Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇

Abstract

ÖZET

Amaç:Antioksidan özelliği bilinen quercitrinin deneysel intestinal iskemi reperfüzyon (İ-R) modelinde reperfüzyon hasarı üzerine etkilerini araştırmak.

Araçlar ve Yöntem : Çalışmada 30 adet Wistar–Albino sıçan kullanıldı. Sham grubu (n=10), İ-R (kontrol) grubu (n=10) ve quercitrin+iskemi-reperfüzyon (Q+İ-R) grubu (n=10) olmak üzere 3 grup oluşturuldu. Sham grubundaki sıçanlar laparotomiyi takiben 3 saat gözlem sonrası sakrifiye edildi. İ-R grubuna 1 saat iskemi ve takiben 2 saat reperfüzyon uygulandı. Q+İ-Rgrubuna 24 saat önce gastrik gavaj ile 5 mg/kg dozunda quercitrin verildi. Histopatolojik inceleme ve doku malondialdehit (MDA), süperoksiddismutaz (SOD), glutatyonperoksidaz (GSH-Px), nitrik oksit (NO) ve 8-hidroksi-deoksiguanozin (8-OHDG) aktivitesi ölçümü için ileum rezeksiyonu yapıldıktan sonra sıçanlar sakrifiye edildi.

Bulgular:Histopatolojik olarak Q+İ-R ve İ-R grupları arasındaki fark anlamlı bulundu (p<0.001). Q+İ-R grubunda MDA, NO ve 8-OHDG aktivitesinde azalış istatistiksel olarak anlamlıydı (p<0,001). GSH-Px ve SOD aktivitesinde İ-R göre daha fazla azalmakta idi ve sham grubuna göre istatistiksel olarak anlamlıydı. (p<0,001).

Sonuç:Quercitrinin İ-R ile oluşan oksidatif stresi önlemiş ancak vücudun doğal oksidatif stres koruyucuları üzerinde etki yapmamıştı.

Keywords

• İskemi,reperfüzyon,quercitrin,ince barsak

The Effects Of Quercitrin In Experimental Rat Model Of Intestinal Ischemia-Reperfusion

Abstract

ABSTRACT

Objective: To investigate effects of quercitrin known for its antioxidant features on experimental model of intestinal ischemia-reperfusion (I-R).

Material and method: Thirty Wistar-Albino rats were used in the study. Rats were assigned into 3 groups including sham group (n=30), I-R group (n=30) and quercitrin plus I-R group (n=10). In the control group, rats were sacrificed after 3-hours after laparotomy. In ischemia-reperfusion groups, rats underwent one hour of ischemia followed by 2 hours of reperfusion. In quercitrin plus reperfusion group, 5 mg/kg quercitrin was given to rats 24 hours before laparotomy by gastric lavage. Rats were sacrificed after resection of ileum for histopathological examination and measurements of tissue malonyldialdehyde (MDA), super oxide dismutase (SOD), glutathione peroxidase (GHS-PX), nitric oxide (NO) and 8-hydroxydeoxyguanosine (8-oHDG) activities.

Results: Significant differences were detected in histopathological examination between quercitrin plus I-R and I-R groups (p<0.001). MDA, NO and 8-oHDG activities were significantly decreased in quercitrin plus I-R group (p<0.001). The extent of decrease in GSH-Px and SOD activities was greater when compared to I-R group while it was significantly greater when compared to sham group (p<0.001).

Conclusion: Quercitrin prevented oxidative stress induced by I-R but had no effect on native defenders against oxidative stress.

Keywords

• Ischemia,reperfusion,quercitrin,small intestine

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