Serosurvey of Leptospira interrogans Serovars hardjo and pomona in Cattle in Nineveh Province, Iraq

Yıl 2013, Cilt: 2 Sayı: 1, 156 - 158, 01.01.2013

Eva Aisser Botres Ajaj Maab. I. Al- Farwachi

Öz

The purpose of the present study was to determine the serodiagnosis of anti-Leptospira hardjo and anti-L. pomona antibodies in cattle in Nineveh province, Iraq. For this purpose 96 sera were collected from adult native breed cattle 2 years old or over during an 7 month period from September 2012 to March 2013. Serodiagnosis of Leptospira interrogans serovars hardjo and pomona was carried out using a commercial ELISA. From a total of 96 sera, 6 6.3% were positive for Leptospira hardjo and only one animal 1 % was seropositive to L. pomona. These data suggest that leptospirosis is present among cattle in the study area.

Anahtar Kelimeler

Leptospirosis , Cattle , ELISA , Serodiagnosis , Leptospira hardjo.

Irak’ ta Nineveh Bölgesindeki Sığırlarda Leptospira interrogans hardjo ve pomona Serotiplerinin Serosürveyansı

Öz

Bu çalışmada Irak’ta Nineveh bölgesindeki sığırlarda anti-Leptospira hardjo ve anti-L. pomona antikorlarının belirlenmesi amaçlandı. Bu amaçla 7 aylık bir süreçte, Eylül 2012 ile Mart 2013 arasında, 2 yaş ve üzeri 96 yerli sığır ırkında serum örnekleri toplandı. Leptospira interrogans hardjo ve pomona serotipleri ticari olarak elde edilen ELİZA test kitleri ile belirlendi. Doksanaltı serum örneğinin 6’sı % 6.32 Leptospira hardjo yönünden, yalnızca 1’i %1 L. pomona yönünden pozitifti. Bu bulgular leptospirozis’in bu bölgedeki sığırlarda mevcut olduğunu göstermektedir.

