Araştırma Makalesi
BibTex RIS Kaynak Göster
Yıl 2020, Cilt: 10 Sayı: 1, 29 - 39, 25.06.2020
https://doi.org/10.37094/adyujsci.680580

Öz

Kaynakça

  • [1] Gangadharan, D., Sivaramakrishnan S., Nampoothiri, K.M., Sukumaran, R.K., Pandey, A., Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens, Bioresource Technology, 99, 4597–4602, 2008.
  • [2] Lévêque, E., Janeček, Š., Haye, B., Belarbi, A., Thermophilic archaeal amylolytic enzymes, Enzyme and Microbial Technology, 26, 3–14, 2000.
  • [3] Ashwini, K., Gaurav, K., Karthik, L., Bhaskara Rao, K.V., Optimization, production and partial purification of extracellular α-amylase from Bacillus sp. Marini, Archives of Applied Science Research, 3, 33–42, 2011.
  • [4] Vijayalakshmi, K., Sushma, S.A., Chander, P., Isolation and characterization of Bacillus subtilis KC3 for amylolytic activity, International Journal of Bioscience Biochemistry and Bioinformatics, 2, 33, 2012.
  • [5] Sanchez, A.C., Ravanal, M.C., Andrews, B.A., Asenjo, J.A., Heterologous expression and biochemical characterization of a novel cold active α-amylase from the Antarctic bacteria Pseudoalteromonas sp. 2-3, Protein Expression and Purification, 155, 78–85, 2019.
  • [6] Elmansy, E.A., Asker, M.S., El-Kady, E.M., Hassanein, S.M., El-Beih, F.M., Production and optimization of α-amylase from thermo-halophilic bacteria isolated from different local marine environments, Bulletin of the National Research Centre, 42, 31, 2018.
  • [7] Souza, P.M., Magalhaes, P.O., Applications of microbial α-amylase in industry-a review, Brazilian Journal of Microbiology, 41, 850–61, 2010.
  • [8] Pandey, A., Nigam, P., Soccol, C.R., Socco, V.T., Singh, D., Mohan, R., Advances in microbial amylases, Biotechnology and Applied Biochemistry, 31, 135–52, 2000.
  • [9] Lipson, D.A., Schadt, C.W., Schmidt, S.K., Changes in Soil Microbial Community Structure and Function in an Alpine Dry Meadow Following Spring Snow Melt, Microbial Ecology, 43, 307–314, 2002.
  • [10] Panneerselvam, T., Elavarasi, S., Isolation of α-Amylase Producing Bacillus subtilis from Soil, International Journal of Current Microbiology and Applied, 4: 543–552, 2015.
  • [11] Schallmey, M., Singh, A., Ward, O.P., Developments in the use of Bacillus species for industrial production, Canadian Journal of Microbiology, 50, 1, 2004.
  • [12] Božić, N., Ruiz, J., Santίn, J.L., Vujćić, Z., Production and properties of the highly efficient raw starch digesting α-amylase from a Bacillus licheniformis ATCC 9945a, Biochemical Engineering Journal, 53, 203–209, 2011.
  • [13] Pranay, K., Padmadeo, S.R., Jha, V., Prasad, B., Screening and identification of amylase producing strains of Bacillus, Journal of Applied Biology Biotechnology, 1–6, 2019.
  • [14] Bernfeld, P., Enzymes carbohydrate metabolism, ln Methods ln Enzymology, Academic Press, 17: 149–158, 1955.
  • [15] Lowry, O.H., Roserbrough, N.J., Farr, A.L., Randall, R., Protein measure ment with folin phenol reagent, Journal of Biological Chemistry, 193, 265–275, 1951.
  • [16] Enez, B., Işgının Kök Toprağından Bacillus atrophaeus’un İzolasyonu ve Tanımlanması: α-Amilaz’ın Elde Edilmesi ve Karakterizasyonu, European Journal of Science and Technology, 177, 36–743, 2019.
  • [17] Hamilton, L.M., Kelly, C.T., Fogarty, W.M., Production and properties of the raw starch digesting α-amylase of Bacillus sp. IMD 435, Process Biochemistry, 35, 27–31, 1999.
  • [18] Asgher, M., Asad, M.J., Rahman, S.U., Legge, R.L., A thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing, Journal of Food Engineering, 79, 950–955, 2007.
  • [19] Behal, A., Sharma, M.K., Puri, P., Singh, J., Batra, N., Characterization of alkaline α-amylase from Bacillus sp. AB 04, International Journal of Agriculture and Biology, 8, 80–83, 2006.
  • [20] Saxena, R.K., Dutt, K., Agarwal, L., Nayyar, P., A highly thermostable and alkaline amylase from a Bacillus sp. PN5, Bioresource Technology, 98, 260–265, 2007.
  • [21] Ozdemir, S., Aguloglu Fincan, S., Karakaya, A., Enez, B., A Novel Raw Starch Hydrolyzing Thermostable α-Amylase Produced by Newly Isolated Bacillus mojavensis SO-10: Purification, Characterization and Usage in Starch Industries, Brazilian Archives of Biology and Technology, 61:e18160399, 2018.
  • [22] Abd-Elaziz, A. M., Karam, E.A., Ghanem, M.M., Moharam, M.E., Kansoh, A.L., Production of a novel α-amylase by Bacillus atrophaeus NRC1 isolated from honey: Purification and characterization, International Journal of Biological Macromolecules, 148, 292–301, 2020.
  • [23] Vaseekaran, S., Balakumar, S., Arasaratnam, V., Isolation and identification of a bacterial strain producing thermostable α-amylase, Tropical Agricultural Research, 22, 1–11, 2010.
  • [24] Aguloglu Fincan, S., Enez, B., Production, purification, and characterization of thermostable α-amylase from thermophilic Geobacillus stearothermophilus, Starch/Stärke, 66, 182–189. 2014.
  • [25] Wang, Y., Hu, H., Ma, J., Yan, Q., Liu, H., Jiang, Z., A novel high maltose-forming α-amylase from Rhizomucor miehei and its application in the food industry, Food Chemistry, 305, 125447, 2020.

Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer Lab.: Obtaining and Characterization of α-Amylase

Yıl 2020, Cilt: 10 Sayı: 1, 29 - 39, 25.06.2020
https://doi.org/10.37094/adyujsci.680580

Öz

    α-Amylase (1,4-α D-glucanohydrolase; EC 3.2.1.1) is one of the enzymes that can be used in a number of industrial processes such as bakery, textile, paper, detergents, and is the most important industrial product that plays an important role in the production of bioprotein and the fermentation of starch. The present research aims to the information gap in this area by performing bacterial isolation from the root of Astragalus gummifer. Bacterial isolation was performed using serial dilution technique. 16S rRNA analysis was performed for species identification of the microorganism. Isolated microorganism was determined to be gram (+), moving as a result of biochemical tests. Biochemical tests; Catalase, hemolysis, glucose and lactose tests positive (+); indole, H2S and urease tests showed negative (-) results. Soil samples were analyzed by ICP-MS. As a result of the analysis, it was determined that Al, Cr, Cu, Co, Fe, Pb, Mn and Zn were found in excess amount in the isolated bacteria. The optimal reproductive function of the bacteria was determined at 72 hours, 30 oC and pH 7.0. The maximum production time of α-amylase from Bacillus sp. was 24 hours, 30 oC and pH 7.0. Bacteria identified and found to produce amylase were determined to be used in different biotechnological areas.

