The Relationship Between the Histological Quality of the Newly Formed Hoof Tissue and the Levels of Trace Elements in Blood Serum and Hoof Tissues During the Recovery Period of Some Hoof Diseases in Dairy Cows

Yıl 2015, Cilt: 4 Sayı: 1, 344 - 349, 01.01.2015

İbrahim Akın , Osman Sacit Görgül Murat Sarıerler

Öz

Backround: Lameness is considered in most important problems in dairy industry and caused to poor animal welfare and economic loses. White line disease, solea ulcer and heel erosion are common foot diseases among the dairy cows.Aim: The present study aimed to determine the levels of some trace elements in the blood serum and liver and hoof tissues in healthy dairy cows as well as in the blood serum and hoof tissues of those being in the diagnostic and recovery period of sole ulcer, heel erosion and white line diseases. The changes of hoof tissues during healing processe were also evaluated histopathologically. The treatment groups sole ulcer, heel erosion and white line diseases were consisted of 18 Holstein dairy cows. The blood and hoof tissue samples were taken at the beginning of the treatments day 0 and 15th, 30th, and 45th days of the treatments . Meanwhile, blood samples were colleceted before slaughtering procedure, and hoof and liver tissues were collected after slaughtering procedure from the six clinically healthy Holstein dairy cows control group in a slaghterhouse.Result and Conclusion: The mean concentrations of zinc, copper, iron and manganese in the blood serum of healthy animals were 28.1±3.3 µg/dl, 36.6±4.8 µg/dl, 31.8±3.5 µg/dl, and 2.6±0.1 µg/dl, respectively. The levels of these trace elements in the liver were also 205±12 ppm, 27±2 ppm, 541±274 ppm, and 2±1 ppm, respectively. Significant differences in the mean concentrations of zinc, copper, iron and manganese between control animals and treatment groups were found at the beginning day 0 and the 15th, 30th and 45th days of treatments. Furthermore, histopathological evaluations suggested that hoof tissue samples had increased cellularity and decreased keratinization during the treatment period. It was concluded that, the hoof diseases caused significant changes in the levels of trace elements in hoof tissues, and observed high levels of some trace elements would not inevitable indicate the healthy hoof tissue in cows. Besides, although animals are seen as clinically healed, the histological quality of the hoof tissue might be far below the expected levels.

Anahtar Kelimeler

Histopathology, cow, hoof, sole ulcer, white line diseases, heel erosion, trace minerals

Süt Sığırlarında Bazı Tırnak Hastalıklarının İyileşme Sürecinde Kan Serumu ve Tırnak Dokusu İz Element Düzeyleri ile Yeni Oluşan Tırnak Dokusunun Histolojik Kalitesi Arasındaki İlişki

Öz

Özbilgi: Topallık süt sığırcılığı endüstrisinde en önemli problemler arasında değerlendirilir; hayvan refahında düşmeye ve ekonomik kayıplara yol açar. Beyaz çizgi hastalığı, solea ülseri ve ökçe erozyonu süt sığırlarında sık karşılaşılan ayak hastalıklarındandır.Amaç: Sunulan çalışmada; sağlıklı hayvanların kan serumu, karaciğer dokusu ve tırnağın taban, ökçe ve beyaz çizgi bölgelerinde bulunan bazı iz element düzeyleri ve taban ülseri, ökçe erozyonu ve beyaz çizgi hastalıklarının tanı ve iyileşme süreçlerinde kan serumu ve tırnak dokusu iz element düzeyleri ile bu süreçte tırnak dokusundaki değişimlerin histopatolojik olarak ortaya konulması amaçlanmıştır. Tedavi gruplarını taban ülseri, ökçe erozyonu ve beyaz çizgi hastalığı oluşturan, toplam 18 baş Holstein ırkı sığırdan, tedavi öncesinde 0. gün ve tedavi sürecinde 15, 30 ve 45. günler kan ve lezyonlu bölgeden tırnak örnekleri, aynı zamanda kontrol grubunu oluşturmak amacı ile de mezbahada kesilen 6 baş sağlıklı Holstein ırkı sığırdan kesim öncesi kan, kesim sonrası tırnak ve karaciğer doku örnekleri alınmıştır.Bulgular ve Sonuç: Sağlıklı sığırlarda serum çinko 28,1±3,3 µg/dl, bakır 36,6±4,8 µg/dl, demir 31,8±3,5 µg/dl, manganez 2,6±0,1 µg/dl olarak; karaciğer çinko 205±12 ppm, bakır 27±2 ppm, demir 541±274 ppm, manganez 2±1 ppm olarak belirlenmiştir. Hastalık grupları kan ve tırnak dokusu çinko, bakır, demir ve manganezin 0, 15, 30 ve 45. gün düzeyleri ile kontrol grubu düzeyleri arasında istatistiki olarak önemli değişimler saptanmıştır. Histopatolojik değerlendirmelerde tırnak dokusundan alınan örneklerde iyileşme sürecine paralel olarak selüleritenin arttığı, keratinizasyonun ise azaldığı belirlenmiştir. Sonuç olarak, sığırlarda ayak hastalıklarına ilgili olarak tırnak dokusu iz element düzeylerinde önemli değişimler meydana geldiği, bazı iz element düzeylerinin yüksek bulunmasının tırnak dokusunun sağlıklı olduğuna işaret etmediği, klinik olarak iyileştiğine karar verilen tırnakların henüz yeterli histolojik kaliteye ulaşmadığı kanısına varılmıştır.

