Araştırma Makalesi
BibTex RIS Kaynak Göster

A Study on Adventitious Shoot Formation from Stem and Petiole Explants of Grape (Vitis vinifera L.)

Yıl 2008, Cilt: 21 Sayı: 2, 231 - 240, 01.12.2008

Öz

In this study, the effects of explant type, nutrient media and culture conditions on in vitro regeneration of stem and petiole explants taken from in vitro plants of Kalecik karası were investigated. The explants were planted on MS and NN medium supplemented with different concentrations of BAP, 2,4-D, zeatin, IBA and casein hydrolisate and then they were cultured in dark or light conditions. As a result of this research, formation of callus, direct and indirect adventitious shoot formation were obtained in different ratio according to explant type, nutrient media and culture conditions. Stem explants gave the best results for the direct and indirect adventitious shoot formation. While formation of callus were obtained from MS medium added 0.2 mg/l BAP+1 mg/l 2,4-D+2.5 mg/l IBA and NN medium added 2 mg/l 2,4-D the highest direct adventitious shoot formation were obtained from MS medium added 2 mg/l zeatin and NN medium added 2 mg/l BAP and indirect adventitious shoot formation were obtained from MS medium added 0.2 mg/l BAP+1 mg/l 2,4-D+1 g/l casein hydrolisate and NN medium contained 2 mg/l zeatin. Dark condition was showed positive effect on formation of callus. On the other hand, 16/8 h light regime increased direct and indirect adventitious shoot formation.

