Pistacia terebinthus Galinin Antimikrobiyal, Antioksidan ve Enzim İnhibitör Aktivitesinin Araştırılması

Öz

Bu çalışma, oluşumu Slavum Aff. Mordvilkoi tarafından indüklenen Pistacia terebinthus galinin antioksidan, antimikrobiyal ve enzim inhibisyonu aktivitelerini değerlendirmektedir. Gal ekstreleri petrol eteri, aseton, etanol ve su kullanılarak elde edilmiştir. Antioksidan aktivite, DPPH, ABTS ve CUPRAC yöntemleri ile belirlenmiş, toplam fenolik ve flavonoid içerikleri ölçülmüştür. Antimikrobiyal etkinlik, Gram-pozitif (Staphylococcus aureus, Streptococcus pyogenes), Gram-negatif (Escherichia coli, Pseudomonas aeruginosa) bakterilere ve mayaya (Candida albicans) karşı test edilmiştir. Enzim inhibisyonu, asetilkolinesteraz (AChE), bütirilkolinesteraz (BChE), tirozinaz ve üreaz enzimleri üzerinde değerlendirilmiştir.Sonuçlar, aseton ve etanol ekstrelerinin en yüksek antioksidan aktiviteye sahip olduğunu, ancak su ekstraktının da belirli testlerde yüksek aktivite gösterdiğini ortaya koymuştur. Antimikrobiyal testlerde, Gram-pozitif bakteriler ve C. albicans en duyarlı mikroorganizmalar olurken, E. coli dirençli bulunmuştur. Enzim inhibisyonu açısından, aseton ve etanol ekstreleri AChE, BChE ve tirozinaz üzerinde güçlü etki gösterirken, su ekstraktı üreaz enzim inhibisyonunda en yüksek aktiviteye sahip olmuştur. Bu bulgular, P. terebinthus gal ekstrelerinin doğal antioksidan, antimikrobiyal ve enzim inhibitörü olarak değerlendirilebileceğini göstermektedir. Gelecekte yapılacak çalışmalar, biyoaktif bileşiklerin izolasyonu ve farmasötik, gıda koruma ve kozmetik alanlarında potansiyel uygulamalarına odaklanmalıdır.

Anahtar Kelimeler

• Pistacia terebinthus
• Gal
• Antimikrobiyal Aktivite
• Antioksidan Aktivite
• Enzim inhibisyonu

Investigation of Antimicrobial, Antioxidant and Enzyme Inhibitory Activity of Pistacia terebinthus Gall

Öz

This study evaluates the antioxidant, antimicrobial, and enzyme inhibitory activities of Pistacia terebinthus L. gall induced by Slavum Aff. Mordvilkoi. The galls were extracted using petroleum ether, acetone, ethanol, and water. Antioxidant activity was assessed via DPPH, ABTS, and CUPRAC assays, along with total phenolic and flavonoid content determination. Antimicrobial activity was tested against Gram-positive (Staphylococcus aureus, Streptococcus pyogenes) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria, as well as yeast (Candida albicans). Enzyme inhibition was evaluated for acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, and urease. The results indicate that acetone and ethanol extracts exhibited the highest antioxidant activity, while the water extract also demonstrated significant activity in specific assays. Antimicrobial testing revealed that Gram-positive bacteria and C. albicans were the most susceptible, whereas E. coli exhibited resistance. For enzyme inhibition, acetone and ethanol extracts showed strong inhibitory effects on AChE, BChE, and tyrosinase, while the water extract exhibited the highest urease inhibition. These findings suggest that P. terebinthus gall extracts could serve as natural antioxidants, antimicrobials, and enzyme inhibitors. Future studies should focus on isolating bioactive compounds and exploring their potential applications in pharmaceuticals, food preservation, and cosmetics.

Anahtar Kelimeler

• Pistacia terebinthus
• Gall
• Antimicrobial Activity
• Antioxidant Activity
• Enzyme Inhibion

Kaynakça

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