Forelimb Lameness in Merino and Akkaraman Sheep Associated with Naturally Occuring Brucella melitensis Infection

Öz

Background/Aim: Brucellosis has been still been recognized as an important infectious disease among ruminants worldwide. Disease reports in recent years indicated an elevation in the number of outbreaks in sheep. The objective of the present study was to describe forelimb lameness observed in a mixed Merino and Akkaraman sheep flock associated with Brucella melitensis infection. Material and Method: A descriptive case control study was conducted on 107 sheep in a sheep flock consisting 450 mixed Merino and Akkaraman during the period of April-September 2012. The brucellosis status of the present flock was established through serological testing of sera samples by use of Rose Bengal Plate RBPT , serum agglutination SAT tests and polymerase chain reaction PCR applied in series. Results and Conclusion: Out of those 107 sera, RBPT revelaed 93 86% positivity, and out of the latter animals SAT recorded 60 65% positive samples with titers equal or above 1/40, whereas PCR recorded 83 89% positivity. Seventeen out of 107 cases tested showed forelimb lameness, interestingly all those animals were detected to have forelimb lameness with SAT titers 1/40 and > and PCR positivity. Clinical, epizootiological and microbiological aspects of Brucella infection were described in the present article.

Anahtar Kelimeler

• forelimb,lameness,Merino,Akkaraman,sheep,Brucella melitensis

Merinos ve Akkaraman Koyunlarında Doğal Brucella melitensis Enfeksiyonuna Bağlı Ön Bacak Topallığı

Öz

Özbilgi/Amaç: Brusellozis, halen geviş getirenlerin dünya çapında önemli bir enfeksiyöz hastalığı olarak kabul edilmektedir. Son yıllarda hastalığa ilişkin raporlar koyun salgınlarının sayısında bir artışı göstermektedir. Bu çalışmada karışık Merinos ve Akkaraman koyun sürüsünde Brucella melitensis enfeksiyonuna bağlı şekillenen ön bacak topallığının tanımlanması amaçlandı. Materyal ve Metot: Nisan-Eylül 2012 ayları arasında 450 başlık karışık Merinos ve Akkaraman koyun sürüsünde yapılan tanımlayıcı vaka kontrol çalışması 107 koyun üzerinde gerçekleştirildi. Sürüde brusellozis durumunun belirlenmesi amacıyla serum örneklerinde sırasıyla Rose Bengal Plate RBPT , serum aglütinasyon SAT testleri ve polimeraz zincir reaksiyonu PCR uygulandı. Bulgular ve Sonuç: Yüzyedi serumdan 93’ü %86 RBPT pozitif tespit edildi ve bu hayvanlardan yapılan testlerde, titresi 1/40 ve üzerinde olan 60 %65 örnek SAT pozitif bulunurken, 83 %89 örnek ise PCR pozitif olarak kaydedildi. 107 koyundan 17’sinde ön bacak topallığı gözlendi ve ilginç olarak bu hayvanların tümünün 1/40 ve üzeri SAT titrelerine sahip olduğu ve PCR pozitif oldukları tespit edildi. Bu makalede Brucella enfeksiyonu klinik, epizootiyolojik ve mikrobiyolojik yönden tanımlandı.

