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Trikalsiyum Fosfat Çözebilen Bazı Bakteri ve Mikrofungusların İzolasyonu ve Karakterizasyonu

Yıl 2013, Cilt: 1 Sayı: 1, 11 - 20, 01.06.2013

Öz

Bu çalışmada; sıcaklık, pH, tuzluluk vb. özellikler bakımından farklılık gösteren habitatlardan çeşitli bitkilere ait kök örnekleri ve rizosfer toprakları toplandı. Toprak dilüsyonları ve kök özütlerinden, bakteri ve mikrofunguslar için önerilen genel besi yerlerine ekim yapıldı. İnkübasyon sonunda 300 bakteri ve 34 mikrofungus türü izole edilerek saflaştırıldı. Hızlı ve etkin bir yöntem olarak önerilen NBRIP-BPB selektif besiyerleri kullanılarak, 24 bakteri ve 6 mikrofungus türünün, trikalsiyum fosfat çözünürlüğünde etkili oldukları kalitatif olarak belirlendi ve bu türlerin tanıları yapıldı

Kaynakça

  • Alagawadi, A.R., Gaur, A.C. 1992, Inoculation of Azospirillum brasilense and phosphate solubilizing bacteria on yield of sorghum [Sorghum bicolor(L.) Moench] in dry Land, Tropical Agriculture, 69; 347-350.
  • Algur, Ö.F., 1992, Temel Biyoteknoloji Ders Notları., s. 77-100 Erzurum.
  • Asea, P.E.A., Kucey R.M.N., Stuart, J.W. 1998, Inorganic phosphate solubilization by two Penicillium species in solution culture and soil, Soil Biology and Biochemistry, 20: 459-464.
  • Beever, R.E., Burns, DJV., 1986, Phosphorus uptake, storage and utilization by fungi, Advances in Botanical Research, 8:127-219.
  • Belimov, A.A., Kojemiakov A.P., Chuvarliyeva, C.V., 1995, Interaction between barley and mixed cultures of nitrogen fixing and phosphate - solubilizing bacteria, Plant and Soil, 173:29-37
  • Bosecker, K., 1997, Bioleaching: metal solubilization by microorganisms. FEMS Microbiology Reviews, 20: 591-604.
  • Gaur, A.C., Ostwal, K.P. 1972, Influence of phosphate dissolving Bacilli on yield and phosphate uptake of wheat crop. Indian Journal of Experimental Biology, 10: 393-394.
  • Glick, B.R. 1995, The enhancement of plant growth by free living bacteria, Canadian Journal of Microbiology, 41: 109-117.
  • Goldstein, A.H., 1986, Bacterial solubilization of mineral phosphates: historical perspective and future prospects, American Journal of Alternative Agriculture, 1:51-57.
  • Goldstein, A.H., Rogers, R.D., Mead, G. 1993, Mining by microbe, Bioresource Technology, 11, 1250-1254.
  • Gyaneshwar, P., Kumar, G.N., Parekh, L.J., Poole, P.S., 2002, Role of soil microorganisms in improving P nutrition of plants. Plant and Soil, 245: 83–93
  • Haider, A.K., Mishra, A.K., Bhattacharyya, P., Chakrabartty, P.K., 1990, Solubilization of rock phosphate by Rhizobium and Bradyrhizobium. Journal of General Applied Microbiology, 36:81-92.
  • Haider, A.K., Chakrabartty, P.K., 1993, Solubilization of inorganic phosphate by Rhizobium. Folia Microbiologica, 38:325-330.
  • Hasenekoğlu, İ., 1991, Toprak Mikrofungusları, 1-7 Cilt. Atatürk Üniversitesi Yayınları, No: 689, Erzurum.
  • Illmer, P., Schinner, F., 1992, Solubilization of inorganic phosphates by microorganisms isolated from forest soil, Soil Biology and Biochemistry, 24: 389-395.
  • Johri, J.K., Surange, S., Nautiyal, C.S., 1999, Occurence of salt, pH and temperature – tolerant, phosphate - solubilizing bacteria in alkaline soils. Current Microbiology, 39: 89-93.
  • Jones, D.A., Smith, B.F.L. Wilson, M.J., Goodman, B.A., 1991, Solubilizator fungi of phosphate in rise soil. Mycological Research, 95:1090-1093.
  • Kadıoğlu, A., 1998. Trabzon., Bitki Fizyolojisi., s. 82-83.
  • Katznelson, H., Peterson, E., Rouatt, J.W., 1962, Phosphate dissolving microorganisms on seed and in the root zone of plants, Canadian Journal of Botany, 40: 1181-1186.