Kaynakça

  • [1] Gangadharan, D., Sivaramakrishnan S., Nampoothiri, K.M., Sukumaran, R.K., Pandey, A., Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens, Bioresource Technology, 99, 4597–4602, 2008.
  • [2] Lévêque, E., Janeček, Š., Haye, B., Belarbi, A., Thermophilic archaeal amylolytic enzymes, Enzyme and Microbial Technology, 26, 3–14, 2000.
  • [3] Ashwini, K., Gaurav, K., Karthik, L., Bhaskara Rao, K.V., Optimization, production and partial purification of extracellular α-amylase from Bacillus sp. Marini, Archives of Applied Science Research, 3, 33–42, 2011.
  • [4] Vijayalakshmi, K., Sushma, S.A., Chander, P., Isolation and characterization of Bacillus subtilis KC3 for amylolytic activity, International Journal of Bioscience Biochemistry and Bioinformatics, 2, 33, 2012.
  • [5] Sanchez, A.C., Ravanal, M.C., Andrews, B.A., Asenjo, J.A., Heterologous expression and biochemical characterization of a novel cold active α-amylase from the Antarctic bacteria Pseudoalteromonas sp. 2-3, Protein Expression and Purification, 155, 78–85, 2019.
  • [6] Elmansy, E.A., Asker, M.S., El-Kady, E.M., Hassanein, S.M., El-Beih, F.M., Production and optimization of α-amylase from thermo-halophilic bacteria isolated from different local marine environments, Bulletin of the National Research Centre, 42, 31, 2018.
  • [7] Souza, P.M., Magalhaes, P.O., Applications of microbial α-amylase in industry-a review, Brazilian Journal of Microbiology, 41, 850–61, 2010.
  • [8] Pandey, A., Nigam, P., Soccol, C.R., Socco, V.T., Singh, D., Mohan, R., Advances in microbial amylases, Biotechnology and Applied Biochemistry, 31, 135–52, 2000.
  • [9] Lipson, D.A., Schadt, C.W., Schmidt, S.K., Changes in Soil Microbial Community Structure and Function in an Alpine Dry Meadow Following Spring Snow Melt, Microbial Ecology, 43, 307–314, 2002.
  • [10] Panneerselvam, T., Elavarasi, S., Isolation of α-Amylase Producing Bacillus subtilis from Soil, International Journal of Current Microbiology and Applied, 4: 543–552, 2015.
  • [11] Schallmey, M., Singh, A., Ward, O.P., Developments in the use of Bacillus species for industrial production, Canadian Journal of Microbiology, 50, 1, 2004.
  • [12] Božić, N., Ruiz, J., Santίn, J.L., Vujćić, Z., Production and properties of the highly efficient raw starch digesting α-amylase from a Bacillus licheniformis ATCC 9945a, Biochemical Engineering Journal, 53, 203–209, 2011.
  • [13] Pranay, K., Padmadeo, S.R., Jha, V., Prasad, B., Screening and identification of amylase producing strains of Bacillus, Journal of Applied Biology Biotechnology, 1–6, 2019.
  • [14] Bernfeld, P., Enzymes carbohydrate metabolism, ln Methods ln Enzymology, Academic Press, 17: 149–158, 1955.
  • [15] Lowry, O.H., Roserbrough, N.J., Farr, A.L., Randall, R., Protein measure ment with folin phenol reagent, Journal of Biological Chemistry, 193, 265–275, 1951.
  • [16] Enez, B., Işgının Kök Toprağından Bacillus atrophaeus’un İzolasyonu ve Tanımlanması: α-Amilaz’ın Elde Edilmesi ve Karakterizasyonu, European Journal of Science and Technology, 177, 36–743, 2019.
  • [17] Hamilton, L.M., Kelly, C.T., Fogarty, W.M., Production and properties of the raw starch digesting α-amylase of Bacillus sp. IMD 435, Process Biochemistry, 35, 27–31, 1999.
  • [18] Asgher, M., Asad, M.J., Rahman, S.U., Legge, R.L., A thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing, Journal of Food Engineering, 79, 950–955, 2007.
  • [19] Behal, A., Sharma, M.K., Puri, P., Singh, J., Batra, N., Characterization of alkaline α-amylase from Bacillus sp. AB 04, International Journal of Agriculture and Biology, 8, 80–83, 2006.
  • [20] Saxena, R.K., Dutt, K., Agarwal, L., Nayyar, P., A highly thermostable and alkaline amylase from a Bacillus sp. PN5, Bioresource Technology, 98, 260–265, 2007.
  • [21] Ozdemir, S., Aguloglu Fincan, S., Karakaya, A., Enez, B., A Novel Raw Starch Hydrolyzing Thermostable α-Amylase Produced by Newly Isolated Bacillus mojavensis SO-10: Purification, Characterization and Usage in Starch Industries, Brazilian Archives of Biology and Technology, 61:e18160399, 2018.
  • [22] Abd-Elaziz, A. M., Karam, E.A., Ghanem, M.M., Moharam, M.E., Kansoh, A.L., Production of a novel α-amylase by Bacillus atrophaeus NRC1 isolated from honey: Purification and characterization, International Journal of Biological Macromolecules, 148, 292–301, 2020.
  • [23] Vaseekaran, S., Balakumar, S., Arasaratnam, V., Isolation and identification of a bacterial strain producing thermostable α-amylase, Tropical Agricultural Research, 22, 1–11, 2010.
  • [24] Aguloglu Fincan, S., Enez, B., Production, purification, and characterization of thermostable α-amylase from thermophilic Geobacillus stearothermophilus, Starch/Stärke, 66, 182–189. 2014.
  • [25] Wang, Y., Hu, H., Ma, J., Yan, Q., Liu, H., Jiang, Z., A novel high maltose-forming α-amylase from Rhizomucor miehei and its application in the food industry, Food Chemistry, 305, 125447, 2020.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Biyoloji
Yazarlar