Kaynakça

  • Bayır A., Uzun, H. İ. ve Elidemir, A.Y., 2004. Effects of 2,4-D, Kinetin and BAP Combinations on Callus Formation and Organogenesis in Vitis. The 14th FESPB Congress August 23-27, 2004, Cracow/Poland. Acta Physiologiae Plantarum, 26(3), 255.
  • Carimi, F., Barizza, E., Gardiman, M. and Schiavo, F.L., 2005. Somatic Embryogenesis from Stigmas and Styles of Grapevine. In Vitro Cellular and Developmental Biology Plant, 41(3), 249-252.
  • Cheng, Z.M. and Reisch, B.I., 1989. Shoot Regeneration from Petioles and Leaves of Vitis labruscana Catawba. Plant Cell Reports, 8(7), 403-406.
  • Clog, E., Bass, P. and Walter, B., 1990. Plant Regeneration by Organogenesis in Vitis Rootstock Species. Plant Cell Reports, 8(12), 726-728.
  • Goebel Tourand, I., Mauro, M.C., Sossountzov, L., Miginiac, E. and Deloire, A., 1993. Arrest of Somatic Embryo Development in Grapevine: Histological Characterization and The Effect of ABA, BAP and Zeatin in Stimulating Plantlet Development. Plant Cell, Tissue and Organ Culture, 33, 91-103.
  • Emershad, R.L. and Ramming, D.W., 1994. Somatic Embryogenesis and Plant
  • Development from Immature Zygotic Embryos of Seedless Grapes (Vitis vinifera L.). Plant Cell Reports, 14(1), 6-12.
  • Göktürk Baydar, N., 1997. Bağcılıkta In Vitro Mikroaşılama Tekniği ile Çoğaltma Üzerinde Araştırmalar. Ankara Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, 99 s., Ankara.
  • Göktürk Baydar, N., 2000. Asmada (Vitis spp.) Yapraklardan Adventif Sürgün Oluşumu Üzerine Bir Araştırma. Turk J. Biol., 24, 645-656.
  • Göktürk Baydar, N. ve Çetin, S., 2003. Asma Yaprak Eksplantlarının In Vitro Gelişmeleri Üzerine Genotip, Eksplant Tipi ve Besin Ortamlarının Etkileri. 13. Biyoteknoloji Kongresi Bildiriler Kitabı, 99-104, Çanakkale.
  • Gök Tangolar, S. 2002. Asmalarda Somatik Embriyogenesis ve Organogenesis Yoluyla Bitki Elde Edilmesi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, 165 s., Adana.
  • Jayasankar, S., Gray, D.J. and Litz R.Z., 1999. High Efficiency Somatic Embryogenesis and Plant Regeneration from Suspension Cultures of Grapevine. Plant Cell Reports, 18, 533-537.
  • Jayasankar, S., Bondada, B.R., Zhijian, L. and Gray, D.J., 2003. Comparative Anatomy and Morphology of Vitis vinifera (Vitaceae) Somatic Embryos from Solid-and Liquid Culture Derived Proembryogenic Masses. American Journal of Botany, 90 (7), 973-979.
  • Kikkert, J.R., Striem, M.J., Vidal, J.R., Wallace, P.G., Barnard, J. and Reisch, B.I., 2005. Long Term Study of Somatic Embryogenesis from Anthers and Ovaries of 12 Grapevine (Vitis spp.) Genotypes. In Vitro Cellular and Developmental Biology Plant, 41(3), 232-239.
  • Leal, F., Pinto-Carnide, O., Guedes-Pinto, H. and Pais, M.S., 2004. Embryogenesis and Plant Regeneration in Vitis vinifera L. by Anther Culture. ISHS Acta Horticulturae 652, I. International Symposium on Grapevine Growing, Commerce and Research, Lisbon, Portugal.
  • Martinelli, L., Gribaudo, I., Semenzato, M. and Poletti, V., 2003. Ovary as Valuable Explant for Somatic Embryogenesis Induction in Grapes (Vitis spp.). ISHS Acta Horticulturae 603, VIII. International Conference on Grape Genetics and Breeding, 2(111), Kecskemet, Hungary.
  • Matsuta, N. and Hirabayashi, T., 1989. Embryogenic Cell Lines from Somatic Embryos of Grape (Vitis vinifera L.). Plant Cell Reports, 7, 684- 681.
  • Matsumoto, T. and Sakai, A., 2003. cryopreservation of axillary shoot tips of in vitro grown grape (Vitis) by a two step vitrification protocol. Euphytica, 131, 299-304.
  • Mhatre, M. and Salunkhe, C.K., Rao, P.S., 2000. Micropropagation of Vitis vinifera L. Towards an İmproved Protocol. Scientia Horticulturae, 84, 357-363.
  • Morgana, C., Di Lorenzo, R. and Carimi, F., 2004. Somatic Embryogenesis of Vitis vinifera L. (cv. Sugraone) from Stigma and Style Culture. Vitis Journal of Grapevine Research, 43(4), 169-173.
  • Murashıge, T. and Skoog, F., 1962. Arevised Medium for Rapid Growth Bioassays with Tobacco Tissue Cultures. Phsysiol. Plant, 15, 473-479.
  • Nakajima, I. and Matsuta, N., 2003. Somatic Embryogenesis from Filaments of Vitis vinifera L. and Vitis labruscana Bailey. Vitis, 42 (1), 53- 54.
  • Nakano, M., Sakakıbara, T., Watanabe, Y. and Mıı, M., 1997. Establishment of Embryogenic Cultures in Several Cultivars of Vitis vinifera and V. x labruscana. Vitis, 36(3), 141-145.
  • Nitsch, J. and Nitsch, C., 1969. Haploid Plants from Pollen Grains. Science, 163, 85-87.
  • Perrin, M., Martin D. and Joly D., Demangeat, G., This, P., Masson, J.E., 2001. Medium-Dependent Response of Grapevine Somatic Embryogenic Cells. Plant Science, 161, 107-116.
  • Salunkhe, C.K., Rao, P.S. and Mhatre, M., 1997. Induction of Somatic Embryogenesis and Plantlets in Tendrils of Vitis vinifera L. Plant Cell Reports, 17, 65-67.
  • Stamp, J.A., Colby, S.M. and Meredith, C.P., 1990a. Improved Shoot Organogenesis from Leaves of Grape. J. Amer. Soc. Hort. Sci., 115(6), 1038- 1042.
  • Stamp, J.A., Colby, S.M. and Meredith, C.P., 1990b. Direct Shoot Organogenesis and Plant Regeneration from Leaves of Grape (Vitis spp.). Plant Cell, Tissue and Organ Culture, 22, 127- 133.
  • Thomas, P., 2000. Microcutting leaf area, weight and position on the stock shoot influence root vigour, shoot growth and incidence of shoot tip necrosis in grape plantlets in vitro. Plant Cell Tissue and Organ Culture, 61, 189-198.
  • Thomas, P. 2001. Leaf number and position effects on the survival and performance of grape microcuttings in vitro, and the sensitivity of the cut nodal region to the medium. Plant Cell Tissue and Organ Culture, 65, 129-139.
  • Zhu, Y.M., Hoshiro, Y., Nakano, M., Takahashi, E. and Mii, M., 1997. Highyl Efficient System of Plant Regeneration from Protoplasts of Grapevine (Vitis vinifera L. ) Through Somatic Embryogenesis by Using Embryogenic Callus Culture and Activated Charcoal. Plant Science, 123, 151-157.

