Calculation of Dynamic Properties of Drug-Added Aqueous Solutions with T1 and T2 Relaxation Times

Yıl 2022, Cilt: 12 Sayı: 2, 299 - 314, 30.12.2022

Sibel Korunur

https://doi.org/10.37094/adyujsci.1200682

Öz

The development of modern spectroscopic methods has facilitated and accelerated structure analysis. The NMR method is the most popular way to perform structural analysis of compounds with very complex structures. D2O is a solvent that is frequently used in NMR analysis of both chemical molecules and many biological molecules such as drugs, proteins, and enzymes. In this paper, the study of residual water in proton drug-added protein solutions was carried out via NMR relaxation. The spin-lattice (T1) and the spin-spin relaxation (T2) times of residual water in drug-added protein solutions were studied depending on temperature by Avance Bruker 400 MHz 1H-NMR Spectrometer, and activation energies (E_a) and rotational correlation times (τ_0 and τ_c) have been determined for T1 and T2 relaxation times.

Anahtar Kelimeler

D2O, Proton NMR, Relaxation times, Activation energy, Flurouacil

İlaç Katkılı Sulu Çözeltilerin Dinamik Özelliklerinin T1 ve T2 Rölaksasyon Zamanları ile Hesaplanması

Öz

Modern spektroskopik yöntemlerin gelişimi, yapı analizini kolaylaştırmış ve hızlandırmıştır. NMR yöntemi, çok karmaşık yapılara sahip bileşiklerin yapısal analizini gerçekleştirmenin en popüler yoludur. D2O, hem kimyasal moleküllerin hem de ilaçlar, proteinler, enzimler gibi birçok biyolojik molekülün NMR analizinde sıklıkla kullanılan bir çözücüdür. Bu çalışmada, ilaç katkılı protein çözeltilerinde residual su çalışması, proton NMR rölaksasyonu yoluyla gerçekleştirilmiştir. Avance Bruker 400 MHz 1H-NMR Spektrometresi ile ilaç katkılı protein çözeltilerinde residual suyun spin-örgü (T1) ve spin-spin rölaksasyon (T2) süreleri sıcaklığa bağlı olarak ölçülmüş, T1 ve T2 rölaksasyon süreleri için aktivasyon enerjileri (E_a) ile τ_0 ve τ_c dönme korelasyon zamanları belirlenmiştir.

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