Antimicrobial and Anti-quorum Sensing Activity of Geranium sp.

Yıl 2024, , 100 - 104, 30.10.2024

Ülkü Zeynep Üreyen Esertaş , Saliha Ekşi

https://doi.org/10.61845/agrimedical.1555075

Öz

Aim: Antibiotic resistance, which has increased rapidly in recent years, is one of the leading public health threats. Alternative methods are being investigated for effective antibiotics in the fight against resistance. Geranium species are widely used for constipation, digestive disorders, and diabetes. It is also known that the plant is used in various public health applications. In line with this information, it was aimed to investigate the antimicrobial and anti-quorum sensing properties of the Geranium sp.
Material and Methods: In this study it was aimed to prepare methanol, ethyl acetate, ethanol, and hexane extracts of Geranium sp. flower parts belonging to Rize province and investigate their antimicrobial activities by agar well diffusion method against various Gram-negative and Gram-positive bacteria and two fungal species. Anti-quorum sensing activity was determined using Chromobacterium violaceum strain.
Results: Methanol extract of Geranium sp. showed antimicrobial activity against seven species whereas extraction with ethyl acetate showed antimicrobial activity against eight species. The ethanol extracted samples have the lowest antimicrobial activity so that only six species were affected. Additionally, methanol extract of Geranium sp. had violacein inhibition activity.
Conclusions: As a result, it was determined that the plant, especially its methanol extract, has antimicrobial and anti-quorum sensing properties.

Anahtar Kelimeler

violacein, quorum sensing, Geranium sp

Proje Numarası

RTEÜ BAP959-2018

Geranium sp’nin Antimikrobiyal ve Anti-quorum sensing aktivitesi

Öz

Amaç: Son yıllarda hızla artan antibiyotik direnci, halk sağlığı tehditlerinin başında gelmektedir. Dirençle mücadelede etkili antibiyotikler için alternatif yöntemler araştırılmaktadır. Sardunya türleri kabızlık, sindirim bozuklukları ve diyabet için yaygın olarak kullanılmaktadır. Aynı zamanda bitkinin çeşitli halk sağlığı uygulamalarında kullanıldığı bilinmektedir. Bu bilgiler doğrultusunda Geranium sp bitkisinin antimikrobiyal ve anti-qorum sensing özelliklerinin araştırılması amaçlanmıştır.
Gereç ve yöntemler: Bu çalışmada, Rize iline ait Geranium sp. çiçek kısımlarının metanol, etil asetat, etanol ve hekzan özütlerinin hazırlanması ve agar kuyu difüzyon yöntemi ile çeşitli Gram-negatif ve Gram-pozitif bakterilere ve iki mantar türüne karşı antimikrobiyal aktivitelerinin araştırılması amaçlanmıştır. Chromobacterium violaceum suşu kullanılarak anti-quorum algılama aktivitesi belirlenmiştir.
Bulgular: Geranium sp.’nin metanol özütü yedi türe karşı antimikrobiyal aktivite gösterirken, etil asetat ile ekstraksiyon sekiz türe karşı antimikrobiyal aktivite göstermiştir. Etanol ile ekstrakte edilen örnekler en düşük antimikrobiyal aktiviteye sahip olduğundan sadece altı tür etkilenmiştir. Ayrıca, Geranium sp.’nin metanol özütü violacein inhibisyon aktivitesine sahiptir.
Sonuç: Sonuç olarak, bitkinin özellikle metanol öztünün antimikrobiyal ve anti-quorum algılama özelliklerine sahip olduğu belirlendi.

