Determination of the Effect of Indole Acetic Acid (IAA) Produced from Edible Mushrooms on Plant Growth and Development

Abstract

In this study; from edible mushrooms Pleurotus eryngii, Pleurotus ostreatus, Pleurotus florida, Pleurotus sajor-caju and Pleurtotus citrinopleatus species IAA production amounts were determined and the obtained IAAs were obtained from bread wheat Triticum aestivum cv. Kirik. Its effect on fracture growth and development was investigated. The plants were grown at 22/20 oC for a total of 15 days. IAAs (1g/L) were applied to the roots on the applied and were harvested on the 15th day to be used as experimental material. Then, root-stem length, fresh-dry weight, protein, chlorophyll and sugar contents were determined. As a result of the measurements, it was observed that IAAs belonging to the genus Pleurotus applied exogenously affected plant growth and development positively.

Keywords

• IAA
• Pleurotus
• Plant Growth
• Tiriticum aestivum

Kaynakça

1. [1] Soliman, N. A., Al-amin, S. M., Mesbah, A. E., Ibrahim, A. M., & Mahmoud, A. M. Pathogenicity of three entomopathogenic fungi against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann)(Diptera: Tephritidae). Egyptian Journal of Biological Pest Control, 2020. 30(1), 1-8.
2. [2] Kumar, N.V.,Rajam, S. and Rani, M.E. Plant Growth Promotion Efficacy of Indole Acetic Acid (IAA) Produced by a Mangrove Associated Fungi-Trichoderma viride VKF3. International Journal of Current Microbiology and Applied Sciences 2017. 6(11): p. 2692-2701.
3. [3] Jatav, H. S., Jayant, H., Kumar, S., Kumar, V., Chattopadhya, A., Dhawal, S. K., & Singh, Y. V. Role of Biochar: In agriculture sector its implication and perspective. Int. J. Chem. Stud, 2017. 5, 14-18.
4. [4] Larekeng, S. H., Restu, M., Tunggal, A., & Susilowati, A. Isolation and identification of rhizospheric fungus under Mahoni (Swietenia mahagoni) stands and its ability to produce IAA (Indole Acetid Acid) hormones. In IOP Conference Series: Earth and Environmental Science 2019. (Vol. 343, No. 1, p. 012051). IOP Publishing.
5. [5] Wahyuni, D., Linda, T. M., & Lestari, W. Potensi Isolat Bakteri Pelarut Fosfat Asal Tanah Gambut Riau dalam Memproduksi Hormon Indole Acetic Acid (IAA) dan Pengaruhnya Terhadap Perkecambahan Benih Cabai Merah (Capsicum annuum L.). BIO-SITE| Biologi dan Sains Terapan, 2016. 2(2).
6. [6] Huang XF, Chaparro JM, Reardon KF, Zhang R, Shen Q, Rhizosphere ŝnƚĞrĂcƟŽnƐ͗ root exudates, microbes, and microbial cŽmmƵnŝƟĞƐ͘ Botany 2014. 92: 267-275.
7. [7] Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 1976. 72(1-2), 248-254.
8. [8] Lichtenthaler, H. K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in enzymology, 1987. 148, 350-382.

9. [9] Mehmood, A., Hussain, A., Irshad, M., Khan, N., Hamayun, M., Ismail, Lee, I. J. IAA and flavonoids modulates the association between maize roots and phytostimulant endophytic Aspergillus fumigatus greenish. Journal of plant interactions, 2018. 13(1), 532-542.
10. [10] Fouda, A. H., Hassan, S. E. D., Eid, A. M., & Ewais, E. E. D.. Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.). Annals of Agricultural Sciences, 2015. 60(1), 95-104.
11. [11] Kaya C, Ashraf M, Dikilitas M, Tuna AL Alleviation of salt stressinduced adverse effects on maize plants by exogenous application of indoleacetic acid (IAA) and inorganic nutrients-a field trial. Aust J Crop Sci 2013. 7:249.
12. [12] Nieto-Jacobo, M.F., J.M. Steyaert, F.B. Salazar-Badillo, D.V. Nguyen, M. Rostás, M. Braithwaite, J.T. De Souza, J.F. Jimenez-Bremont, M. Ohkura, A. Stewart, andA. Mendoza-Mendoza. Environmental growth conditions of Trichoderma spp. affects indole acetic acid derivatives, volatile organic compounds, and plant growth promotion. Frontiers in plant science 2017. 8.
13. [13] Biesaga-Kościelniak J, Kościelniak J, Filek M, Marcińska I, Krekule J, Macháčková I. The effect of plant growth regulators and their interaction with electric current on winter wheat development. Acta physiologiae plantarum. 2010. 32(5):987-995.
14. [14] Kumar, V., Kushwaha, S. P. Exogenously applied plant growth regulators enhanced the yield and quality attributes of wheat (Triticum aestivum L.). IJCS, 2020. 8(6), 467-469.
15. [15] Vamil, R., Haq, A., & Agnihotri, R. K Plant growth regulators as effective tool for germination and seedling growth for Bambusa arundinaceae. Research Journal of Agricultural Science, 2010. 1(1), 233–236.
16. [16] Khandaker, M. M., Azam, H. M., Rosnah, J., Tahir, D., & Nashriyah, M. The Effects of Application of Exogenous IAA and GA3 on the Physiological Activities and Quality of Abelmoschus esculentus (Okra) var. Singa 979. Pertanika Journal of Tropical Agricultural Science, 2018. 41(1).
17. [17] Zhao, T., Deng, X., Xiao, Q., Han, Y., Zhu, S., & Chen, J. IAA priming improves the germination and seedling growth in cotton (Gossypium hirsutum L.) via regulating the endogenous phytohormones and enhancing the sucrose metabolism. Industrial Crops and Products, 2020. 155, 112788.
18. [18] Mirheidari, F., Hatami, M., & Ghorbanpour, M. Effect of different concentrations of IAA, GA3 and chitosan nano-fiber on physio-morphological characteristics and metabolite contents in roselle (Hibiscus sabdariffa L.). South African Journal of Botany. 2021.