the Link between Periodontitis and Cognitive Function: A Mediation Analysis in Older Adults. Nutrients, 13(3), 924. https://doi.org/ 10.3390/nu13030924
  • Castaldo, S., & Capasso, F. (2002). Propolis, an old remedy used in modern medicine. Fitoterapia, 73, S1-S6. https://doi.org/10.1016/S0367-326X(02)00185-5Dietrich, T., Sharma, P., Walter, C., Weston, P., & Beck, J. (2013). The epidemiological evidence behind the association between periodontitis and incident atherosclerotic cardiovascular disease. Journal of periodontology, 84, S70-S84. https://doi:10.1902/jop.2013.134008
  • Eroğlu, H. E., Tatlışen, A., & Özkul, Y. (2004). Mesane kanserli doku kültürlerindeki mikronükleus üzerine propolis ve mitomisin-c’nin etkileri. Erciyes Üniversitesi Sağlık Bilimleri Dergisi (E.Ü.Journal of Health Sciences) 13(2) 15-20
  • Farquharson, D., Butcher, J. P., & Culshaw, S. (2012). Periodontitis, Porphyromonas, and the pathogenesis of rheumatoid arthritis. Mucosal immünology, 5(2), 112–120. https://doi:10.1038/mi.2011.66
  • Graves, D. T., Kang, J., Andriankaja, O., Wada, K., & Rossa Jr, C. (2012). Animal models to study host-bacteria interactions involved in periodontitis. Periodontal Disease, 15, 117-132. https://doi: 10.1159/000329675
  • Hajishengallis, G., & Chavakis, T. (2021). Local and systemic mechanisms linking periodontal disease and inflammatory comorbidities. Nature Reviews Immunology, 1-15. https://doi.org/10.1038/ s41577-020-00488-6
  • Ikeno K, Ikeno T, Miyazawa C. Effects of propolis on dental caries in rats. Caries Research. 1991; 25(5):347-51. https://doi.org/10.1159/000261390
  • Koo, H., Gomes, B. P. F. A., Rosalen, P. L., Ambrosano, G. M. B., Park, Y. K., & Cury, J. A. (2000). In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Archives of oral biology, 45(2), 141-148. https://doi.org/10.1016/S0003-9969(99)00117-X
  • Koru, O., Toksoy, F., Acikel, C.H., Tunca, Y.M., Baysallar, M., Guclu, A.U. (2007). In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe. 13(3):140–5. https://doi.org/10.1016/j.anaerobe.2007.02.001
  • Kujumgiev, A., Tsvetkova, I., Serkedjieva, Y., Bankova, V., Christov, R., Popov, S.(1999). Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of ethnopharmacology. 64(3):235-40. https://doi.org/10.1016/S0378-8741(98)00131-7
  • Nishihara, R., Sugano, N., Takano, M., Shimada, T., Tanaka, H., Oka, S., & Ito, K. (2009). The effect of Porphyromonas gingivalis infection on cytokine levels in type 2 diabetic mice. Journal of periodontal research, 44(3), 305-310. doi:10.1111/j.1600-0765.2008.01130.x
  • Olsen, I. (2015). From the acta prize lecture 2014: The periodontal-systemic connection seen from a microbiological standpoint: Summary of the Acta Odontologica Scandinavia Price lecture 2014 presented at the meeting of the IADR/Pan European region in Dubrovnik, September 10–13. 2014. Acta Odontologica Scandinavica, 73(8), 563-568. https://doi.org/10.3109/00016357.2015.1007480
  • Orsi, R., Sforcin, J., Rall, V., Funari, S., Barbosa, L., Fernandes, J. (2005). Susceptibility profile of Salmonella against the antibacterial activity of propolis produced in two regions of Brazil. Journal of Venomous Animals and Toxins including Tropical Diseases, 11(2), 109-116.
  • Paraskevas, S., Huizinga, J. D., & Loos, B. G. (2008). A systematic review and meta‐analyses on C‐reactive protein in relation to periodontitis. Journal of clinical periodontology, 35(4), 277-290. https://doi: 10.1111/j.1600-051X.2007.01173.x
  • Popova, M., Silici, S., Kaftanoglu, O., & Bankova, V. (2005). Antibacterial activity of Turkish propolis and its qualitative and quantitative chemical composition. Phytomedicine, 12(3), 221-228. https://doi.org/10.1016/j.phymed.2003.09.007
  • Poppe, B. & Michaelis, H. (1986). Results of a twice- yearly controlled oral hygiene activity using a propolis-containing toothpaste (double-blind study). Stomatologie der DDR, 36(4), 195-203.
  • Preshaw, P.M. & Taylor, J.J.(2011). How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol, 38(11), 60-84. https://doi: 10.1111/j.1600-051X.2010.01671.x
  • Ryder, M. I. (2010). Comparison of neutrophil functions in aggressive and chronic periodontitis. Periodontology 2000, 53(1), 124–137.
  • Seidel V, Peyfoon E, Watson DG, Fearnley J. Comparative study of the antibacterial activity of propolis from different geographical and climatic zones. Phytotherapy Research. 2008;22(9):1256-63. DOI: 10.1002/ptr.2480
  • Singh, M., Briones, M., & O’Hagan, D. T. (2001). A novel bioadhesive intranasal delivery system for inactivated influenza vaccines. Journal of controlled release, 70(3), 267-276. https://doi.org/10.1016/S0168-3659(00)00330-8
  • Skaba, D., Morawiec, T., Tanasiewicz, M., Mertas, A., Bobela, E., Szliszka, E., Król, W. (2013). Influence of the toothpaste with Brazilian ethanol extract propolis on oral cavity health. Evidence-Based Complementary and Alternative Medicine. Article ID 215391 | https://doi.org/10.1155/2013/215391
  • Struillou, X., Boutigny, H., Soueidan, A., & Layrolle, P. (2010). Experimental animal models in periodontology: a review. The open dentistry journal, 4, 37. 10.2174/1874210601004010037
  • Takano, M., Nishihara, R., Sugano, N., Matsumoto, K., Yamada, Y., Takane, M., ... & Ito, K. (2010). The effect of systemic anti-tumour necrosis factor-alpha treatment on Porphyromonas gingivalis infection in type 2 diabetic mice. Archives of oral biology, 55(5), 379-384. https://doi.org/10.1016/j.archoralbio.2010.03.004
  • Velazquez C, Navarro M, Acosta A, Angulo A, Dominguez Z, Robles R, et al. Antibacterial and free-radical scavenging activities of Sonoran propolis. Journal of Applied Microbiology. 2007; 103(5):1747-56. doi:10.1111/j.1365-2672.2007.03409.x
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Research Article
Yazarlar

Ömer Ebrem 0000-0001-8042-5954

Pınar Alkım Ulutaş 0000-0002-2447-3027

Proje Numarası VTF-21025
Yayımlanma Tarihi 28 Aralık 2022
Gönderilme Tarihi 7 Ekim 2022
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Ebrem, Ö., & Ulutaş, P. A. (2022). Effects of Mucoadhesive Gel Containing Propolis on Some Biochemical and Hematologic Parameters in Rats With Experimental Periodontitis. Animal Health Production and Hygiene, 11(2), 44-49. https://doi.org/10.53913/aduveterinary.1185773