Anahtar Kelimeler

Histopatoloji, sığır, tırnak, solea ülseri, beyaz çizgi hastalığı, ökçe erozyonu, iz elementler.

Kaynakça

• Akın I, Belge A, Bardakcıoglu H, Sarıerler M, Kilic N (2013). Milk yield in dairy cows before and after treatment for foot diseases. In proceedings: International lameness Conference on Lameness in Ruminants, Bristol, UK, Pp 128-129.
• Akın İ (2004). İz Elementler ve Sığır Tırnak Hastalıkları. Veteriner Cerrahi Dergisi, 10(3-4), 54-61.
• Ballantine HT, Socha MT, Acan DPL, Tomlinson DJ, Johnson AB, Field AS, Shearer JK, Amstel SR (2002). Effects of Feeding Complexed Zinc, Manganese, Copper and Cobalt to Late Gestation and Lactating Dairy Cows on Claw Integrity, Reproduction and Lactating Performance. The Professional Animal Scientist, 18, 211-218.
• Blood DC, Hederson JA, Rodostitis OM (1979). Veterinary Medicine. A Textbook of the Disorders of Cattle, Sheep, Pigs and Horses. Fifth Edition, Bailliere Tindall, London.
• Budras KD, Mülling C, Anthauer K (2005). Membrane-coating Granules and the Intercelluler Cementing Substance (Membrane-coating Material) in the Epidermis in Different Regions of the Equine Hoof. Anatomia, Histologia, Embryologia, 34, 298-306.
• Harris CD, Hilburt GA, Anderson GA (1988). The Incidense, Cost and Factors Associated with Foot Lameness in Dairy Cattle in South- Western Victoria. Ausralian Veterinary Journal 65, 171-176.
• Hedges J, Blowey RW, Packington AJ, Callaghan CJO, Green LE (2001). A Longitudinal Field Trial Effect of Biotin on Lameness in Dairy Cows. Journal of Dairy Science, 84, 1969-1975.
• Huxley JN (2012). Lameness in cattle: An ongoing concern. The Veterinary Journal 193 (2012) 610–611.
• Kelly WR (1974). Veterinary Clinical Diagnosis. Second Edition, Bailliere Tindall, London.
• Kempson SA, Logue DN (1993). Ultrastuructural observations of hoof horn from dairy cows: the structure of the white line. Veterinary Record 132(20), 499-502.
• Kincaid RL, Lefebre LE, Cronrath JD, Socha MT, Johnson AB (2003). Effect of Dietary Cobalt Supplementation on Cobalt Metabolism and Performance of Dairy Cattle. Journal of Dairy Science, 86, 1405-1414.
• Kovacs AB, Szilagyi M (1973a). Data on Mineral Components of The Horny Part of The Foot of Cattle, Sheep and Swine. Acta Veterinaria Academia Scientarium Hungaricae, 23, 187-192.
• Kovacs AB, Szilagyi M (1973b). Mineral Contents of The Horn of The Foot of The Swine of Different Breed and Ages. Acta Veterinaria Academia Scientarium Hungaricae, 23, 241-246.
• Lischer C, Geyer H, Ossent P, Friedli K, Naf I (1998). Handbuch zur Pflege und Behandlung der Klauen beim Rind. Landwirtschaftliche Lehrmittelzentrale, Länggasse 79, CH-3052 Zollikofen. ISBN: 3-906679-62-4.
• McDowell LR (1992). Minerals in Animal and Human Nutrition. Academic Press, Inc. New York, USA. ISBN: 0-12-483369-1