  • Kloepper, J.W., Scroth, M..N., 1978, Plant growth-promoting rhizobacteria on radishes. In: Station dePathologie vegetale et Phyto-bacteriologie, editor. Proceedings of the 4th International Conference on Plant Pathogenic Bacteria Vol II. Tours: Gilbert- Clary, pp. 879-882.
  • Kloepper, J.W., Lifshitz, K., Schroth, M.N., 1988, Pseudomonas inoculants to benefit plant production. ISI Atlas of Science, Animal and Plant Sciences, pp. 60-64.
  • Kloepper, J.W., Lifshitz, K., Zablotowicz, R.M., 1989, Free-living bacterial inocula for enhancing crop productivity. Trends in Biotechnology, 7: 39-43.
  • Kotan, R., 2002, Doğu Anadolu Bölgesinde yetiştirilen yumuşak çekirdekli meyve ağaçlarından izole edilen patojenik ve saprofotik bakteriyel organizmaların klasik ve moleküler metotlar ile tanısı ve biyolojik mücadele imkanlarının araştırılması (Doktora Tezi, Atatürk Üniversitesi Fen Bilimleri Enstitüsü, sayfa, 57-60), Erzurum.
  • Kucey, R.M.N., Janzen, H.H., Leggett, M.E., 1989, Microbially mediated increases in plant- available phosphorus, Advances in Agronomy, 42:199-228.
  • Kundu, B.S., Gaur, A.C., 1984, Rice responce to inoculation with N2 fixing and solubilizing microorganisms, Plant and Soil, 79:227-234.
  • Leyval, C., Barhelin, J., 1989, Interactions between Laccaria laccata, Agrobacterium radiobacter and beech roots: Influence on P, K, Mg and Fe mobilization from mineral and plant growth. Plant and Soil, 17: 103-110.
  • Massimiliano, F., Laura, S., Federico, F., Nikolay, V., 1999, Application of encapsulated Penicillium variable PI6 in solubilization of rock phosphate, Bioresource Technology, 73: 157-162.
  • Mehta, S., Nautiyal, C.S., 2000, An efficient method for qualitative screening of phosphate- solubilizing bacteria, Current Microbiology, 43: 51-56.
  • Nahas, E., 1996, Factors determining rock phosphate solubilization by microorganisms isolated from soil, World Journal of Microbiology and Biotechnology, 12:567-572.
  • Nautiyal, C.S., 1999, An efficient microbiological growth medium for screening phosphate solubilizing microorganisms, FEMS Microbiology Letters, 170: 265-270.
  • Nautiyal, C.S., Bhadauria, S., Kumar, P., Lai, H., Mondal, R., Verma, D., 2000, Stres induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiology Letters, 182: 291-296.
  • Piccini, D., Azcon R., 1987, Effect of phosphate-solubilizing bacteria and versicular mycorrhizal (VAM) on the utilization of bayoran rock phosphate by alfalfa plants using a sand-vermiculite medium, Plant and Soil, 101:45-50.
  • Ray, J., Bagyaraj, D.J., Manjunath, A., 1981, Influence of soil inoculation with versicular arbuscular mycorrizal (VAM) and a phosphate dissolving bacteria on plant growth and 32 P uptake, Soil Biology and Biochemistry, 13:105-108.
  • Reddy, M.S., Kumar, S., Babita, K., Reddy, M.S., 2002, Biosolubilization of poorly soluble rock phosphates by Aspergillus tubingensis and Aspergillus niger, Bioresource Technology, 84: 187-189.
  • Rodriguez, H., Fraga, R., 1999, Phosphate solubilizing bacteria and their role in plant growth promotion, Biotechnolgy Advances, 17: 319-339.
  • Toro, M., Azcon, R., Barea, J.M., 1997, Improvement of arbuscular mycorrhiza development by inoculation of soil with phosphate - solubilizing rhizobacteria to improve rock - phosphate bioavailability (32P) and nutrient cycling, Applied and Environmental Microbiology, 63:4408-4412.
  • Vessey, J.K., 2003, Plant growth promoting rhizobacteria as biofertilizers, Plant and Soil 255: 571–586
  • Yadav, K.S., Dadarwal, K.R., 1997, Phosphate solubilization and mobilization through soil microorganisms. In: Dadarwal KR (ed) : Biotechnological approaches in soil microorganisms for sustainable crop production, Jodhpur, India: Scientific Publishers, pp 293-308.

