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Characterization of amylase from Bacillus licheniformis and application in desizing process

Yıl 2025, Cilt: 15 Sayı: 2, 1 - 11, 31.12.2025
https://doi.org/10.37094/adyujsci.1532994
https://izlik.org/JA37XR89SL

Öz

There are more attention on the amylase enzymes in recent biotechnology because of application of them. To provide these needed enzymes, bacterial amylase enzymes are especially focused on. The bacterial strain was identified by sequencing of 16s ribosomal RNA coding gene BM Company at Ankara/Türkiye. After identified amylase production in starchy medium, the enzyme isolated partially, and optimum Ph and temperature parameters were characterized. The enzyme was applied to remove starch from starchy fabric. The last product of the enzyme was determined with TLC analysis. Its optimum pH and temperature were 5.0 and 45ºC, respectively. The potassium/iodine color change showed that enzyme was able to remove starch from fabric due to at least 8 of TEGEWA. Maltose is the last product. The overall results suggest that the extracted crude amylase can be a potential enzyme candidate for textile industries after further characterization in future.

Etik Beyan

yok

Destekleyen Kurum

yok

Proje Numarası

yok

Kaynakça

  • [1] Arabacı, N., Arıkan, B., Isolation and characterization of a cold-active, alkaline, detergent stable α-amylase from a novel bacterium Bacillus subtilis N8, Preparative Biochemistry and Biotechnology, 48(5), 419-426, 2018.
  • [2] Pandey, P., Nigam, P., Soccol, C.R., Soccol, V.T., Singh, D., Mohan, R., Advances in microbial amylases, Biotechnology and Applied Biochemistry, 31(2), 135-152, 2000.
  • [3] Sen, A., Kapila, R., Chaudhary, S., Nigam, A., Biotechnological Applications of Microbial Enzymes to Replace Chemicals in the Textile Industry-A Review, TEXTILE Association, 82(2), 68-73, 2021.
  • [4] Kocabay, S., Cetinkaya, S., Akkaya, B., Yenidünya, A.F., Characterization of thermostable β-amylase isozymes from Lactobacillus fermentum, International Journal of Biological Macromolecules, 93, 195-202, 2016.
  • [5] Akkaya, B., Yenidunya, A.F., Akkaya, R., Production and immobilization of a novel thermoalkalophilic extracellular amylase from bacilli isolate, International Journal of Biological Macromolecules, 50(4), 991-995, 2012.
  • [6] Ball, S., Guan, H.P., James, M., Myers, A., Keeling, P., Mouille, G., et.al., From glycogen to amylopectin: a model for the biogenesis of the plant starch granule, Cell, 86(3), 349-352, 1996.
  • [7] Mouille, G., Maddelein, M.L., Libessart, N., Talaga, P., Decq, A., Delrue, B., et. al., Preamylopectin processing: a mandatory step for starch biosynthesis in plants, The Plant Cell, 8(8), 1353-1366, 1996.
  • [8] Van Der Maarel, M.J., Van der Veen, B., Uitdehaag, J.C., Leemhuis, H., Dijkhuizen, L., Properties and applications of starch-converting enzymes of the α-amylase family, Journal of biotechnology, 94(2), 137-155, 2002.
  • [9] Hendriksen, H., Pedersen, S., Bisgard-Frantzen, H., A process for textile warp sizing using enzymatically modified starches, Patent Application, 1999.
  • [10] Yahya, S., Sohail, M., Khan, S.A., Characterization, thermal stabilization and desizing potential of amylase from A. tubingensis SY 1, The Journal of the Textile Institute, 113(6), 993-1000, 2022.
  • [11] Ghosh, K., Ray, M., Adak, A., Halder, S.K., Das, A., Jana, A., et. al., Role of probiotic Lactobacillus fermentum KKL1 in the preparation of a rice based fermented beverage, Bioresource Technology, 188, 161-168, 2015.
  • [12] Kubrak, O.I., Storey, J.M., Storey, K.B., Lushchak, V.I., Production and properties of α-amylase from Bacillus sp. BKL20, Canadian Journal of Microbiology, 56(4), 279-288, 2010.
  • [13] Elumalai, P., Lim, J.M., Park, Y.J., Cho, M., Shea, P.J., Oh, B.T., Enhanced amylase production by a Bacillus subtilis strain under blue light-emitting diodes, Preparative Biochemistry and Biotechnology, 49(2), 143-150, 2019.
  • [14] Salem,K., Elgharbi, F., Ben Hlima, H., Perduca, M., Sayari, A., Hmida‐Sayari, A., Biochemical characterization and structural insights into the high substrate affinity of a dimeric and Ca2+ independent Bacillus subtilis α‐amylase, Biotechnology Progress 36(4), e2964, 2020.
  • [15] Olufunke, F.O.T., Azeez, I.I., Purification and characterization of beta-amylase of Bacillus subtilis isolated from Kolanut Weevil, Journal of Biology and Life Science, 4(1), 68-78, 2012.
  • [16] Chinnammal, S.K., ArunKumar, K.V., Production and application of amylase enzyme for biodesizing, Journal of Environmental Nanotechnology, 2(2), 6-12, 2013.
  • [17] Bhatt, K., Lal, S., Srinivasan, R., Joshi, B., Bioconversion of agriculture wastes to produce α-amylase from Bacillus velezensis KB 2216: Purification and characterization, Biocatalysis and Agricultural Biotechnology, 28, 101703, 2020.
  • [18] Kiran, S., Kumari, S., Singh, A., Prabha, C., Kumari, S., Extracellular amylase production under submerged fermentation by Bacillus subtilis RK6, International Journal of Pharmacy and Biological Sciences, 8(1), 376-383, 2018.
  • [19] Ibrahim, D., Zhu, H.L., Yusof, N., Hong, L.S., Bacillus licheniformis BT5. 9 isolated from Changar Hot spring, Malang, Indonesia, as a potential producer of thermostable α-amylase, Tropical Life Sciences Research, 24(1), 71, 2013.
  • [20] Naganthran, A., Masomian, M., Rahman, R.N.Z.R.A., Ali, M.S.M., Nooh, H.M., Improving the efficiency of new automatic dishwashing detergent formulation by addition of thermostable lipase, protease and amylase, Molecules, 22(9), 1577, 2017.
  • [21] Bhatt, B.M., Trivedi, U.B., Patel, K.C., Extremophilic amylases: Microbial production and applications, Microbial Enzymes: Roles and Applications in Industries, Singapore: Springer Singapore, 185-205, 2020.
  • [22] Far, B.E., Ahmadi, Y., Khosroshahi, A.Y., Dilmaghani, A., Microbial alpha-amylase production: progress, challenges and perspectives, Advanced Pharmaceutical Bulletin, 10(3), 350, 2020.

