Bir Beyaz Çürükçül Fungus ve Çeşitli Bakteriyel Suşlar ile Tekstil Boyasının Renginin Gideriminin Karşılaştırmalı Olarak Araştırılması

Öz

Bu çalışmanın amacı, üç farklı bakteriyel suş Bacillus megaterium A1 (A1), Gordonia sp. MC-D1 (D1) and Bacillus pumilus D3 (D3) ve ayrıca bir beyaz çürükçül fungusun (Pleurotus ostreatus) kullanımı ile Reaktif Mavi 171’ in (RM 171) renginin gideriminin karşılaştırmalı olarak araştırılmasıdır. Test edilen tüm bakteriler 150 mg/L konsantrasyonda RM 171 boyası ile 24-72 saat boyunca statik ve çalkalamalı (150 rpm) koşullarda inkübe edilirken fungus 150 mg/L boya ile aynı koşullarda 3-24 saat boyunca inkübe edildi. En yüksek bakteriyel renk giderimleri statik koşullarda 72 saat inkübasyon sonrasında elde edildi ve maksimum renk giderim oranları A1, D1 ve D3 için sırasıyla 84, 83 ve %75 olarak saptandı. Bununla birlikte, Pleurotus ostreatus ile statik koşullarda, 24 saat inkübasyon sonrası RM 171 boyasının rengi %93 oranında giderildi. Benzer sonuçlar A1 hariç test edilen fungal ve bakteriyel suşların çalkalamalı çalışmalarından da elde edildi. A1, D1 ve D3 ile 150 rpm ve 72 saatte elde edilen maksimum renk giderim değerleri sırasıyla 30, 88 ve %89’ dur. Pleurotus ostreatus’ un en yüksek renk giderim aktiviteleri hem statik hem de çalkalamalı (150 rpm) koşullarda %93 idi. Ayrıca lakkaz varlığının tespiti amacıyla SBM ve 150 mg/L RB 171 içeren SBM’den elde edilen fungal kültür sıvılarının zimogram analizleri de yapıldı.

Anahtar Kelimeler

• Bakteri
• Renk giderimi
• Tekstil boyası
• Beyaz çürükçül fungus
• Zimogram

Comparatively Investigation of Textile Dye Decolorization by a White Rot Fungus and Various Bacterial Strains

Öz

The aim of this study is to comparatively investigate the decolorization of Reactive Blue 171 (RB 171) by using three different bacterial strains as Bacillus megaterium A1 (A1), Gordonia sp. MC-D1 (D1) and Bacillus pumilus D3 (D3) and also a white rot fungus (Pleurotus ostreatus). All tested bacteria were incubated with RB 171 dye at 150 mg/L concentration for 24-72 h under static or agitated (150 rpm) conditions while the fungus was incubated with 150 mg/L of the dye under the same conditions for 3-24 h. The highest bacterial decolorization values were obtained after 72 h of incubation under static conditions and the maximum decolorization rates were detected as 84, 83 and 75% for A1, D1 and D3, respectively. However, the color of RB 171 dye was removed at 93% rate by Pleurotus ostreatus under static conditions after 24 h of incubation. Similar results were also obtained from the agitated studies of the tested fungal and bacterial strains except A1. The maximum decolorization values obtained with A1, D1 and D3 at 150 rpm, 72 h were 30, 88 and 89%, respectively. The highest decolorization activities of Pleurotus ostreatus were 93% for both static and agitated (150 rpm) conditions. In addition, zymogram analyzes of the fungal culture fluids obtained from SBM and SBM containing 150 mg/L RB 171 were also performed to detect the presence of laccase.

Anahtar Kelimeler

• Bacterium
• Decolorization
• Textile dye
• White rot fungus
• Zymogram

Kaynakça

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