Detection of Antimicrobial Resistance in Staphylococcus aureus Isolated from Saanen Goats with Subclinical Mastitis

Yıl 2014, Cilt: 3 Sayı: 1, 284 - 287, 01.01.2014

Şükrü Kirkan Göksel Erbaş Serap Savaşan Neşe Uçan Saadet Gürpinar Volkan Özavci

Öz

Background/Aim: The aim of the present study is to describe the distribution of viral antigen localization in nonnervous tissues was described in goats naturally infected with caprine arthritis encephalitis virus.Materials and Methods: The milk samples were collected from one Saanen goats flock in the Western Turkey. A total of 280 half-udder milk samples 140 Saanen goats were collected in a single sampling time in a flock during the early lactation period. The milk samples were incolated to 5 % sheep blood agar incubated at 37oC for 24-48 hours. After that, all isolates were identified as S. aureus, using biochemical tests and using API ID32 STAPH system bioM´erieux® . Results and Conclusion: In this study, bacterial isolation was made from 138 49.3% out of 280 milk samples taken from subclinical mastitic milk of Saanen goat. Bacterial isolates were identified as S. aureus 90 32.1% . All S. aureus isolates n=90 were subjected to antimicrobial susceptibility testing. S. aureus isolates were resistant to oxytetracycline 100%, ampicillin 91% and vancomycin 60%. S. aureus are more in number as causative agents in subclinical mastitis and novobiocin is the most effective antibiotic in Saanen subclinical mastitis.

Anahtar Kelimeler

Saanen Goat, Subclinical Mastitis, S. aureus, Identification, Antimicrobial resistance.

Subklinik Mastitisli Saanen Keçilerinden İzole Edilen Staphylococcus aureus İzolatlarının Antimikrobiyel Dirençlerinin Belirlenmesi

Öz

Özbilgi/Amaç: Mastitis, bir ya da birden çok mikroorganizma tarafından oluşturulan meme bezinin yangısı ile karakterize bir hastalıktır. Süt içinde bulunan mikroorganizmaların varlığı genellikle mikrobiyel kültür yöntemi ile ortaya konabilmektedir. Staphylococcus aureus, keçilerde en sık teşhis edilen ve meme içi infeksiyonlara yol açan mikroorganizmadır. Bu çalışmanın amacı, Saanen keçilerinden izole edilen S. aureus etkenlerine karşı antimikrobiyel ajanların dirençliliğini tespit etmektir.Materyal ve Metot: Süt örnekleri, Batı Türkiye bölgesinde bulunan bir Saanen keçisi çiftliğinden toplanmıştır. Erken laktasyon periyodunda bulunan her bir hayvanın iki meme lobundan olmak üzere 280 adet 140 Saanen keçisi süt numunesi tek sefer örnekleme ile toplanmıştır. Toplanan süt numuneleri, % 5 koyun kanlı agara ekilmiş ve 37oC’de 24-48 saat inkube edilmiştir. Daha sonra, bütün izolatlar için biyokimyasal testler ve API ID32 STAPH sistemi bioM´erieux® kullanılmış ve izolatlar S. aureus olarak identifiye edilmiştir.Bulgular ve Sonuç: Bu çalışmada subklinik mastitisli Saanen keçilerinden alınan 280 adet süt numunesinden 138 % 49.3 adet bakteriyel izolasyon yapılmıştır. Bakteriyel izolatlardan 90 % 32.1 adedi S .aureus olarak identifiye edilmiştir. Bütün S. aureus n=90 suşları antimikrobiyel duyarlılık testine tabi tutulmuştur. S. aureus izolatları, oksitetrasikline karşı % 100, ampisiline karşı % 91 ve vankomisine karşı % 60 oranında dirençlilik göstermiştir. S. aureus etkeni, subklinik mastitiste etiyolojik ajan olarak sayıca daha fazla bulunmuştur ve novobiosin, Saanen keçilerinde görülen subklinik mastitis hastalığında söz konusu etkene karşı en etkili antibiyotik olarak tespit edilmiştir.

Anahtar Kelimeler

Saanen Keçisi, Subklinik Mastitis, S. aureus, İdentifikasyon, Antimikrobiyel dirençlilik