Yıl 2025, Cilt: 15 Sayı: 2, 1 - 11, 31.12.2025
https://doi.org/10.37094/adyujsci.1532994
https://izlik.org/JA37XR89SL

Öz

Proje Numarası

yok

Kaynakça

  • [1] Arabacı, N., Arıkan, B., Isolation and characterization of a cold-active, alkaline, detergent stable α-amylase from a novel bacterium Bacillus subtilis N8, Preparative Biochemistry and Biotechnology, 48(5), 419-426, 2018.
  • [2] Pandey, P., Nigam, P., Soccol, C.R., Soccol, V.T., Singh, D., Mohan, R., Advances in microbial amylases, Biotechnology and Applied Biochemistry, 31(2), 135-152, 2000.
  • [3] Sen, A., Kapila, R., Chaudhary, S., Nigam, A., Biotechnological Applications of Microbial Enzymes to Replace Chemicals in the Textile Industry-A Review, TEXTILE Association, 82(2), 68-73, 2021.
  • [4] Kocabay, S., Cetinkaya, S., Akkaya, B., Yenidünya, A.F., Characterization of thermostable β-amylase isozymes from Lactobacillus fermentum, International Journal of Biological Macromolecules, 93, 195-202, 2016.
  • [5] Akkaya, B., Yenidunya, A.F., Akkaya, R., Production and immobilization of a novel thermoalkalophilic extracellular amylase from bacilli isolate, International Journal of Biological Macromolecules, 50(4), 991-995, 2012.
  • [6] Ball, S., Guan, H.P., James, M., Myers, A., Keeling, P., Mouille, G., et.al., From glycogen to amylopectin: a model for the biogenesis of the plant starch granule, Cell, 86(3), 349-352, 1996.
  • [7] Mouille, G., Maddelein, M.L., Libessart, N., Talaga, P., Decq, A., Delrue, B., et. al., Preamylopectin processing: a mandatory step for starch biosynthesis in plants, The Plant Cell, 8(8), 1353-1366, 1996.
  • [8] Van Der Maarel, M.J., Van der Veen, B., Uitdehaag, J.C., Leemhuis, H., Dijkhuizen, L., Properties and applications of starch-converting enzymes of the α-amylase family, Journal of biotechnology, 94(2), 137-155, 2002.
  • [9] Hendriksen, H., Pedersen, S., Bisgard-Frantzen, H., A process for textile warp sizing using enzymatically modified starches, Patent Application, 1999.
  • [10] Yahya, S., Sohail, M., Khan, S.A., Characterization, thermal stabilization and desizing potential of amylase from A. tubingensis SY 1, The Journal of the Textile Institute, 113(6), 993-1000, 2022.
  • [11] Ghosh, K., Ray, M., Adak, A., Halder, S.K., Das, A., Jana, A., et. al., Role of probiotic Lactobacillus fermentum KKL1 in the preparation of a rice based fermented beverage, Bioresource Technology, 188, 161-168, 2015.
  • [12] Kubrak, O.I., Storey, J.M., Storey, K.B., Lushchak, V.I., Production and properties of α-amylase from Bacillus sp. BKL20, Canadian Journal of Microbiology, 56(4), 279-288, 2010.
  • [13] Elumalai, P., Lim, J.M., Park, Y.J., Cho, M., Shea, P.J., Oh, B.T., Enhanced amylase production by a Bacillus subtilis strain under blue light-emitting diodes, Preparative Biochemistry and Biotechnology, 49(2), 143-150, 2019.
  • [14] Salem,K., Elgharbi, F., Ben Hlima, H., Perduca, M., Sayari, A., Hmida‐Sayari, A., Biochemical characterization and structural insights into the high substrate affinity of a dimeric and Ca2+ independent Bacillus subtilis α‐amylase, Biotechnology Progress 36(4), e2964, 2020.
  • [15] Olufunke, F.O.T., Azeez, I.I., Purification and characterization of beta-amylase of Bacillus subtilis isolated from Kolanut Weevil, Journal of Biology and Life Science, 4(1), 68-78, 2012.
  • [16] Chinnammal, S.K., ArunKumar, K.V., Production and application of amylase enzyme for biodesizing, Journal of Environmental Nanotechnology, 2(2), 6-12, 2013.
  • [17] Bhatt, K., Lal, S., Srinivasan, R., Joshi, B., Bioconversion of agriculture wastes to produce α-amylase from Bacillus velezensis KB 2216: Purification and characterization, Biocatalysis and Agricultural Biotechnology, 28, 101703, 2020.
  • [18] Kiran, S., Kumari, S., Singh, A., Prabha, C., Kumari, S., Extracellular amylase production under submerged fermentation by Bacillus subtilis RK6, International Journal of Pharmacy and Biological Sciences, 8(1), 376-383, 2018.
  • [19] Ibrahim, D., Zhu, H.L., Yusof, N., Hong, L.S., Bacillus licheniformis BT5. 9 isolated from Changar Hot spring, Malang, Indonesia, as a potential producer of thermostable α-amylase, Tropical Life Sciences Research, 24(1), 71, 2013.
  • [20] Naganthran, A., Masomian, M., Rahman, R.N.Z.R.A., Ali, M.S.M., Nooh, H.M., Improving the efficiency of new automatic dishwashing detergent formulation by addition of thermostable lipase, protease and amylase, Molecules, 22(9), 1577, 2017.
  • [21] Bhatt, B.M., Trivedi, U.B., Patel, K.C., Extremophilic amylases: Microbial production and applications, Microbial Enzymes: Roles and Applications in Industries, Singapore: Springer Singapore, 185-205, 2020.
  • [22] Far, B.E., Ahmadi, Y., Khosroshahi, A.Y., Dilmaghani, A., Microbial alpha-amylase production: progress, challenges and perspectives, Advanced Pharmaceutical Bulletin, 10(3), 350, 2020.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Enzimler
Bölüm Araştırma Makalesi
Yazarlar

