The Identification of Indicator Bacteria and Determination of Their Antibiotic Susceptibility in Laying Hens in Aydın Province

Yıl 2015, Cilt: 4 Sayı: 2, 411 - 414, 01.06.2015

Gökhan Ege Osman Kaya

Öz

Background/Aim: The aim of this study was determination of isolation and antibiotic susceptibility of E. coli and Enterococcus species from 200 cloacal samples which were collected from hen laying poultry houses found in Aydın province. Material and Method: The samples were brought to The Routine Diagnostic Laboratory of Microbiology Department, Veterinary Faculty, Adnan Menderes University under cold chain. Then was allowed to incubate for 24 hours at 37 ºC. After that, Gram positive and Gram negative bacteria are separated by using Gram Staining and isolated bacterias were identified based on biochemical assays. Disc diffusion method was used for the detection of antibiotic susceptibilities. Results and Conclusion: A total of 21 10 % E. faecalis, 24 12 % E. faecium and 40 20 % E. coli isolation and identification were made from a total, upon biochemical properties and carbonhydrate fermentation tests. According to antibiogram test results it was revealed that, E. faecalis was highly resistant to tetracyclin % 78 and kanamycin % 90 , E faecium was was highly resistant to kanamycin % 67 and E. coli was highly resistant to penicilin novobiocin % 100 . Besides, it was revealed that E. faecalis was totally susceptible to penicilin novobiocin, amoxycillin clavulanic acid and gentamicin, E. faecium was totally susceptible to penicilin novobiocin and amoxycillin clavulanic acid, E. coli isolates were totally susceptible to florfenicol and gentamicin. Preference of penicillin-novobiocin, gentamicin and amoxycillin-clavulanic acid for E. faecalis infections, penicillin-novobiocin and amoxycillin-clavulanic acid for E. faecium infections and gentamicin for E. coli infections will allow the effective treatment for encountered bacterial infection.

Anahtar Kelimeler

E. coli, E. faecium, E. faecalis, bacteria isolation, antibiotic resistance

Aydın İlinde Bulunan Yumurtacı Tavuklarda İndikatör Bakterilerin İdentifikasyonu ve Antibiyotik Duyarlılıklarının Belirlenmesi

Öz

Özbilgi/Amaç: Bu araştırmada Aydın ilindeki yumurtacı tavuk kümeslerinden toplanan 200 adet kloakal svap örneğinden Escherichia coli ve Enterokok türleri ve antibiyotik duyarlılıklarının belirlenmesi amaçlanmıştır. Materyal ve Metot: Alınan örnekler soğuk zincir altında Adnan Menderes Üniversitesi Veteriner Fakültesi Mikrobiyoloji Rutin Teşhis laboratuarına getirilmiş ve besiyerlerine ekimleri yapılmıştır. Daha sonra 37 ºC ’de 24 saat inkubasyona bırakılmıştır. Yapılan Gram boyama sonrası Gram pozitif ve Gram negatif bakteriler ayırt edilmiş ve izole edilen bu bakterilerin biyokimyasal yöntemlere göre identifikasyonu gerçekleştirilmiştir. Antibiyotik duyarlılıklarının tespiti için disk difüzyon metodu kullanılmıştır. Bulgular ve Sonuç: Alınan örneklerden 21 % 10 ’inden E. faecalis, 24 % 12 ’ünden E. faecium ve 40 % 20 ’ından E. coli izolasyon ve identifikasyonu biyokimyasal ve karbonhidrat fermentasyon testlerine göre gerçekleştirilmiştir. Antibiyogram sonuçlarına göre E. faecalis’in tetrasiklin % 78 ve kanamisine % 90 , E. faecium’un kanamisine % 67 , E. coli’nin ise penisilin - novobiosine % 100 yüksek oranda dirençli olduğu tespit edilmiştir. Ayrıca, penisilin novobiosin, amoksisilin-klavulanik asit ve gentamisine E. faecalis’in, penisilin novobiosin ve amoksisilin-klavulanik asite E. faecium’un, florfenikol ve gentamisine karşı E. coli izolatlarının tamamının duyarlı oldukları sonucuna varılmıştır. E. faecalis enfeksiyonlarında penisilin-novobiosin, gentamisin ve amoksisilin- klavulanik asit, E. faecium enfeksiyonlarında penisilin-novobiosin ve amoksisilin- klavulanik asit, E. coli enfeksiyonlarda ise gentamisinin tercih edilmesi, karşılaşılan bakteriyel kökenli hastalıkların etkili bir şekilde tedavi edilmelerine olanak sağlayacaktır.

Anahtar Kelimeler

E. coli, E. faecium, E. faecalis, bakteri izolasyonu, antibiyotik direnci

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