A Comparative Analysis of Biofilm Characteristics of Dual-Species Periodontopathogenic Biofilm based on Fusobacterium nucleatum and the Dual- Species Biofilm Response in the Presence of Antimicrobial Peptide

Öz

Fusobacterium nucleatum (F.nucleatum), which acts as a linking periodontopathogenic bacteria, has the ability of coaggregation with early and late colonization species. It is the most intense gram-negative bacteria found in periodontal health and increases in periodontal diseased areas numerically. In this study, comparative analyses of dual and mono-biofilm structural characteristics formed by Porphyromonas gingivalis and Prevotella intermedia with F. nucleatum have been evaluated. In all results, more biofilm formation was observed in dual-species compared to mono-species biofilms. Biofilm formation of F. nucleatum-P.intermedia has increased in the presence of Human Neutrophilic Peptide (HNP-1). In this study it is concluded that dual-periodontopathogenic coaggregations based on F. nucleatum could produce more intense biofilm structure than single-species ones, and the increase in dual-species biofilm mass in the presence of low doses of HNP-1 may be a defense mechanism of dual periodontopathogenic biofilm.

Anahtar Kelimeler

• Periodontitis,subgingival,biofilm,antimicrobial,peptide,gram-negative bacteria,defensin,inflammation

Kaynakça

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