Prokaryotik çeşitliliğin potansiyel biyoremediasyon enzimlerinin aşırı habitatlarda mevsimsel değişimi

Yıl 2025, Cilt: 15 Sayı: 2, 26 - 37, 31.12.2025

Suzan Sahin Dogan , Aytaç Kocabaş

https://doi.org/10.37094/adyujsci.1595458

Cited By: 1

https://izlik.org/JA45GR36SP

Öz

Biyoremediasyon uygulamalarında genellikle ekstremofil olmayan mikroorganizmalar kullanılmaktadır. Ancak bu uygulamalarda, ekstremofil olmayan mikroorganizmaların yüksek tuz konsantrasyonlarında organik kirleticileri giderimi, olumsuz tuz etkisinin üstesinden gelmek için bir ön arıtma işlemi gerektirmektedir. Halofil mikroorganizmalar, hipersalin ortamlara uyum sağlamalarını sağlayan önemli enzimatik yapılara sahiptir. Halofiller, hidrokarbonları tek karbon ve enerji kaynağı olarak etkili bir şekilde kullanabilmeleri nedeniyle yüksek tuz konsantrasyonlarında organik kirletici gideriminin tedavisi için uygun biyoremediasyon ajanları olarak öne çıkmaktadır. Bu çalışmada, 16S rDNA amplikon dizileme verilerini kullanarak Tuz Gölü'ndeki prokaryotik çeşitliliğin biyoremediasyon enzimlerini tahmin etmek için PICRUSt2 aracı uygulandı. Fonksiyonel analiz sonucunda, Tuz Gölü metagenom verilerinde biyoremediasyon süreciyle ilgili çeşitli enzimler tespit edildi. Arsenit taşıyıcı ATPaz (EC:3.6.3.16), arsenat redüktaz EC:1.20.4.1), (S)-2-haloasit dehalojenaz (EC:3.8.1.2), nitrat redüktaz (EC:1.7.99.4) ve katekol 2,3-dioksijenaz (EC:1.13.11.2) enzimleri yüksek bollukta gözlendi. Ayrıca, (S)-2-haloasit dehalojenaz, selenit su dikinazı, cıva (II) redüktazı, arsenit taşıyıcı ATPaz ve 4-hidroksifenilpiruvat dioksijenaz enzimleriyle ilişkili diziler önemli mevsimsel farklılık gösterdi.

Anahtar Kelimeler

PICRUSt2 , ekstremofilik , fonksiyonel genler , biyoremediasyon enzimleri , mevsimsel değişim

Proje Numarası

117Z966

Seasonal variation of potential bioremediation enzymes of prokaryotic diversity in extreme habitat

https://izlik.org/JA45GR36SP

Öz

In bioremediation applications, non-extremophilic microorganisms are generally used. However, in these applications, the removal of organic pollutants by non-extremophilic microorganisms at high salt concentrations requires a pretreatment process to overcome the negative salt effect. Halophilic microorganisms have important enzymatic structures that allow them to adapt to hypersaline environments. Halophiles stand out as suitable bioremediation agents for the treatment of organic pollutant removal at high salt concentrations because they can effectively use hydrocarbons as sole carbon and energy sources. In this study PICRUSt2 tool was applied to predict bioremediation enzymes of prokaryotic diversity in Tuz Lake using 16S rDNA amplicon sequencing data. As a result of functional analysis, various enzymes related to bioremediation process were detected in Tuz Lake metagenome data. Arsenite transporter ATPase (EC:3.6.3.16), arsenate reductase EC:1.20.4.1), (S)-2-haloacid dehalogenase (EC:3.8.1.2), nitrate reductase (EC:1.7.99.4), and catechol 2,3-dioxygenase (EC:1.13.11.2) enzymes were observed in high abundance. Moreover, the sequences associated with (S)-2-haloacid dehalogenase, selenite water dikinase, mercury (II) reductase, arsenite transporter ATPase and 4-hydroxyphenylpyruvate dioxygenase enzymes were showed significant seasonal difference.

Anahtar Kelimeler

PICRUSt2 , extremophilic , functional genes , bioremediation enzymes , seasonal variation

Etik Beyan

We declare that this study is among the studies that do not require ethics committee approval.

Destekleyen Kurum

TUBITAK

Proje Numarası

117Z966

Teşekkür

This study is supported by TUBITAK (Turkish Scientific and Technical Research Council) with project number 117Z966.

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