Anahtar Kelimeler

viyolasin, quorum sensing, Geranium sp

Etik Beyan

Bu çalışma etik kurul gerektirmemektedir

Destekleyen Kurum

Recep Tayyip Erdoğan Üniversitesi

Proje Numarası

RTEÜ BAP959-2018

Kaynakça

• 1. Machowska A, Stalsby Lundborg C. Drivers of Irrational Use of Antibiotics in Europe. Int J Environ Res Public Health. 2018;16(1):27.
• 2. Hoxha I, Godman B, Malaj A, Meyer JC. 11-Year Trend in Antibiotic Consumption in a South- Eastern European Country; the Situation in Albania and the Implications for the Future. Antibiotics (Basel). 2023;12(5):882.
• 3. Zarauz JM, Zafrilla P, Ballester P, Cerda B. Study of the Drivers of Inappropriate Use of Antibiotics in Community Pharmacy: Request for Antibiotics Without a Prescription, Degree of Adherence to Treatment and Correct Recycling of Leftover Treatment. Infect Drug Resist. 2022;15:6773-83.
• 4. Ginovyan M, Trchounian A. Novel approach to combat antibiotic resistance: evaluation of some Armenian herb crude extracts for their antibiotic modulatory and antiviral properties. J Appl Microbiol. 2019;127(2):472-80.
• 5. Muhammad SR, Abed IJ. Study of the Synergistic Effect of Three Volatile Oils on Antibiotic- Resistant Bacteria Isolated from Burns. The Egyptian Journal of Hospital Medicine. 2023;90(1):1421-9.
• 6. Fields L, Craig WR, Wasileski SA, Wolfe AL. Effects of Shade on Antibacterial Production in Aloe vera Plants: A Model Course Based Undergraduate Research Experience for First-and Second- Year Chemistry and Biochemistry Students. World. 2019;7(4):248-53.
• 7. Esertaş ÜZÜ, Cora M. Biological activities of Elaeagnus umbellata methanol extract. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2024;27(6):1262-8.
• 8. Hanci H, CoŞKun MV, Uyanik MH, Sezen S, İGan H. In vitro Antifungal Activities of Fluconazole, Camellia sinensis and Cydonia oblonga Leaf Extracts Against Candida Species Isolated from Blood Cultures. Bezmialem Science. 2019;7(2):107-12.
• 9. Baytop T. Türkiye’de Bitkiler ile Tedavi, 2. baskı: İstanbul. Nobel Tıp Kitabevi. 1999.
• 10. Adetunji CO, Oyeyemi OT. Antiprotozoal activity of some medicinal plants against Entamoeba histolytica, the causative agent of amoebiasis. Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics: CRC Press; 2022. p. 341-58.
• 11. Karato M, Yamaguchi K, Takei S, Kino T, Yazawa K. Inhibitory effects of pasuchaca (Geranium dielsiaum) extract on alpha-glucosidase in mouse. Biosci Biotech Bioch. 2006;70(6):1482-4.
• 12. Liu QH, Jeong JE, Choi EJ, Moon YH, Woo ER. A new furofuran lignan from Geranium thunbergii Sieb. et Zucc. Arch Pharm Res. 2006;29(12):1109-13.
• 13. Saliha E, Esertaş ÜZÜ, Kilic AO, Ejder N, Uzunok B. Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’inhibition potentials of Castanea sativa Mill. extracts. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2020;48(1):66-78.
• 14. Sezen S, Ertugrul MS, Balpinar O, Bayram C, Ozkaraca M, Okkay IF, et al. Assessment of antimicrobial activity and In Vitro wound healing potential of ZnO nanoparticles synthesized with Capparis spinosa extract. Environ Sci Pollut Res Int. 2023;30(55):117609-23.
• 15. Ureyen Esertas UZ, Kara Y, Kilic AO, Kolayli S. A comparative study of antimicrobial, anti-quorum sensing, anti-biofilm, anti-swarming, and antioxidant activities in flower extracts of pecan (Carya illinoinensis) and chestnut (Castanea sativa). Arch Microbiol. 2022;204(9):589.
• 16. Leclercq R, Canton R, Brown DF, Giske CG, Heisig P, MacGowan AP, et al. EUCAST expert rules in antimicrobial susceptibility testing. Clin Microbiol Infect. 2013;19(2):141-60.