ASMADA (Vitis vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA

Yıl 2008, Cilt: 21 Sayı: 2, 231 - 240, 01.12.2008

Öz

Bu araştırmada bitkisel materyal olarak Kalecik karası üzüm çeşidine ait in vitro bitkilerden alınan gövde ve yaprak sapı eksplantlarının in vitro rejenerasyonları üzerine eksplant tipi, besin ortamları ile ışıklanma süresinin etkileri incelenmiştir. Eksplantlar, farklı konsantrasyonlarda BAP, 2,4-D, zeatin, IBA ve kazein hidrolizat içeren MS ve NN ortamlarına dikildikten sonra aydınlık ve karanlık koşullarda kültüre alınmışlardır. Araştırma sonucunda kallus oluşturan eksplant oranı, direkt ve indirekt adventif sürgün oluşumu, adventif sürgünlerin bitkiye dönüşüm oranı eksplant tipine, besin ortamlarına ve ışıklanma süresine göre değişmiştir. Buna göre gövde eksplantları direkt ve indirekt adventif sürgün oluşumu bakımından en iyi sonuçları verirken; ortamlar içinde kallus oluşumu en fazla 0,2 mg/l BAP+1 mg/l 2,4-D+2,5 mg/l IBA içeren MS ve yalnızca 2 mg/l 2,4-D içeren NN ortamlarından, direkt adventif sürgün gelişimi de 2 mg/l zeatin içeren MS ortamı ile 2 mg/l BAP içeren NN ortamında, indirekt adventif sürgün gelişimi ise 0,2 mg/l BAP+1 mg/l 2,4-D+1 g/l kazein hidrolizat katkılı MS ortamı ile 2 mg/l zeatin NN ortamından elde edildiği belirlenmiştir. Karanlık kültür koşulları kallus oluşumu üzerinde olumlu etkiler de bulunurken; 16/8 sa ışıklanma ise direkt-indirekt adventif sürgün gelişimini artırdığı tespit edilmiştir

