Efficacy of Seed Storage Proteins of Cereal Grains on Aspergillus and Fusarium spp.

Öz

The seed storage proteins contain almost half of the total proteins in mature cereal grains. They provide a great significance of nutritional value for human and livestock animals. Moreover, several other seed storage proteins possessing antifungal activity are characterized in cereal grains. In this study, antifungal activities of seed storage protein extracts from various cultivated and wild cereal species including Triticum durum, T. aestivum, Hordeum vulgare, Secale cereale, Triticale, Oryza sativa, Avena sativa, A. sterilis, and A. fatua, were examined on two plant pathogen fungi Aspergillus spp., Fusarium spp. by agar well diffusion assay. Results indicated that all protein extracts had various levels of antifungal activity; however extract of A. fatua, a wild type of Avena, had a superior antifungal activity on both Aspergillus and Fusarium spp. compared to the others. Extract of S. cereale ranged the second highest species against both fungi. In addition, extracts from Trakya O. sativa , Seydişehir A. sativa and Tatlıcak-97 Triticale had a strong fungal inhibitory potential against Fusarium spp. As a result, protein extract from A. fatua may be considered as a food preservative against Aspergillus and Fusarium spp. in practice and require further identification for its components.

Anahtar Kelimeler

• Antifungal activity,Cereal grains,Seed storage proteins

Tahıllardaki Tohum Depo Proteinlerinin Aspergillus ve Fusarium spp. Üzerine Etkinliği

Öz

Tohum depo proteinleri, tahıllardaki toplam proteinlerin takriben yarısını oluşturur. Bunlar insan ve çiftlik hayvanlarının beslenmesinde önemli bir yere sahiptir. Ayrıca tahıllardaki diğer tohum depo proteinlerinin de antifungal aktiviteye sahip olduğu bilinmektedir. Bu çalışmada, kültüre edilmiş ve yabani türlerden oluşan Triticum durum, T. aestivum, Hordeum vulgare, Secale cereale, Triticale, Oryza sativa, Avena sativa, A. sterilis, ve A. fatua tahıllarında tohum depo proteinleri özütlerinin antifungal aktiviteleri agar well difüzyon deneyiyle iki farklı bitki patojen mantar’ına Aspergillus spp., Fusarium spp. karşı incelenmiştir. Sonuçlar, bütün tahıl protein özütlerinin çeşitli düzeylerde antifungal aktiviteye sahip olduğunu göstermiştir. Ancak, yabani türde olan A. fatua özütleri diğer türlerle kıyaslandığında Aspergillus ve Fusarium spp. üzerinde üst düzey bir antifungal aktiviteye sahip olmuştur. İkinci sırada ise S. cereale özütleri her iki mantar’a karşı etkili olmuştur. Buna ilaveten, Trakya O. sativa , Seydişehir A. sativa ve Tatlıcak-97 Triticale özütleri ise Fusarium spp.’ye karşı güçlü bir mantar yok edici potansiyele sahip olmuştur. Sonuç olarak, A. fatua protein özütlerinin uygulamada gıda koruyucu ajan olarak Aspergillus ve Fusarium spp.’lere karşı kullanımı düşünülebilir. Ayrıca bu protein özütlerinin diğer bileşenlerinin de ileriki çalışmalarda araştırılması düşünülebilir

