Geranium sp’nin Antimikrobiyal ve Anti-quorum sensing aktivitesi

Öz

Amaç: Son yıllarda hızla artan antibiyotik direnci, halk sağlığı tehditlerinin başında gelmektedir. Dirençle mücadelede etkili antibiyotikler için alternatif yöntemler araştırılmaktadır. Sardunya türleri kabızlık, sindirim bozuklukları ve diyabet için yaygın olarak kullanılmaktadır. Aynı zamanda bitkinin çeşitli halk sağlığı uygulamalarında kullanıldığı bilinmektedir. Bu bilgiler doğrultusunda Geranium sp bitkisinin antimikrobiyal ve anti-qorum sensing özelliklerinin araştırılması amaçlanmıştır. Gereç ve yöntemler: Bu çalışmada, Rize iline ait Geranium sp. çiçek kısımlarının metanol, etil asetat, etanol ve hekzan özütlerinin hazırlanması ve agar kuyu difüzyon yöntemi ile çeşitli Gram-negatif ve Gram-pozitif bakterilere ve iki mantar türüne karşı antimikrobiyal aktivitelerinin araştırılması amaçlanmıştır. Chromobacterium violaceum suşu kullanılarak anti-quorum algılama aktivitesi belirlenmiştir. Bulgular: Geranium sp.’nin metanol özütü yedi türe karşı antimikrobiyal aktivite gösterirken, etil asetat ile ekstraksiyon sekiz türe karşı antimikrobiyal aktivite göstermiştir. Etanol ile ekstrakte edilen örnekler en düşük antimikrobiyal aktiviteye sahip olduğundan sadece altı tür etkilenmiştir. Ayrıca, Geranium sp.’nin metanol özütü violacein inhibisyon aktivitesine sahiptir. Sonuç: Sonuç olarak, bitkinin özellikle metanol öztünün antimikrobiyal ve anti-quorum algılama özelliklerine sahip olduğu belirlendi.

Anahtar Kelimeler

• viyolasin
• quorum sensing
• Geranium sp

Antimicrobial and Anti-quorum Sensing Activity of Geranium sp.

Öz

Aim: Antibiotic resistance, which has increased rapidly in recent years, is one of the leading public health threats. Alternative methods are being investigated for effective antibiotics in the fight against resistance. Geranium species are widely used for constipation, digestive disorders, and diabetes. It is also known that the plant is used in various public health applications. In line with this information, it was aimed to investigate the antimicrobial and anti-quorum sensing properties of the Geranium sp. Material and Methods: In this study it was aimed to prepare methanol, ethyl acetate, ethanol, and hexane extracts of Geranium sp. flower parts belonging to Rize province and investigate their antimicrobial activities by agar well diffusion method against various Gram-negative and Gram-positive bacteria and two fungal species. Anti-quorum sensing activity was determined using Chromobacterium violaceum strain. Results: Methanol extract of Geranium sp. showed antimicrobial activity against seven species whereas extraction with ethyl acetate showed antimicrobial activity against eight species. The ethanol extracted samples have the lowest antimicrobial activity so that only six species were affected. Additionally, methanol extract of Geranium sp. had violacein inhibition activity. Conclusions: As a result, it was determined that the plant, especially its methanol extract, has antimicrobial and anti-quorum sensing properties.

Anahtar Kelimeler

• violacein
• quorum sensing
• Geranium sp

Kaynakça

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