Kaynakça

  • Bayır A., Uzun, H. İ. ve Elidemir, A.Y., 2004. Effects of 2,4-D, Kinetin and BAP Combinations on Callus Formation and Organogenesis in Vitis. The 14th FESPB Congress August 23-27, 2004, Cracow/Poland. Acta Physiologiae Plantarum, 26(3), 255.
  • Carimi, F., Barizza, E., Gardiman, M. and Schiavo, F.L., 2005. Somatic Embryogenesis from Stigmas and Styles of Grapevine. In Vitro Cellular and Developmental Biology Plant, 41(3), 249-252.
  • Cheng, Z.M. and Reisch, B.I., 1989. Shoot Regeneration from Petioles and Leaves of Vitis labruscana Catawba. Plant Cell Reports, 8(7), 403-406.
  • Clog, E., Bass, P. and Walter, B., 1990. Plant Regeneration by Organogenesis in Vitis Rootstock Species. Plant Cell Reports, 8(12), 726-728.
  • Goebel Tourand, I., Mauro, M.C., Sossountzov, L., Miginiac, E. and Deloire, A., 1993. Arrest of Somatic Embryo Development in Grapevine: Histological Characterization and The Effect of ABA, BAP and Zeatin in Stimulating Plantlet Development. Plant Cell, Tissue and Organ Culture, 33, 91-103.
  • Emershad, R.L. and Ramming, D.W., 1994. Somatic Embryogenesis and Plant
  • Development from Immature Zygotic Embryos of Seedless Grapes (Vitis vinifera L.). Plant Cell Reports, 14(1), 6-12.
  • Göktürk Baydar, N., 1997. Bağcılıkta In Vitro Mikroaşılama Tekniği ile Çoğaltma Üzerinde Araştırmalar. Ankara Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, 99 s., Ankara.
  • Göktürk Baydar, N., 2000. Asmada (Vitis spp.) Yapraklardan Adventif Sürgün Oluşumu Üzerine Bir Araştırma. Turk J. Biol., 24, 645-656.
  • Göktürk Baydar, N. ve Çetin, S., 2003. Asma Yaprak Eksplantlarının In Vitro Gelişmeleri Üzerine Genotip, Eksplant Tipi ve Besin Ortamlarının Etkileri. 13. Biyoteknoloji Kongresi Bildiriler Kitabı, 99-104, Çanakkale.
  • Gök Tangolar, S. 2002. Asmalarda Somatik Embriyogenesis ve Organogenesis Yoluyla Bitki Elde Edilmesi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, 165 s., Adana.
  • Jayasankar, S., Gray, D.J. and Litz R.Z., 1999. High Efficiency Somatic Embryogenesis and Plant Regeneration from Suspension Cultures of Grapevine. Plant Cell Reports, 18, 533-537.
  • Jayasankar, S., Bondada, B.R., Zhijian, L. and Gray, D.J., 2003. Comparative Anatomy and Morphology of Vitis vinifera (Vitaceae) Somatic Embryos from Solid-and Liquid Culture Derived Proembryogenic Masses. American Journal of Botany, 90 (7), 973-979.
  • Kikkert, J.R., Striem, M.J., Vidal, J.R., Wallace, P.G., Barnard, J. and Reisch, B.I., 2005. Long Term Study of Somatic Embryogenesis from Anthers and Ovaries of 12 Grapevine (Vitis spp.) Genotypes. In Vitro Cellular and Developmental Biology Plant, 41(3), 232-239.
  • Leal, F., Pinto-Carnide, O., Guedes-Pinto, H. and Pais, M.S., 2004. Embryogenesis and Plant Regeneration in Vitis vinifera L. by Anther Culture. ISHS Acta Horticulturae 652, I. International Symposium on Grapevine Growing, Commerce and Research, Lisbon, Portugal.
  • Martinelli, L., Gribaudo, I., Semenzato, M. and Poletti, V., 2003. Ovary as Valuable Explant for Somatic Embryogenesis Induction in Grapes (Vitis spp.). ISHS Acta Horticulturae 603, VIII. International Conference on Grape Genetics and Breeding, 2(111), Kecskemet, Hungary.
  • Matsuta, N. and Hirabayashi, T., 1989. Embryogenic Cell Lines from Somatic Embryos of Grape (Vitis vinifera L.). Plant Cell Reports, 7, 684- 681.
  • Matsumoto, T. and Sakai, A., 2003. cryopreservation of axillary shoot tips of in vitro grown grape (Vitis) by a two step vitrification protocol. Euphytica, 131, 299-304.
  • Mhatre, M. and Salunkhe, C.K., Rao, P.S., 2000. Micropropagation of Vitis vinifera L. Towards an İmproved Protocol. Scientia Horticulturae, 84, 357-363.
  • Morgana, C., Di Lorenzo, R. and Carimi, F., 2004. Somatic Embryogenesis of Vitis vinifera L. (cv. Sugraone) from Stigma and Style Culture. Vitis Journal of Grapevine Research, 43(4), 169-173.
  • Murashıge, T. and Skoog, F., 1962. Arevised Medium for Rapid Growth Bioassays with Tobacco Tissue Cultures. Phsysiol. Plant, 15, 473-479.
  • Nakajima, I. and Matsuta, N., 2003. Somatic Embryogenesis from Filaments of Vitis vinifera L. and Vitis labruscana Bailey. Vitis, 42 (1), 53- 54.
  • Nakano, M., Sakakıbara, T., Watanabe, Y. and Mıı, M., 1997. Establishment of Embryogenic Cultures in Several Cultivars of Vitis vinifera and V. x labruscana. Vitis, 36(3), 141-145.
  • Nitsch, J. and Nitsch, C., 1969. Haploid Plants from Pollen Grains. Science, 163, 85-87.
  • Perrin, M., Martin D. and Joly D., Demangeat, G., This, P., Masson, J.E., 2001. Medium-Dependent Response of Grapevine Somatic Embryogenic Cells. Plant Science, 161, 107-116.
  • Salunkhe, C.K., Rao, P.S. and Mhatre, M., 1997. Induction of Somatic Embryogenesis and Plantlets in Tendrils of Vitis vinifera L. Plant Cell Reports, 17, 65-67.
  • Stamp, J.A., Colby, S.M. and Meredith, C.P., 1990a. Improved Shoot Organogenesis from Leaves of Grape. J. Amer. Soc. Hort. Sci., 115(6), 1038- 1042.
  • Stamp, J.A., Colby, S.M. and Meredith, C.P., 1990b. Direct Shoot Organogenesis and Plant Regeneration from Leaves of Grape (Vitis spp.). Plant Cell, Tissue and Organ Culture, 22, 127- 133.
  • Thomas, P., 2000. Microcutting leaf area, weight and position on the stock shoot influence root vigour, shoot growth and incidence of shoot tip necrosis in grape plantlets in vitro. Plant Cell Tissue and Organ Culture, 61, 189-198.
  • Thomas, P. 2001. Leaf number and position effects on the survival and performance of grape microcuttings in vitro, and the sensitivity of the cut nodal region to the medium. Plant Cell Tissue and Organ Culture, 65, 129-139.
  • Zhu, Y.M., Hoshiro, Y., Nakano, M., Takahashi, E. and Mii, M., 1997. Highyl Efficient System of Plant Regeneration from Protoplasts of Grapevine (Vitis vinifera L. ) Through Somatic Embryogenesis by Using Embryogenic Callus Culture and Activated Charcoal. Plant Science, 123, 151-157.
Toplam 31 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Ziraat Mühendisliği
Bölüm Makaleler
Yazarlar