Anahtar Kelimeler

• ön bacak,topallık,Merinos,Akkaraman,koyun,Brucella melitensis

Kaynakça

1. Adams OR (1974). In: Lameness in Horses, 3rd, Lea and Febiger (Eds): Philadelphia, pp. 4–32.
2. Alton GG (1990). Brucella melitensis. In: Animal Brucellosis, Nielsen.K and Duncan JR (Eds): CRC Pres, Boston, pp. 205-215.
3. Baily GG, Krahn JB, Drasar BS and Stocker NG (1997). Detection of Bru- cella abortus and Brucella melitensis by DNA amplification. The American Journal of Tropical Medicine and Hygiene, 95, 271-275.
4. Blood DC and Radostits OM (1990). In: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 10th Ed., Mackay’s Kent, Bri- tain. pp: 449–487.
5. Bokko BP and Chaudhari SUR (2001). Prevalence of lameness in sheep in the north east region of Nigeria. International Journal of Agricul- ture and Biology, 3 (4): 519 – 521.
6. Boschiroli ML, Foulongne V and O’Callaghan D (2001). Brucellosis: a worldwide zoonosis. Current Opinion in Microbiology, 4, 58-64.
7. Bosilkovski M, Krteva L, Caparoska S and Dimzova M (2004). Osteoarti- cular involvement in brucellosis: study of 196 cases in the Republic of Macedonia. Croatian Medical Journal, 45, 727-733.
8. Bricker BJ (2002). PCR as a diagnostic tool for brucellosis. Veterinary Microbiology, 90, 435-446.