Isolation and Characterisation of Some Bacteria and Microfungus Solving Tricalcium Phosphate

Yıl 2013, Cilt: 1 Sayı: 1, 11 - 20, 01.06.2013

Öz

In this study some plants species roots and rhizospher soils were collected from different habitats have different temperature, pH, salinity properties etc. From these samples were prepared root extracts and soil dilutions. After that it was inoculated to general mediums Nutrient agar, Potato dextrose agar prepared for bacteria and microfungi. After the incubation, 300 bacteria species and 34 microfungi species were isolated and purified from these samples. The ability of inorganic phosphate solubulisation bacteria and microfungy were tested using an effective and rapid method NBRIP-BPB selective medium quantitively. 24 bacteria and 6 microfungi species were determined and identified having ability of solubilization of tricalcium phosphate effectively

Kaynakça

  • Alagawadi, A.R., Gaur, A.C. 1992, Inoculation of Azospirillum brasilense and phosphate solubilizing bacteria on yield of sorghum [Sorghum bicolor(L.) Moench] in dry Land, Tropical Agriculture, 69; 347-350.
  • Algur, Ö.F., 1992, Temel Biyoteknoloji Ders Notları., s. 77-100 Erzurum.
  • Asea, P.E.A., Kucey R.M.N., Stuart, J.W. 1998, Inorganic phosphate solubilization by two Penicillium species in solution culture and soil, Soil Biology and Biochemistry, 20: 459-464.
  • Beever, R.E., Burns, DJV., 1986, Phosphorus uptake, storage and utilization by fungi, Advances in Botanical Research, 8:127-219.
  • Belimov, A.A., Kojemiakov A.P., Chuvarliyeva, C.V., 1995, Interaction between barley and mixed cultures of nitrogen fixing and phosphate - solubilizing bacteria, Plant and Soil, 173:29-37
  • Bosecker, K., 1997, Bioleaching: metal solubilization by microorganisms. FEMS Microbiology Reviews, 20: 591-604.
  • Gaur, A.C., Ostwal, K.P. 1972, Influence of phosphate dissolving Bacilli on yield and phosphate uptake of wheat crop. Indian Journal of Experimental Biology, 10: 393-394.
  • Glick, B.R. 1995, The enhancement of plant growth by free living bacteria, Canadian Journal of Microbiology, 41: 109-117.
  • Goldstein, A.H., 1986, Bacterial solubilization of mineral phosphates: historical perspective and future prospects, American Journal of Alternative Agriculture, 1:51-57.
  • Goldstein, A.H., Rogers, R.D., Mead, G. 1993, Mining by microbe, Bioresource Technology, 11, 1250-1254.
  • Gyaneshwar, P., Kumar, G.N., Parekh, L.J., Poole, P.S., 2002, Role of soil microorganisms in improving P nutrition of plants. Plant and Soil, 245: 83–93
  • Haider, A.K., Mishra, A.K., Bhattacharyya, P., Chakrabartty, P.K., 1990, Solubilization of rock phosphate by Rhizobium and Bradyrhizobium. Journal of General Applied Microbiology, 36:81-92.
  • Haider, A.K., Chakrabartty, P.K., 1993, Solubilization of inorganic phosphate by Rhizobium. Folia Microbiologica, 38:325-330.
  • Hasenekoğlu, İ., 1991, Toprak Mikrofungusları, 1-7 Cilt. Atatürk Üniversitesi Yayınları, No: 689, Erzurum.
  • Illmer, P., Schinner, F., 1992, Solubilization of inorganic phosphates by microorganisms isolated from forest soil, Soil Biology and Biochemistry, 24: 389-395.
  • Johri, J.K., Surange, S., Nautiyal, C.S., 1999, Occurence of salt, pH and temperature – tolerant, phosphate - solubilizing bacteria in alkaline soils. Current Microbiology, 39: 89-93.
  • Jones, D.A., Smith, B.F.L. Wilson, M.J., Goodman, B.A., 1991, Solubilizator fungi of phosphate in rise soil. Mycological Research, 95:1090-1093.
  • Kadıoğlu, A., 1998. Trabzon., Bitki Fizyolojisi., s. 82-83.
  • Katznelson, H., Peterson, E., Rouatt, J.W., 1962, Phosphate dissolving microorganisms on seed and in the root zone of plants, Canadian Journal of Botany, 40: 1181-1186.
  • Kloepper, J.W., Scroth, M..N., 1978, Plant growth-promoting rhizobacteria on radishes. In: Station dePathologie vegetale et Phyto-bacteriologie, editor. Proceedings of the 4th International Conference on Plant Pathogenic Bacteria Vol II. Tours: Gilbert- Clary, pp. 879-882.