Z. Babalık Bu kişi benim

N. Göktürk Baydar Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2008
Yayımlandığı Sayı Yıl 2008 Cilt: 21 Sayı: 2

Kaynak Göster

APA Babalık, Z., & Baydar, N. G. (2008). ASMADA (Vitis vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA. Akdeniz University Journal of the Faculty of Agriculture, 21(2), 231-240.
AMA Babalık Z, Baydar NG. ASMADA (Vitis vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA. Akdeniz University Journal of the Faculty of Agriculture. Aralık 2008;21(2):231-240.
Chicago Babalık, Z., ve N. Göktürk Baydar. “ASMADA (Vitis Vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA”. Akdeniz University Journal of the Faculty of Agriculture 21, sy. 2 (Aralık 2008): 231-40.
EndNote Babalık Z, Baydar NG (01 Aralık 2008) ASMADA (Vitis vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA. Akdeniz University Journal of the Faculty of Agriculture 21 2 231–240.
IEEE Z. Babalık ve N. G. Baydar, “ASMADA (Vitis vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA”, Akdeniz University Journal of the Faculty of Agriculture, c. 21, sy. 2, ss. 231–240, 2008.
ISNAD Babalık, Z. - Baydar, N. Göktürk. “ASMADA (Vitis Vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA”. Akdeniz University Journal of the Faculty of Agriculture 21/2 (Aralık 2008), 231-240.
JAMA Babalık Z, Baydar NG. ASMADA (Vitis vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA. Akdeniz University Journal of the Faculty of Agriculture. 2008;21:231–240.
MLA Babalık, Z. ve N. Göktürk Baydar. “ASMADA (Vitis Vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA”. Akdeniz University Journal of the Faculty of Agriculture, c. 21, sy. 2, 2008, ss. 231-40.
Vancouver Babalık Z, Baydar NG. ASMADA (Vitis vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA. Akdeniz University Journal of the Faculty of Agriculture. 2008;21(2):231-40.