Assessment of the Developmental Profiles of ZHY3 and W303 Yeast Strains in Zinc-Modified Media

Yıl 2024, Cilt: 14 Sayı: 2, 50 - 58, 31.12.2024

Beyza Yuksel M. Aydin Akbudak

https://doi.org/10.37094/adyujsci.1565012

Öz

All organisms require zinc to sustain their vital functions. In the case of Zn deficiency, plant production, as well as animal and human health, are adversely affected. Baker's yeast (Saccharomyces cerevisiae) is a model organism for scientific research and is commonly used in studies of metal deficiency/toxicity. This study aims to identify the first stage in the identification of Zn uptake genes, which determines the conditions of Zn-deficient environments that inhibit yeast development. The YNB medium, which is the basic nutrient medium for yeast cultivation, contains enough Zn conducive to yeast growth. Chelating agents (EGTA or EDTA) have been added to the YNB medium to create conditions of insufficient Zn. The yeast strains ZHY3 (Zn uptake gene mutant) and W303 (wild type – WT) were tested in these environments. The growth profiles of the yeasts were compared in nutrient media containing different concentrations of the chelating agents. The concentrations of EGTA and EDTA that inhibited the growth of the W303 strain were determined to be 25 mM and 1.6 mM, respectively, while the concentrations that inhibited the growth of the ZHY3 strain were determined to be 12.5 mM and 0.01 mM, respectively. These findings are significant for understanding the effects of Zn deficiency on different yeast strains and highlighting the importance of Zn uptake genes. It is believed that the identified concentrations and the results obtained will contribute to studies related to Zn uptake and Zn uptake genes.

Anahtar Kelimeler

Yeast, Zinc, ZHY3, W303, EDTA, EGTA

Proje Numarası

FYL-2024-6575

Kaynakça

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