  • Kloepper, J.W., Lifshitz, K., Schroth, M.N., 1988, Pseudomonas inoculants to benefit plant production. ISI Atlas of Science, Animal and Plant Sciences, pp. 60-64.
  • Kloepper, J.W., Lifshitz, K., Zablotowicz, R.M., 1989, Free-living bacterial inocula for enhancing crop productivity. Trends in Biotechnology, 7: 39-43.
  • Kotan, R., 2002, Doğu Anadolu Bölgesinde yetiştirilen yumuşak çekirdekli meyve ağaçlarından izole edilen patojenik ve saprofotik bakteriyel organizmaların klasik ve moleküler metotlar ile tanısı ve biyolojik mücadele imkanlarının araştırılması (Doktora Tezi, Atatürk Üniversitesi Fen Bilimleri Enstitüsü, sayfa, 57-60), Erzurum.
  • Kucey, R.M.N., Janzen, H.H., Leggett, M.E., 1989, Microbially mediated increases in plant- available phosphorus, Advances in Agronomy, 42:199-228.
  • Kundu, B.S., Gaur, A.C., 1984, Rice responce to inoculation with N2 fixing and solubilizing microorganisms, Plant and Soil, 79:227-234.
  • Leyval, C., Barhelin, J., 1989, Interactions between Laccaria laccata, Agrobacterium radiobacter and beech roots: Influence on P, K, Mg and Fe mobilization from mineral and plant growth. Plant and Soil, 17: 103-110.
  • Massimiliano, F., Laura, S., Federico, F., Nikolay, V., 1999, Application of encapsulated Penicillium variable PI6 in solubilization of rock phosphate, Bioresource Technology, 73: 157-162.
  • Mehta, S., Nautiyal, C.S., 2000, An efficient method for qualitative screening of phosphate- solubilizing bacteria, Current Microbiology, 43: 51-56.
  • Nahas, E., 1996, Factors determining rock phosphate solubilization by microorganisms isolated from soil, World Journal of Microbiology and Biotechnology, 12:567-572.
  • Nautiyal, C.S., 1999, An efficient microbiological growth medium for screening phosphate solubilizing microorganisms, FEMS Microbiology Letters, 170: 265-270.
  • Nautiyal, C.S., Bhadauria, S., Kumar, P., Lai, H., Mondal, R., Verma, D., 2000, Stres induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiology Letters, 182: 291-296.
  • Piccini, D., Azcon R., 1987, Effect of phosphate-solubilizing bacteria and versicular mycorrhizal (VAM) on the utilization of bayoran rock phosphate by alfalfa plants using a sand-vermiculite medium, Plant and Soil, 101:45-50.
  • Ray, J., Bagyaraj, D.J., Manjunath, A., 1981, Influence of soil inoculation with versicular arbuscular mycorrizal (VAM) and a phosphate dissolving bacteria on plant growth and 32 P uptake, Soil Biology and Biochemistry, 13:105-108.
  • Reddy, M.S., Kumar, S., Babita, K., Reddy, M.S., 2002, Biosolubilization of poorly soluble rock phosphates by Aspergillus tubingensis and Aspergillus niger, Bioresource Technology, 84: 187-189.
  • Rodriguez, H., Fraga, R., 1999, Phosphate solubilizing bacteria and their role in plant growth promotion, Biotechnolgy Advances, 17: 319-339.
  • Toro, M., Azcon, R., Barea, J.M., 1997, Improvement of arbuscular mycorrhiza development by inoculation of soil with phosphate - solubilizing rhizobacteria to improve rock - phosphate bioavailability (32P) and nutrient cycling, Applied and Environmental Microbiology, 63:4408-4412.
  • Vessey, J.K., 2003, Plant growth promoting rhizobacteria as biofertilizers, Plant and Soil 255: 571–586
  • Yadav, K.S., Dadarwal, K.R., 1997, Phosphate solubilization and mobilization through soil microorganisms. In: Dadarwal KR (ed) : Biotechnological approaches in soil microorganisms for sustainable crop production, Jodhpur, India: Scientific Publishers, pp 293-308.
Toplam 38 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Research Article
Yazarlar

Mehmet Nuri Aydoğan Bu kişi benim

Ömer Faruk Algur Bu kişi benim

Mustafa Özdemir Bu kişi benim

Yayımlanma Tarihi 1 Haziran 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 1 Sayı: 1

Kaynak Göster

APA Aydoğan, M. N., Algur, Ö. F., & Özdemir, M. (2013). Trikalsiyum Fosfat Çözebilen Bazı Bakteri ve Mikrofungusların İzolasyonu ve Karakterizasyonu. ADYUTAYAM Dergisi, 1(1), 11-20.