Identification of diversity and relationships of grapefruit (Citrus paradisi) and pummelo (Citrus maxima) accessions by using SSR molecular markers

Yıl 2012, Cilt: 25 Sayı: 1, 1 - 7, 01.06.2012

İ. Polat E. Turgutoğlu

Öz

In this study, genetic relationship and diversity were determined by SSR (simple sequence repeat) markers among thirty grapefruits (Citrus paradisi Macf.), one Citrus hassaku and five pummello [(Citrus maxima (Burm.) Merr.)] accessions derived from selections and introduction. Of the 26 SSR primers used produced fifteen polymorphic fragments. Genetic relationship and distance were determined by using UPGMA (unweighted-pair group method arithmetic average) dendrogram and PCA (principal component analysis) analysis. The Dice (1945)’s similarity coefficient among grapefruit and pummello accessions ranged from 0.60 to 0.97 and matrix correlation (r) was 0.88. The analyses showed that there was 97% genetic similarity in terms of primers investigated between Foster B 6/5 28-12 and Ray Ruby grapefruit. On the contrary, Red Şadok with 60% of genetic similarity was the farthest among 36 accessions. All pummelos were took place within one group. But, some grapefruits also nested in the pummelos group. This result may be due to the grapefruits are natural hybrid between pummelo and sweet orange. Genetic variation was quite low in some grapefruit accessions. Similarity-based analyses supported the theory of grapefruits are of nucellar origin.

Anahtar Kelimeler

Citrus paradisi, Citrus maxima, SSR, Genetic relationship, Genetic distinguish

Bazı altıntop (Citrus paradisi) ve adak şadoklarda (Citrus maxima) genetik akrabalık ve farklılıklarının SSR markırlarıyla tanımlanması

Öz

Seleksiyon ve introduksiyon yoluyla elde edilmiş 30 adet altıntop (Citrus paradisi Macf.), 1
adet Citrus hassaku ve 5 adet şadok [Citrus maxima (Burm.) Merr.)]‟un genetik farklılığı ve
birbiriyle olan genetik yakınlığını belirlemek amacıyla SSR (simple sequence repeat) moleküler
markır tekniği kullanılmıştır. Çalışmada kullanılan 26 adet SSR primerinden 15 tanesi
polimorfizm sağlamıştır. UPGMA (unweighted-pair group method aritmetic average)
dendrogram ve PCA (principal component analysis) analizleri sonucu bireylerin birbirleriyle
olan genetik yakınlıkları ve uzaklıkları belirlenmiştir. Dice (1945)‟ın benzerlik katsayısına göre
benzerlik oranları 0,60-0,97 arasında değişim göstermiş, matrix korelosyonu (r) 0,88 olarak
bulunmuştur. Değerlendirme sonucunda, Foster B 6/5 28-12 ile Ray Ruby altıntopu arasında
incelenen primerlere göre % 97 oranında bir benzerlik olduğu saptanmış, Red Şadok ise % 60
oranıyla en uzak bireyi oluşturmuştur. Çalışmada kullanılan tüm şadoklar bir grup içerisinde
yer almıştır. Buna karşın şadok grubu içerisinde bazı altıntopların da yer aldığı belirlenmiştir.
Bu durumun altıntopların şadok ile portakal melezi olmasından kaynaklanabileceği
değerlendirilmiştir. Bazı altıntoplarda ise genetik yakınlık oldukça fazladır. Düşük varyasyon
göstermesinin sebebi, altıntopların mutasyon orijinli olmasından kaynaklanabilir.

Anahtar Kelimeler

Citrus paradisi, Citrus maxima, SSR, Genetik akrabalık, Genetik farklılık

Kaynakça

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