9. Bricker BJ and Halling SM (1994). Differentiation of Brucella abortus bv. 1, 2, and 4 Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. Journal of Clinical Microbiology, 32, 2660-2666, 1994.
10. Chadda VS., Soni PK., Gupta A., Gupta BK., Chadda S., Nayak KC. (2004). Incidence of brucellosis in arthritis and chronic low back pain in high risk group. Journal of Association of Physicians of India; 52: 338.
11. Colmenero Jde D, Queipo-Ortuño MI, Maria Reguera J, Angel Sua- rez-Muñoz M, Martín-Carballino S and Morata P (2002). Chronic hepatosplenic abscesses in Brucellosis. Clinico-therapeutic features and molecular diagnostic approach. Diagnostic Microbiology and Infectious Disease, 42, 159-67.
12. Corbel MJ, Stuart FA, Brewer RA, Jeffrey M and Bradley R (1989). Arth- ropathy associated with Brucella abortus strain 19 vaccination in cattle. I. Examination of field cases. British Veterinary Journal, 145(4): 337-346.
13. Corbel MJ (1997). Brucellosis: an overview, Emerging Infectious Dise- ases, 3, 213–221.
14. DelVecchio VG, Kapatral V, Redkar RJ, Patra G, Mujer C, Los T, Ivanova N, Anderson I, Bhattacharyya A, Lykidis A, Reznik G, Jablonski L, Larsen N, D’Souza M, Bernal A, Mazur M, Goltsman E, Selkov E, Elzer PH, Hagius S, O’Callaghan D, Letesson JJ, Haselkorn R, Kyrpides N and Overbeek R (2002). The genome sequence of the facultative intra- cellular pathogen Brucella melitensis. Proceedings of the National Academy of Sciences of the United States of America, 99, 443-448.
15. Devendra C and McLorey GB (1982). In: A Textbook of sheep and Goat Production in the Tropics. International Tropical Agriculture Series. Longman, London. Pp. 352–353.
16. Egwu GO, Adamu SS, Ameh JA, Onyeyili PA, Abana PS, Chaudhari SUR and Rabo JS (1994) Retrospective, clinic pathological and microbi- ological studies of interdigital pouch lameness in sheep in an arid zone of Nigeria. Bulletin of Animal Health and Production in Africa, 42, 5–11.
17. Glynn MK and Lynn T V (2008). Brucellosis. Journal of the American Veterinary Medical Association, 233, 900–908.
18. Gotuzzo E, Seas C, Guerra JG, Carrillo C, Bocanegra TS, Calvo A, Cas- tañeda O and Alarcón GS (1987). Brucellar arthritis: a study of 39 Peruvian families, Annals of the Rheumatic Diseases, 46, 506-509.
19. Gupta VK, Deepak K, Verma P K, Singh SV and Vihan VS (2006). Poly- merase Chain Reaction (PCR) for Detection of Brucella melitensis in goat milk. Small Ruminant Research, 65, 79-84.
20. Güler L, Gündüz K and Ok Ü (2003). Comparison of polymerase cha- in reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Veterinary Microbiology, 93, 53-61.
21. Harris DJ, Hiburt CD and Anderson GA (1988). Incidence, cost and fac- tors associated with foot lameness in dairy cattle in South Victoria. Australian Veterinary Journal, 65, 171.
22. Hartigan P (1997). Human brucellosis: epidemiology and clinical mani- festations. Irish Veterinary Journal, 50, 179-80.
23. Kater JC and Hartley WJ (1963). Lameness in rams following vaccination against Brucella ovis infection. New Zealand Veterinary Journal, 11, 3, 65-67.
24. Kim J and Breur GJ (2008). Temporospatial and kinetic characteristics of sheep walking on a pressure sensing walkway. Canadian Journal of Veterinary Research, 72, 1, 50-55.
25. Koneman E (2006) Chapter 9: Brucella species. In: Koneman’s Color At- las and Textbook of Diagnostic Microbiology, 6th Edit., Winn W, Al- len S, Janda W, Koneman E, Procop G, Schreckenberger P and Wood G (Eds.), Lippincott Williams and Wilkins Publication, United States of America, pp. 482-491.
26. Leal-Klevezas DS, Martinez-Vazquez IO, Lopezmerino A and Mart- nez-Sorano JP (1995) Singlestep PCR for detection of Brucella spp. from blood and milk of infected animals. Journal of Clinical Micro- biology, 33, 3087-3090.
27. Lopes LB, Nicolino R, Haddad JPA (2010) Brucellosis - Risk Factors and Prevalence: A Review. The Open Veterinary Science Journal, 4, 72- 84.
28. Manthur BG, Peerapur BV, Shetty SKV, Purohit SN, Shrikant B and Sa- rayu L (1994) Brucellosis among the patients attending B.L.D.E.A’s medical college hospital, Bijapur- a serological and cultural study. Indian Journal of Medical Microbiology,12, 1, 35-38.
29. Megid J, Mathias LA and Robles CA (2010) Clinical Manifestations of Brucellosis in Domestic Animals and Humans. The Open Veterinary Science Journal, 4, 119-126
30. Öngör H, Muz A and Çetinkaya B (2001) Atık yapmış koyunlarda bru- sellosis teşhisinde ELISA ile diğer serolojik testlerin karşılaştırılması. Turkish Journal of Veterinary and Animal Science, 25, 21-26.
31. Pourbagher A, Pourbagher MA, Savas L, Turunc T, Demiroglu YZ, Erol I and Yalcintas D (2006) Epidemiologic, clinical and imaging findigns in brucellosis patients with osteoarticular involvement. American Journal of Roentgenology, 187, 873-880.
32. Renukaradhya GJ, Isloor S and Rajasekhar M (2002) Epidemiology, zoo- notic aspects, vaccination and control/eradication of brucellosis in India. Veterinary Microbiology. 90, 183–195.
33. Wallach JC, Samartino LE, Efron A and Baldi PC (1998) Human infecti- on by Brucella melitensis: An outbreak attributed to contact with infected goats. FEMS Immunology and Medical Microbiology, 19, 315-321.
34. Wernaers P and Handelberg F (2007) Brucellar arthritis of the knee: A case report with delayed diagnosis. Acta Orthopaedica Belgica, 73, 795-798.
35. West DM, Johnstone AC, Bruere AN and Chapman HM (1978) Epiphy- sitis in rams following vaccination against Brucella ovis infection. New Zealand Veterinary Journal, 26, 5, 133-134.
36. Zaks N, Sukenik S, Alkan M, Flusser D, Neumann L and Buskila D (1995) Musculoskeletal manifestations of brucellosis :A study of 90 cases in Israel. Seminars in Arthritis and Rheumatism, 25, 97-102.
37. Zamani A, Kooraki S, Mohazab RA, Zamani N, Matloob R, Hayatbakhsh MR and Raeeskarami SR (2011) Epidemiological and clinical featu- res of Brucella arthritis in 24 children. Annals of Saudi Medicine, 31(3): 270-273.