Anahtar Kelimeler

ELISA, Leptospirozis, Leptospira hardjo, Serolojik tanı, Sığır

Kaynakça

• Al-Badrawi TYG, Habasha FG , Sultan SH ( 2010). Serological study of Leptospirosis in cattle, sheep and goats in Baghdad Province . Al- Anbar Journal Veterinary Science , 3,78-82.
• Adler B, Moctezuma A (2010). Leptospira and leptospirosis. Veteri- nary Microbiology, 140, 287–296.
• Bahaman AR , IBRAHIM AL , ADAM H (1987). Serological prevalence of leptospiral infection in domestic animals in West Malaysia. Epi- demiology and Infection , 99, 379-392
• Bolin CA. ( 2003). Leptospirosis. In: Fowler ME, Miller RE. Zoo and wild animal medicine. St. Louis, Missouri: WB Saunders , 699–702.
• Cole JR , Sulzer CR , Pursell AR ( 1973). Improved microtechnique for the leptospiral microscopic agglutination test. Applied Microbiolo- gy, 25, 976–980.
• Ezeh AO , Addo PB , Adesiyun AA , Bello CSS , Makinde A ( 1989). Serological prevalence of bovine leptospirosis in Plateau State, Ni- geria. Revue D’élevage et de Médecine Vétérinaire Des Pays Tropi- caux , 42,505-508.
• Dassanayake DLB ,Wimalaratna H , Agampodi SB , Liyanapathrirana VC , Piyarathna TACL , Goonapienuwala BL ( 2009). Evaluation of surveillance case definition in the diagnosis of leptospirosis, using the Microscopic Agglutination Test: a validation study. BMC Infec- tious Diseases, 9:48.
• El Jalii IM ( 2008). Comparison between ELISA and the Microscopic Agglutination a test for the Diagnosis of Bovine Leptospirosis Re- vue D’élevage et de Médecine Vétérinaire Des Pays Tropicaux , 61 (2) , 73-75.
• Faine S , Adler B , Bolin C , Perolat P (2000). Leptospira and Leptospi- rosis, 2nd Ed., Med. Sci. , Melbourne, Australia.
• Kavanagh OV , Skibinska A , Mackie DP, Montgomery JM, Logan EF, Ellis WA ( 2002). In Bovine leptospirosis: Validation of an ELISA to detect antibodies to Leptospira borgpetersenii serovar hardjo. Re- search in Veterinary Science, 72:26-26
• Kocabiyik A L, Cetin C (2004 ). Bovine leptospirosis in south Marmara region of Turkey : A serological survey. Revue de Médecine Vétéri- naire,155, 12, 606-608
• Larson RL , Maas J , Richey E , Bolin, C ( 2007). Herd Prevalence and Risk Factors of Leptospira Infection in Beef Cow/calf Operations in the United States: Leptospira borgpetwerseni Serovar Hardjo. Bo- vine Practitioner,41, 15-23.
• Leonard N , Mee JF , Snijders S, Mackie D (2004). Prevalence of an- tibodies to Leptospira interrogans serovar hardjo in bulk tank milk from unvaccinated Irish dairy herds. Irish Veterinary Journal , 57, 226-231.
• Miller DA , Wilson MA , Beran GW (1991). Survey to estimate prev- alence of Leptospira interrogans infection in mature cattle in the United States. American Journal of Veterinary Research , 52,1761– 1765.
• Machang URS, Mgdde G, Mpanduji D (1997 ). Leptospirosis in animals and humans in selected areas of Tanzania. Belgian Journal of Zool- ogy , 127, 97-104.
• Mariyar C P , Kumar AA, Thangapandian E , Amuthar R, Srivastavava SK ( 2006). Evaluation of a recombinant LipL41 antigen of Leptospi- ra interrogans serovar canicola in ELISA for
• serodiagnosis of bovine leptospirosis. Comparative Immunology, Mi- crobiology & Infectious Diseases., 29,269-277.
• Morgan LH , Mark AW , Tammy MA , Corrie LT , David AM (2007). Comparative serological study of Leptospira serovar hardjo geno- types for use in the microscopic agglutination test Journal of Veter- inary Diagnostic Investigation,19, 84–87 .
• Peregrine AS, Martin SW, Hopwood DA (2006). Neospora caninum and Leptospira serovar serostatus in dairy cattle in Ontario. Canadi- an Veterinary Journal ,47, 467–470.
• Pritchard DG (1986 ). National situation of leptospirosis in the United Kingdom. In: W.A. ELLIS and T.W.A. LITTLE (ed.) Present state of lep- tospirosis diagnosis and control. Martinus Nijhoff, Dordrecht, The Netherlands ,pp 221-233
• Radostits OM, Gay CC, Hinchcliff KW, Constable PD. (2007). In: Veter- inary Medicine: A Textbook of the Diseases of Cattle, Horses,sheep, Pigs and Goats. Saunders (Elsevier), Edinburgh, pp. 1094-1110.
• Rochat T (1998) .A review of leptospirosis in farm animals in Portugal. Revue scientifique et technique , 17, 699-712.
• Ryan EG , Leonard N, Luke OG, Michael LD , Simon JM (2012). Herd-level risk factors associated with Leptospira Hardjo seroprev- alence in Beef/Suckler herds inthe Republic of Ireland. Irish Veter- inary Journal ,65, 6
• Surujballi OM , Mallory M (2004 ) .An indirect enzyme linked immu- nosorbent assay for the detection of bovine antibodies to multiple Leptospira serovars .The Canadian Journal of Veterinary Research ,68,1–6.
• Thiermann AB (1984). Leptospirosis: current developments and trends. Journal of the American Veterinary Medical Association , 184, 722-725.
• Victoriano AF, Smythe LD , Gloriani-Barzaga N, Cavinta LL , Kasai T , Limpakarnjanarat K , Ong BL, Gongal G, Hall J , Coulombe CA , Yanagihara Y, Yoshida S, Adler B (2009). Leptospirosis in the Asia Pacific region. BMC Infectious Diseases , 9,147.
• Wagenaar J , Zuerner RL, Alt D, Bolin CA. ( 2000). Comparison of polymerase chain reaction assays with bacteriologic culture, immu- nofluorescence, and nucleic acid hybridization for detection of Lep- tospira borgpetersenii serovar hardjo in urine of cattle. American Journal of Veterinary Research , 61, 316–320.
• World Health Organization. ( 2003). Human Leptospirosis: Guidance for Diagnosis Surveillance and Control. Geneva: World Health Organi- zation,:109, ISBN 92-4-154589-5.