Samet Kocabay 0000-0002-0120-2910

Proje Numarası yok
Gönderilme Tarihi 13 Ağustos 2024
Kabul Tarihi 25 Haziran 2025
Yayımlanma Tarihi 31 Aralık 2025
DOI https://doi.org/10.37094/adyujsci.1532994
IZ https://izlik.org/JA37XR89SL
Yayımlandığı Sayı Yıl 2025 Cilt: 15 Sayı: 2

Kaynak Göster

APA Kocabay, S. (2025). Characterization of amylase from Bacillus licheniformis and application in desizing process. Adıyaman University Journal of Science, 15(2), 1-11. https://doi.org/10.37094/adyujsci.1532994
AMA 1.Kocabay S. Characterization of amylase from Bacillus licheniformis and application in desizing process. ADYU J SCI. 2025;15(2):1-11. doi:10.37094/adyujsci.1532994
Chicago Kocabay, Samet. 2025. “Characterization of amylase from Bacillus licheniformis and application in desizing process”. Adıyaman University Journal of Science 15 (2): 1-11. https://doi.org/10.37094/adyujsci.1532994.
EndNote Kocabay S (01 Aralık 2025) Characterization of amylase from Bacillus licheniformis and application in desizing process. Adıyaman University Journal of Science 15 2 1–11.
IEEE [1]S. Kocabay, “Characterization of amylase from Bacillus licheniformis and application in desizing process”, ADYU J SCI, c. 15, sy 2, ss. 1–11, Ara. 2025, doi: 10.37094/adyujsci.1532994.
ISNAD Kocabay, Samet. “Characterization of amylase from Bacillus licheniformis and application in desizing process”. Adıyaman University Journal of Science 15/2 (01 Aralık 2025): 1-11. https://doi.org/10.37094/adyujsci.1532994.
JAMA 1.Kocabay S. Characterization of amylase from Bacillus licheniformis and application in desizing process. ADYU J SCI. 2025;15:1–11.
MLA Kocabay, Samet. “Characterization of amylase from Bacillus licheniformis and application in desizing process”. Adıyaman University Journal of Science, c. 15, sy 2, Aralık 2025, ss. 1-11, doi:10.37094/adyujsci.1532994.
Vancouver 1.Kocabay S. Characterization of amylase from Bacillus licheniformis and application in desizing process. ADYU J SCI [Internet]. 01 Aralık 2025;15(2):1-11. Erişim adresi: https://izlik.org/JA37XR89SL