Olanzapin ile Timokinon'un Bağırsak Üzerindeki Etkilerinin Mikrobiyolojik Olarak Araştırılması

Öz

Çalışmamızın amacı, bir antipsikotik ilaç olanzapin (OL) ile indüklenen obez sıçanlarda timokinonun (TQ) etkisini incelemektir. Otuz beş dişi Spraque-Dawley sıçanı beş gruba ayrıldı (n = 7): Kontrol, OL (günlük 2 mg / kg OL), OL + TQ1 (2 mg / kg OL + 20 mg / kg TQ), OL + TQ2 (2 mg/kg OL + 40 mg/kg TQ) ve OL + TQ3 grubu (2 mg/kg OL + 80 mg/kg TQ). Tedavinin 15. gününde, analiz için bağırsak dokusu çıkarıldı. TQ tedavisinin, OL+TQ1, OL+TQ2 ve OL+TQ3 gruplarında bağırsak florasındaki Firmicutes ve Bacteroides düzeylerini değişen oranlarda etkilediği ve ayrıca TQ'nun apoptotik etkisinde önemli rolü olduğu tespit edilmiştir. Sonuç olarak, bu çalışma ile TQ tedavisinin OL'nin yan etkilerine karşı koruyucu özelliği olduğu belirlendi. TQ, terapötik etkinliği artırmak için etkili bir tedavi yöntemi olabilir.

Anahtar Kelimeler

• Olanzapin; Timokinon; Obezite; Firmicutes; Bacteroides.

Proje Numarası

SHMYOMAP/2015-0002

Microbiological Investigation of the Effects of Olanzapine with Thymoquinone on the Intestine

Öz

The aim of our study is to examine the effect of thymoquinone (TQ) in obese rats induced with the antipsychotic drug olanzapine (OL). Thirty-five female Spraque-Dawley rats were divided into five groups (n = 7): Control, OL (2 mg / kg OL daily), OL + TQ1 (2 mg / kg OL + 20 mg / kg TQ), OL + TQ2 (2 mg / kg OL + 40 mg / kg TQ) and the OL + TQ3 group (2 mg / kg OL + 80 mg / kg TQ). On the 15th day of treatment, intestinal tissue was removed for analysis. It has been found that TQ treatment affects the levels of Firmicutes and Bacteroides at varying rates in the intestinal flora in OL + TQ1, OL + TQ2, and OL + TQ3 groups, and also has a significant role in the apoptotic effect of TQ. In conclusion, with this study, it was determined that the treatment of TQ has a protective property against the side effects of OL. TQ can be an effective treatment method to increase therapeutic effectiveness.

Anahtar Kelimeler

• Olanzapine
• Thymoquinone
• Obesity
• Firmicutes
• Bacteroides

Destekleyen Kurum

Adıyaman Üniversitesi Bilimsel Araştırma Proje Birimi

Proje Numarası

SHMYOMAP/2015-0002

Teşekkür

I would like to thank Assoc. Prof.Dr. Hesna YİĞİT, who helped me in microbiological calculations in this study.

Kaynakça

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