Biotyping of Staphylococcus Strains Isolated from Various Sources and Investigations on the Methicillin Resistance

Yıl 2012, Cilt: 1 Sayı: 1, 11 - 19, 01.01.2012

Selda Uysal Şükrü Kırkan

Öz

In this study, 74 Staphylococcus strains were isolated from 120 milk samples obtained from dairy cows with clinical and subclinical mastitis, 42 Staphylococcus strains were isolated from 60 wounds and 7 Staphylococcus strains were isolated from 20 urine samples. Biyotyping was carried out for all Staphylococcus strains. Slime values +, ++, +++, and - of coagulase positive Staphylococcus strains were determined as 15%, 12%, 32.8%, and 40.2%, respectively and coagulase negative strains were determined as 10.7%, 30.4%, 16.0%, and 42.9%, respectively. 41 33.3% Staphylococcus strains were found to be resistant to methicillin. S. aureus strains 46% , S. intermedius strains 37.5% , S. haemolyticus strains 43% and all S. cohnii subsp cohnii strains 100% presented similar resistance to vankomisin and teikoplanin. Of 48% to all of strains were resistant to penicilin and 85% of all strains were found to be sensitive to eritromisin, 83% of all strains were found to be sensitive to sulbactam + ampicillin, and 79% of all strains were found to be sensitive to sulphamethaxazole + trimethoprim.

Anahtar Kelimeler

Staphylococcus sp., Identification, Methisillin resistance, Antibiotic susceptibility test.

Değişik Kaynaklardan İzole Edilen Stafilokok Suşlarının Biyotiplendirilmesi ve Metisilin Direncinin Araştırılması

Öz

Bu araştırmada rutin teşhis laboratuarına getirilen klinik ve subklinik mastitisli ineklere ait 120 adet süt örneğinden 74 adet, 60 adet yara yeri svabı örneğinden 42 adet ve 20 adet idrar örneğinden ise 7 adet stafilokok suşu izole edildi. İzole edilen suşların identifikasyonları gerçekleştirilerek Stafilokok suşlarının tiplendirilmeleri yapıldı. Araştırmada koagulaz pozitif Stafilokok suşlarının slime değerleri +, ++, +++, - sırasıyla % 15, % 12, % 32.8, % 40.2; koagulaz negatif Stafilokok suşlarının ise slime değerleri +, ++, +++, - sırasıyla % 10.7, % 30.4, % 16.0, % 42.9 olarak belirlendi. Çalışmada tiplendirilmeleri gerçekleştirilen Stafilokok suşlarının 41 % 33.3 ’i metisiline dirençli bulundu. S. aureus suşları % 46 , S. intermedius suşları % 37.5 , S. haemolyticus suşları % 43 ve S. cohnii subsp. cohnii suşlarının tamamı %100 vankomisin ve teikoplanin’e aynı oranlarda dirençlilik göstermişlerdir. Tüm izolatların % 48’inin penisilin’e dirençli ve % 85 oranında eritromisine, % 83 oranında sulbactam + ampisilin’e ve % 79 oranında sulphamethaxsazole + trimethoprim’e duyarlı olduğu saptanmıştır.

Anahtar Kelimeler

Staphylococcus sp., İdentifikasyon, Methisilin Direnci, Antibiyotik Duyarlılık Testi

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