Çözüm sonu teke spermasına ATP ilavesinin sperma parametreleri üzerine etkisi

Öz

Çözüm sonrası teke spermatozoonlarına farklı doz seviyelerinde ATP takviyesinin motilite ve diğer sperm parametreleri üzerindeki etkisi 4 saatlik inkübasyon süresince değerlendirildi. Ejekülat, yumurta sarısı-glikoz-gliserol-tris-sitrik asit sulandırıcısı kullanılarak 0.25 ml payetlerde donduruldu. Su banyosunda 30 saniye süreyle 37°C'de payetler çözüldü ve sperm hücreleri, sulandırıcı içeriklerinin uzaklaştırılmasının ardından 0 (kontrol), 2, 4 veya 8 mM ATP eklenmeden önce tris-sitrik asit-glikoz çözeltisi içerisinde yeniden seyreltildi. Spermatolojik parametrelerin büyük çoğunluğu ATP takviyesinden (P<0.05) olumlu biçimde etkilenmiştir. ATP takviyesi (2-4 mM), orta hızda progresif sperm alt popülasyonunun yüzdesini artırarak toplam progresif hareketliliği yükseltti (P<0.05). Ancak ATP takviyesi ALH, VAP, VCL ve VSL değerlerini artırmadı. Buna karşılık, ATP' nin aşırı doz seviyeleri (8 mM), ALH, VAP, VCL ve VSL dahil olmak üzere hız parametrelerinin çoğunu (P<0.001) baskıladı. Ölü-canlı, akrozom reaksiyonu oranı ve mitokondriyal aktivite doz seviyelerine bakılmaksızın ATP takviyesinden etkilenmedi (P>0.05). Sonuç olarak, çözüm sonrası teke spermatozoasına ATP takviyesi (2-4 mM), ALH, VCL, VAP ve VSL dahil olmak üzere kinematik sperm parametrelerinin çoğu üzerinde belirgin bir etki olmaksızın inkübasyon periyodu boyunca progresif motiliteyi geliştirdi (P<0.05). ATP konsantrasyonunun 8 mM seviyesine çıkarılması motilite parametrelerini önemli ölçüde düşürdü. (P<0.01). ATP takviyesinin, teke spermatozoasında kriyoprezervasyondan kaynaklanan azalmış motilite problemini en azından kısmen düzeltebileceği sonucuna varıldı.

Anahtar Kelimeler

• akrozom reaksiyonu
• ATP
• Çözüm sonu
• Teke sperması
• motilite

Effect of ATP Addition to Goat Semen Post Thaw on Semen Parameters

Öz

In post-thaw goat spermatozoa the impact of ATP supplementation at different dose levels on motility and the other sperm parameters was evaluated during 4 hours of incubation. The ejaculate was frozen in 0.25ml straws by using egg yolk-glucose-glycerol-tris-citric acid extender. Thawing was achieved at 37°C for 30 second in a water bath and sperm cells were rediluted, following the removal of the extender, in tris-citric acid-glucose solution before adding 0 (control), 2, 4 or 8 mM of ATP. Eight of 10 parameters that were examined by the computer assisted sperm analyzer has been influenced by ATP supplementation (P<0.05) at dose dependent manner. ATP supplementation (2-4mM) increased (P<0.05) progressive motility by enhancing the percentage of medium progressive sperm subpopulation (P<0.05). However, ATP supplementation did not increase ALH, VAP, VCL and VSL values. In contrast, excessive dose levels of ATP (8mM) depressed most of the velocity parameters (P<0.001) including ALH, VAP, VCL and VSL. The sperm parameters other than motility, live and acrosome reacted sperm rate and mitochondrial activity were not influenced (P>0.05) by ATP supplementation irrespective to the dose levels. As a result, ATP supplementation (2-4mM) to post-thaw goat spermatozoa improved progressive motility during the incubation (P<0.05) without any prominent influence on most of the kinematic sperm parameters including ALH, VCL, VAP and VSL. Further increase the ATP concentrations to 8mM suppressed motility parameters significantly (P<0.01). It was concluded that ATP supplementation might restore reduced motility problem, at least partly, in goat spermatozoa resulting from cryopreservation.

Anahtar Kelimeler

• acrosome reaction
• ATP
• goat spermatozoa
• motility
• post-thaw

Kaynakça

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