• Mulling KW, Bragulla HH, Reese S, Budras KD, Steinberg W (1999). How Structures in Bovine Hoof Epidermis are Infuenced by Nutritional Factors. Anatomia, Histologia, Embryologia, 28, 103-108.
• Nocek JE, Johnson AB, Socha MT (2000). Digital Caracteristics in Commercial Dairy Herds Fed metal-Specific Amino Acid Complexes. Journal of Dairy Science, 83, 1553-1572.
• Nocek JE, Socha MT, Tomlinson DJ (2006). The Effect of Trace Mineral Fortication Level and Source on Performance of Dairy Cattle. Journal of Dairy Science, 89, 2679-2693.
• Randhawa SS, Dua, K, Singh ST, Randhawa CS (2014). Trace Mineral Status of Wall and Sole Portions of Healthy and Diseased hoofs in Cattle and Buffaloes. Indian Journal of Animal Science, 84(4), 417- 421.
• Reiling BA, Berger LL, Riskowski GL, Rompata RE (1992). Effects of Zinc Proteinate on Hoof Durability in Feedlot Heifers. Journal of Animal Science, 70 (Suppl.1), 313.
• Siciliano-Jones JL, Socha MT, Tomlinson DJ, DeFrain JM (2008). Effect of Trace Mineral Source on Lactaton Performance, Claw Integrity, and Fertility of Dairy Cattle. Journal of Dairy Science, 91, 1985-1995.
• Sogstad AM, Fjeldaas T, Osteras O (2011). Association of claw disorders with claw horn colour in norwegian red cattle – a cross-sectional study of 2607 cows from 112 herds. Acta Veterinaria Scandinavica, 53-59.
• Spain JN, Hardin D, Stevens B, Torne J (1993). Effect of Organic Zinc Supplemantation on Milk Somatic Cell Count and Incidence of Mammary Glands Infections of Lactating Dairy Cows. Journal of Dairy Science, 76 (Suppl), 265 (Abstr.).
• Tomlinson DJ, Mülling CH, Fakler TM (2004). Formation of Keratins in the Bovine Claw: Roles of Hormones, Minerals, and Vitamins in Functional Claw Integrity. Journal of Dairy Science, 87, 797–809.
• Ugel AR (1975). Bovine Keratohyaline: Anotomical, Histochemical, Ultrastructural, Immunologic and Biochemical Studies. Journal of Incvestigative Dermatology, 65(1), 118-26.
• Uchiada K, Mardebvu P, Ballard CS, Sniffen CJ, Carter MP (2001). Effect of Feding a Combination of Zinc, Manganese and Copper Aminoacid Complexes and Cobalt Glucoheptonate on Performance of Early Lactation High Producing Dairy Cows. Animal Feed Science and Technology, 93, 193-203.
• Underwood EJ (1977). Trace Elements in Human and Animal Nutrition. Academic Pres, New York.
• Winkler B, Margerison JK, Brennan C (2001). The Use of Texture Analysis to Asses the Structural Strenght of Hoof Horn of Dairy Cows. Department of Agriculture Food and Land Use, Seale – Hayne Faculty, Plymouth University, Newton Abbot, Devon, TQ12 6NQ. U.K. http://www.bsas.org.uk/wp-content/themes/bsas/ proceedings/Pdf2001/171.pdf Erişim: 13 Mart 2015.
• Weaver AD (1987). Influence of Feeding on The Health of The Bovine Foot. The Second Symposium on “Bovine Digital Diseases” 25-28th September, Skora, SE.