• 17. Parasuraman P, Devadatha B, Sarma VV, Ranganathan S, Ampasala DR, Siddhardha B. Antiquorum sensing and antibiofilm activities of Blastobotrys parvus PPR3 against Pseudomonas aeruginosa PAO1. Microb Pathogenesis. 2020;138:103811.
• 18. Castillo-Juarez I, Garcia-Contreras R, Velazquez-Guadarrama N, Soto-Hernandez M, Martinez- Vazquez M. Amphypterygium adstringens anacardic acid mixture inhibits quorum sensingcontrolled virulence factors of Chromobacterium violaceum and Pseudomonas aeruginosa. Arch Med Res. 2013;44(7):488-94.
• 19. Wijesinghe GK, Feiria SB, Maia FC, Oliveira TR, Joia F, Barbosa JP, et al. In-vitro Antibacterial and Antibiofilm Activity of Cinnamomum verum Leaf Oil against Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae. An Acad Bras Cienc. 2021;93(1):e20201507.
• 20. Alonso AM, Reyes-Maldonado OK, Puebla-Perez AM, Arreola MPG, Velasco-Ramirez SF, Zuniga- Mayo V, et al. GC/MS Analysis, Antioxidant Activity, and Antimicrobial Effect of Pelargonium peltatum (Geraniaceae). Molecules. 2022;27(11):3436.
• 21. Bigos M, Wasiela M, Kalemba D, Sienkiewicz M. Antimicrobial activity of Geranium oil against clinical strains of Staphylococcus aureus. Molecules. 2012;17(9):10276-91.
• 22. Łysakowska ME, Sienkiewicz M, Banaszek K, Sokołowski J. The sensitivity of endodontic Enterococcus spp. strains to Geranium essential oil. Molecules. 2015;20(12):22881-9.
• 23. Asfour HZ. Anti-Quorum Sensing Natural Compounds. J Microsc Ultrastruct. 2018;6(1):1-10.
• 24. Gururani MA, Atteya AK, Elhakem A, El-Sheshtawy ANA, El-Serafy RS. Essential oils prolonged the cut carnation longevity by limiting the xylem blockage and enhancing the physiological and biochemical levels. Plos One. 2023;18(3):e0281717.
• 25. Boukhris M, Bouaziz M, Feki I, Jemai H, El Feki A, Sayadi S. Hypoglycemic and antioxidant effects of leaf essential oil of Pelargonium graveolens L’Hér. in alloxan induced diabetic rats. Lipids in health and disease. 2012;11:1-10.
• 26. Nam HH, Nan L, Choo BK. Dichloromethane Extracts of Geranium Koreanum Kom. Alleviates Esophagus Damage in Acute Reflux Esophagitis-Induced Rats by Anti-Inflammatory Activities. Int J Mol Sci. 2018;19(11):3622.
• 27. Androutsopoulou C, Christopoulou SD, Hahalis P, Kotsalou C, Lamari FN, Vantarakis A. Evaluation of Essential Oils and Extracts of Rose Geranium and Rose Petals as Natural Preservatives in Terms of Toxicity, Antimicrobial, and Antiviral Activity. Pathogens. 2021;10(4):494.
• 28. Mehran M, Hosseini H, Akbari H, Hatami A, Safaei A. Investigation of the Effects of Chemical, Organic and Compost Fertilizers on Chemical Composition of Essential Oil of Pelargonium graveolens. Journal Of Horticultural Science. 2020;34(3):417-26.
• 29. Kalia VC, Patel SKS, Kang YC, Lee JK. Quorum sensing inhibitors as antipathogens: biotechnological applications. Biotechnol Adv. 2019;37(1):68-90.
• 30. Esertaş ÜZÜ, Durukan İ, Kılıç AO, Ekşi S. Determination of antimicrobial and quorum sensing inhibition potentials of different types of berries from Rize. Kastamonu University Journal of Forestry Faculty. 2024;24(1):74-80.
• 31. Elmanama AA, Al-Reefi MR. Antimicrobial, anti-biofilm, anti-quorum sensing, antifungal and synergistic effects of some medicinal plants extracts. IUG Journal of Natural Studies. 2017.
• 32. Abdel Bar FM, Alossaimi MA, Elekhnawy E, Alzeer MAA, Abo Kamer A, Moglad E, ElNaggar MH. Anti-Quorum Sensing and Anti-Biofilm Activity of Pelargonium x hortorum Root Extract against Pseudomonas aeruginosa: Combinatorial Effect of Catechin and Gallic Acid. Molecules. 2022;27(22):7841.