Escherichia coli O157 and O157:H7 in Raw Cow Milk

Yıl 2017, Cilt: 6 Sayı: 1, 481 - 486, 01.01.2017

Gökhan İnat Belgin Sırıken Şebnem Pamuk

Öz

Background/Aim: Infection with Escherichia coli E. coli O157 and O157:H7 is a significant health concern in a growing number of regions around the world. Dairy cattle have been regarded as primary host of the bacteria and raw cow milk or milk products carry a potential risk for the bacteria. Material and Methods:Therefore, this study was conducted in a Turkish province to investigate the presence of E. coli O157 and O157:H7 strains and to detect the presence of the stx1, stx2 genes and present of intimin eaeA gene in the isolates obtained from 150 raw cow milk samples using enrichment-based immunomagnetic separation IMS technique and multiplex PCR assay, respectively. Results and Conclusion: As a result, E. coli O157 was detected in one 0.66% out of 150 raw cow milk samples, and the number of isolates obtained from one raw milk sample was two. Then, these two isolates were analyzed for being E. coli O157:H7. According to analyses result, the two isolates were identified as E. coli O157:H7. While the eaeA gene alone was detected in the one isolates, none of the stx1 and stx2 genes were detected in the isolates. Due to the higher possibility for contamination of milk at dairy farms with E. coli O157 or O157:H7, consumption of raw milk should be avoided.

Anahtar Kelimeler

E. coli O157, intimin eaeA, O157:H7, raw milk, stx1, stx2

Çiğ İnek Sütlerinde Escherichia coli O157 ve O157:H7 Varlığı

Öz

Özbilgi/Amaç:Escherichia coli E. coli O157 ve O157:H7 dünyanın hemen her bölgesinde görülen ve özellikle çocuklar olmak üzere, her yaştan insan için önemli sağlık problemlerine yol açan en önemli gıda patojenlerindendir. Ruminantlar, özellikle de süt sığırları bu bakterinin ana konakçısıdır ve bağırsak gaitaları ile çevreyi kontamine edebilmektedir. Bu nedenle çiğ inek sütü ve süt ürünleri bu bakteri için potansiyel bir risk taşımaktadır. Materyal ve Metod : Bu çalışma Samsun ili ve çevre köylerinde yetiştirilen ineklerden alınan 150 çiğ süt örneğinde, zenginleştirme-temelli immunomanyetik seperasyon IMS tekniği ve multipleks PZR yöntemlerini kullanarak sırasıyla E. coli O157 ve O157:H7 varlıklarının belirlenmesi ve elde edilen izolatlarda stx1, stx2 ve intimin eaeA gen varlıklarını saptamak için yapıldı. Bulgular ve Sonuç: Sonuç olarak, analiz edilen 150 çiğ süt örneğinin sadece 1’inde % 0.66 E. coli O157 izole edildi. Elde edilen izolat sayısı ise iki idi. Daha sonra bu iki izolat O157:H7 yönünden analiz edildi ve analiz sonucunda bu iki izolat E. coli O157:H7 olarak identifiye edildi. İzolatlarda stx1, stx2 ve intimin eaeA gen varlığı belirleme çalışmaları sonucunda; intimin eaeA varlığı sadece bir izolatta saptanırken, stx1ve stx2 gen hiçbir izolatta saptanamadı. Sütçü sığır çiftliklerinde sütün E. coli O157 ve O157:H7 ile kontamine olabilme olasılığının diğer çiftliklere göre daha yüksek oluşu nedeniyle, çiğ süt tüketiminden kaçınılmalıdır.

Anahtar Kelimeler

Çiğ süt, E. coli O157, O157:H7, intimin eaeA, stx1, stx2

Kaynakça

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