Identification of Pathogen Bacteria from Bovine Mastitis In Yozgat Province And Determination of Antimicrobial Susceptibility

Öz

Backround/Aim: The aim of this study was to isolate and identify the bacterial microorganisms causing subclinical mastitis and to determine their susceptibility and resistant to antibiotics in Yozgat province and districts. Results and Conclusion: In isolated milk samples 174 with bacterial growth, Gram positive 63 and Gram negative 111 microorganisms were respectively isolated at 36.21% and 63.79%. A total of 238 bovine milk samples with mastitis were used and the isolated agents were identified as Aeromonas hydrophila 30 17.24% , Citrobacter diversus 4 2.29% , Citrobacter freundii 2 1.14% , Corynebacterium sp. 24 13.79% , Escherichia coli 18 10.34% , Enterobacter cloacae 2 1.14% , Flavobacter sp. 3 1.72% , Lactobacillus sp. 21 12.06% , Pasteurella pneumonia 2 1.14% , Plesiomonas shigelloides 11 6.32% , Providencia sp. 2 1.14% , Salmonella sp. 2 1.14% , Serratia liquefacians 2 1.14% , Shigella sp. 18 10.34% , Vibrio cholerae 6 3.45% , Yersinia enterocolitica 1 0.57% , coagulase-negative staphylococcus CNS 18 10.34% and Staphylococcus aureus 8 4.59% . Aeromonas hydrophila 17.24% was the most isolated agent from milk samples. Antibiotic resistance was evaluated in all isolated pathogens and the highest resistance was observed against erythromycin 63/174, 36.20% followed by penicillin G 53/174, 30.45% , amoxicillin 38/174, 21.83% and gentamicin 23/174, 13.21% .

Anahtar Kelimeler

• Antibiotic Susceptibility Test,Cow,Identification,Mastitis.

Yozgat İlinde Bulunan Sığır Mastitislerinden Patojen Bakterilerin İdentifikasyonu Ve Antibiyotiklere Duyarlılıklarının Belirlenmesi

Öz

Özbilgi/Amaç: Bu çalışmada Yozgat ili merkez ve ilçelerinde bulunan ve mastitis enfeksiyonlarına neden olan bakteriyel etkenlerin izolasyonu ve identifikasyonu ile bu etkenlere karşı etkili ve dirençli olan antibiyotiklerin belirlenmesi amacıyla toplam 238 adet mastitisli sığır süt örneği kullanılmıştır. Bulgular ve Sonuç: Bakteriyel üremenin meydana geldiği süt örneklerinde 174 %36.21 oranında Gram pozitif 63 ve %63.79 oranında Gram negatif 111 mikroorganizma izole edilmiştir. İzole edilen etkenler Aeromonas hydrophila 30 %17.24 , Citrobacter diversus 4 %2.29 , Citrobacter freundii 2 %1.14 , Corynebacterium sp. 24 %13.79 , Escherichia coli 18 %10.34 , Enterobacter cloacae 2 %1.14 , Flavobacter sp. 3 %1.72 , Lactobacillus sp. 21 %12.06 , Pasteurella pneumonia 2 %1.14 , Plesiomonas shigelloides 11 %6.32 , Providencia sp. 2 %1.14 , Salmonella sp. 2 %1.14 , Serratia liquefacians 2 %1.14 , Shigella sp. 18 %10.34 , Vibrio cholerae 6 %3.45 , Yersinia enterocolitica 1 %0.57 , Koagülaz Negatif Staphylococcus 18 %10.34 ve Staphylococcus aureus 8 %4.59 olarak tespit edilmiştir. Süt örneklerinde en çok izolasyonu yapılan tür %17.24 oranla Aeromonas hydrophila olmuştur. Tüm izole edilen patojenlerin antibiyotik direnç durumu değerlendirildiğinde en fazla direnç eritromisin’de 63/174, %36.20 görülmüş ve penisilin G 53/174, %30.45 , amoksisilin 38/174, %21.83 ve gentamisin 23/174, %13.21 ’nin takip ettiği gözlenmiştir.

Anahtar Kelimeler

• Antibiyotik Duyarlılık Test,İdentifikasyon,İnek,Mastitis.

Kaynakça

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