Kaynakça

• Bochev I and Russenova N (2005). Resistance of Staphylococcus spp strains Isolated from Goat with Subclinical Mastitis. Bulg J Vet Med, 8, 109-118.
• Clinical and Laboratory Standards Institute (2006a). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard. Clinical and Laboratory Standards
• Institute, Wayne, PA, USA, 7th edition, Document M7-A7.
• Clinical and Laboratory Standards Institute (2006b). Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Clinical and Laboratory Standards Institute,Wayne, PA, USA, Document M100-S16.
• Contreras A, Corrales JC, Sierra D and Marco J (1995). Prevalence and Aetiology of Non-Clinical Intramammary Infection in Murciano-Granadina Goats. Small Rumin Res, 17, 71- 78.
• Contreras A, Luengo C, Sanchez A and Corrales JC (2003). The Role of Intramammary Pathogens in Dairy Goats. Livest Product Sci, 79, 273-283.
• Contreras A, Sierra D, Sanchez A, Corrales JC, Marco JC, Paape MJ and Gonzalo C (2007). Mastitis in Small Ruminants. Small Rumin Res, 68, 145-153.
• Da Silva ER, Siqueira AP, Martins JCD, Ferreira WPB and Da Silva N (2004). Identification and In Vitro Antimicrobial Susceptibility of Staphylococcus Species Isolated From Goat Mastitis in The Northeast of Brazil. Small Ruminant Res, 55, 45-49.
• De Santis EPL, Mencarelli A, Nieddu MP, Farina S, Mazzette R, Sanna SR and Virdis S (2001). Efficacia Della Somministrazione Endomammaria di Cloxacillina Benzatina Per Il Trattamento Delle Infezioni Intramammarie Dell’Ovino Nel Corso Dell’asciutta. Large Anim Rev, 7, 39-47.
• Deinhofer M and Pernthaner A (1995). Staphylococcus spp. as Mastitis-related Pathogens in Goat Milk. Vet Microbiol, 43, 161-166.
• Donovan GA, Risco CA and Shearer JK (1992). Assessment of The Mammary System. Vet Clin North Am Food Anim Pract, 8, 361-372.
• East NE, Birnie EF and Farver TB (1987). Risk Factors Associated with Mastitis in Dairy Goats. Am J Vet Res, 48, 776-779.
• Holt JG, Kreig NR, Sneath PHA, Staley JT and Williams ST (1994). Bergey’s Manual of Determinative Bacteriology. Ed. By WR. Hensley, 194-196, 281-284, Williams and Wilkins, Baltimore, USA.
• Koneman EW, Allen SD, Janda WM, Schreckenberger PC and Winn WC (1997). Color Atlas and Textbook of Diagnostic Microbiology. Lippincott, New York, Fifth Edition, pp: 104.
• Manser PA (1986). Prevalence, Causes, and Laboratory Diagnosis of Subclinical Mastitis in the Goat. Vet Rec, 118, 552-554.
• Moroni P, Pisoni G, Vimercati C, Rinaldi M, Castiglioni B, Cremonesi P and Boettcher P (2005). Characterization of Staphylococcus aureus Isolated from Chronically Infected Dairy Goats. J Dairy Sci, 88, 3500- 3509.
• National Committee for Clinical Laboratory Standards (2002a). Development of in Vitro Susceptibility Testing Criteria and Quality Control Parameters for Veterinary Antimicrobial Agents; Approved Guidelin., National Committee for Clinical Laboratory Standards, Wayne, PA, USA, 2nd edition, Document M37-A2.
• National Committee for Clinical Laboratory Standards (2002b). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard. National Committee for Clinical Laboratory Standards, Wayne, PA, USA, 2nd edition, Document M31- A2.
• Poutrel B and Lerondelle C (1983). Cell Content of Goat Milk: California Mastitis Test, Coulter Counter, and Fossomatic for Predicting Half Infection. J Dairy Sci, 66, 2575-2579.
• Poutrel B, De Cremoux R, Ducelliez M and Verneau D (1997). Control of Intramammary Infections in Goats: Impact on Somatic Cell Counts. J Anim Sci, 75, 566-570.
• Schalm OW, Carroll EJ and Jain NC (1971). Bovine Mastitis. Lea and Febiger: Philadelphia, USA.
• Sears PM, Fettinger M and Marsh-Salin J (1987). Isolation of L form Variants After Antibiotic Treatment in Staphylococcus aureus Bovine Mastitis. J Am Vet Med Assoc, 191, 681-684.
• Shearer JK and Harris BJr (2003). Mastitis and Dairy Goats. DS 85 Published by University of Florida .
• Smith MC and Roguinsky M (1977). Mastitis and Other Diseases of The Goat’s Udder. J Am Vet Med Assoc, 171, 1241-1248.
• Smith MC and Sherman DM (1994). Mammary Gland and Milk Production. In: Goat Medicine, Lea and Febiger, Philadelphia, PA, USA, pp: 474-479.
• Thornsberry C, Burton PJ, Yee YC, Watts JL and Yancey RJ Jr (1997). The Activity of A Combination of Penicillin and Novobiocin Against Bovine Mastitis Pathogens: Development of A Disk Diffusion Test. J Dairy Sci, 80, 413-421.
• Walther B, Friedrich AW, Brunnberg L, Wieler LH and Lübke-Becker A (2006). Methicillin-resistant Staphylococcus aureus (MRSA) in Veterinary Medicine: A “New Emerging Pathogen”?. Berl Munch Tierarztl, 119, 222-232.