Bariş Enez

Yayımlanma Tarihi 25 Haziran 2020
Gönderilme Tarihi 27 Ocak 2020
Kabul Tarihi 3 Mart 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 10 Sayı: 1

Kaynak Göster

APA Enez, B. (2020). Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer Lab.: Obtaining and Characterization of α-Amylase. Adıyaman University Journal of Science, 10(1), 29-39. https://doi.org/10.37094/adyujsci.680580
AMA Enez B. Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer Lab.: Obtaining and Characterization of α-Amylase. ADYU J SCI. Haziran 2020;10(1):29-39. doi:10.37094/adyujsci.680580
Chicago Enez, Bariş. “Isolation and Identification of Bacillus Sp. From Root Soil of the Astragalus Gummifer Lab.: Obtaining and Characterization of α-Amylase”. Adıyaman University Journal of Science 10, sy. 1 (Haziran 2020): 29-39. https://doi.org/10.37094/adyujsci.680580.
EndNote Enez B (01 Haziran 2020) Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer Lab.: Obtaining and Characterization of α-Amylase. Adıyaman University Journal of Science 10 1 29–39.
IEEE B. Enez, “Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer Lab.: Obtaining and Characterization of α-Amylase”, ADYU J SCI, c. 10, sy. 1, ss. 29–39, 2020, doi: 10.37094/adyujsci.680580.
ISNAD Enez, Bariş. “Isolation and Identification of Bacillus Sp. From Root Soil of the Astragalus Gummifer Lab.: Obtaining and Characterization of α-Amylase”. Adıyaman University Journal of Science 10/1 (Haziran 2020), 29-39. https://doi.org/10.37094/adyujsci.680580.
JAMA Enez B. Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer Lab.: Obtaining and Characterization of α-Amylase. ADYU J SCI. 2020;10:29–39.
MLA Enez, Bariş. “Isolation and Identification of Bacillus Sp. From Root Soil of the Astragalus Gummifer Lab.: Obtaining and Characterization of α-Amylase”. Adıyaman University Journal of Science, c. 10, sy. 1, 2020, ss. 29-39, doi:10.37094/adyujsci.680580.
Vancouver Enez B. Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer Lab.: Obtaining and Characterization of α-Amylase. ADYU J SCI. 2020;10(1):29-3.

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