Anahtar Kelimeler

• Antifungal aktivite,Tahıllar,Tohum depo proteinleri

Kaynakça

1. Abd-Alla HI, Shaaban M, Shaaban KA, Abu-Gabal NS, Shalaby NMM and Laatsch H (2009). New bioactive compounds from Aloe hijazensis. Natural Product Research, 23, 1035-1049.
2. Bal EBB and Bay S (2010). Genetic analysis of Turkish rice varieties (Oryza sativa L.) using seed storage proteins and RAPD markers. European Food Research and Technology, 230, 609-617.
3. Dykes L and Rooney LW (2007). Phenolic compounds in cereal grains and their health benefits. Cereal Food World, 52, 105-111.
4. Gallagher RS, Ananth R, Granger K, Bradley B, Anderson JV and Fuerst EP (2010). Phenolic and short-chained aliphatic organic acid constituents of wild oat (Avena fatua L.) seeds. Journal of Agricultural and Food Chemistry, 58, 218-225.
5. Huynh QK, Hironaka CM, Levine EB, Smith CE, Borgmeyer JR and Shah DM (1992). Antifungal proteins from plants: purification, molecular cloning, and antifungal properties of chitinases from maize seed. The Journal of Biological Chemistry, 267, 6635-6640.
6. Jayasuriya KE, Whesunderaz RLC and Deraniyagala SA (2003). Isolation of anti-fungal phenolic compounds from petioles of two Hevea brasiliensis (rubber) genotypes and their effect on Phytophthora meadii. Annals of Applied Biology, 142, 63-69.
7. Leah R, Tommerup H, Svendsen I and Mundy J (1991). Biochemical and molecular characterization of three barley seed proteins with antifungal properties. The Journal of Biological Chemistry, 266, 1564-1573.
8. Leah R, Skriver K, Knudsen S, Ruud-Hansen J, Raikhel NV and Mundy J (1994). Identification of an enhancer/silencer sequence directing the aleurone-specific expression of a barley chitinase gene. Plant Journal, 6, 579-589.

9. Liu B, Lu Y, Xin Z and Zhang Z (2009). Identification and antifungal assay of a wheat β-1,3-glucanase. Biotechnology Letters, 31, 1005-1010.
10. Loon LC and Strien EA (1999). The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiological and Molecular Plant Pathology, 55, 85-97.
11. Macias FA, Marin D, Oliveros-Bastidas A, Chinchilla N, Simonet AM and Molinillo JMG (2006). Isolation and synthesis of allelochemicals from gramineae: benzoxazinones and related compounds. Journal of Agricultural and Food Chemistry, 54, 991-1000.
12. Macias FA, Chinchilla N, Arroyo E, Varela RM, Molinillo JMG and Marin D (2010). Multifunctionalised benzoxazinones in the systems Oryza sativa-Echinochloa crus-galli and Triticum aestivum-Avena fatua as natural-product-based herbicide leads. Pest Management Science, 66, 1137-1147.
13. Osbourn AE, Clarke BR, Lunness P, Scott PR and Daniels MJ (1994). An oat species lacking avenacin is susceptible to infection by Gaeumannomyces graminis var. tritici. Physiological and Molecular Plant Pathology, 45, 457-467.
14. Papadopoulou K, Melton RE, Leggett M, Daniels MJ and Osbourn AE (1999). Compromised disease resistance in saponin-deficient plants. Proceedings of the National Academy of Sciences of the United States of America, 96, 12923-12928.
15. Sancho AI, Gillabert M, Tapp H, Shewry PR, Skeggs PK and Mills ENC (2008). Effect of environmental stress during grain filling on the soluble proteome of wheat (Triticum aestivum) dough liquor. Journal of Agricultural and Food Chemistry, 56, 5386-5393.
16. Schillinger U and Lucke FK (1989). Antibacterial activity of Lactobacillus sake isolated from meat. Applied and Environmental Microbiology, 55, 1901-1906.
17. Shewry PR and Halford NG (2002). Cereal seed storage proteins: structures, properties and role in grain utilization. Journal of Experimental Botany, 53, 947-958.
18. Sorensen HP, Madsen LS, Petersen J, Andersen JT, Hansen AM and Beck HC (2010). Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase. Applied Biochemistry and Biotechnology, 160, 1573-1584.
19. Taira T, Ohnuma T, Yamagami T, Aso Y, Ishiguro M and Ishihara M (2002). Antifungal activity of rye (Secale cereale) seed chitinases: the different binding manner of class I and class II chitinases to the fungal cell walls. Bioscience, Biotechnology, and Biochemistry, 66, 970-977.
20. Turkusay H and Onogur E (1998). Studies on antifungal effects of some plant extracts in vitro. Turkish Journal of Agriculture and Forestry, 22, 267-271.