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Transient Gus Expression Studies in Onion (Allium cepa L.) and Garlic (Allium sativum L.)

Yıl 2005, Cilt: 18 Sayı: 3, 301 - 304, 01.12.2005

Öz

Three genotypes of onion B-780, N-2-4-1, Hisar 2 and garlic HG-1, G-41 and G-1 were used to study the transient gus expression. Also different explants in onion, callus, shoot tip and roots and in garlic, roots, basal portion and young etiolated leaves along with two plasmids were used to study the effect of different plasmids (pTOK 233 and pCAMBIA 1301) and suitability of explants towards transformation. Results revealed that in onion callus obtained from different explant sources performed significantly superior than shoot tip whereas in root tip no gus expression was observed. In garlic, only basal portion showed transient expression whereas leaves and roots did not show any response. There were no significant differences among genotypes but plasmids showed significant differences. Genotypes differences were not significant; however, the two plasmids showed variables response transient gus assays. Plasmid pCAMBIA 1301 show significant superiority over pTOK 233 in these experiments.

Kaynakça

  • Aldemita, R.R., and Hodges, T. K., 1996. Agrobacterium tumefaciens- mediated transformation of japonica and indica rice varieties. Planta., 199 : 612-617.
  • Anonymous., 2001. National Horticultural Research and development Foundation, Nasik, Maharashtra. NHRDF Newsletter.
  • Barandiaran, X., Pietro, A., di., Martin, J., and Pietro, A. di., 1998. Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L. Plant Cell Rep. 17, 737-741.
  • Bhojwani, S.S., 1980. In vitro propagation of garlic by shoot proliferation. Scientia Hort.. 13, 47-52.
  • Eady, C.C., 1995. Towards the transformation of onion (Allium cepa). N.Z.J. Crop Hort. Sci. 23, 239-250.
  • Eady, C.C., and Lister, C.E., 1998. A comparison of four selective agents for use with Allium cepa L. immature embryos and immature embryo derived cultures. Plant Cell Rep. 18, 117-121.
  • .Eady, C.C., Lister, C.C., Suo, Y., Schaper, D., and Suo, Y.Y., 1996. Transient expression of uida constructs in in vitro onion (Allium cepa L.) cultures following particle bombardment and Agrobacterium mediated DNA-delivery. Plant Cell Rep. 15, 958-962.
  • Eady, C.C., Weld, R.J., and Lister, C.E., 2000. Agrobacterium tumefaciens –mediated transformation and transgenic- plant regeneration of onion (Allium cepa L.). Plant Cell Rep. 19, 376-381.
  • Hiei, Y., Ohta, S., Komari, T., and Kumashiro, T., 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the TDNA. Plant J. 6, 271-282.
  • Jefferson, R.A., Kavanagh, T.A., and Bevan, M.W., 1987. GUS Fusion : ß-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6, 3901-3907.
  • Klein, T.H., Wolf, E.D., Wu, R., and Sanford, J.C., 1987. High velocity microprojectiles for delivering nucleic acids into living cells. Nature. 327, 70-73.
  • Kondo, T., Hasegawa, H., and Suzuki, M., 2000. Transformation and regeneration in garlic (Allium sativum L.) by Agrobacterium mediated gene transfer. Plant Cell Rep. 19, 989-993.
  • Koul, S., Sambyal, M., and Grewal, S., 1994. Embryogenesis and plantlet formation in garlic Allium sativum L. J. Spices and Aromatic Crops. 3, 43-47.
  • Zheng, S.J., Khrustaleva, L., Henken, L., Sofiari, E., Jacobsen, E., Kik, C., and Krens, F.A., 2001. Agrobacterium tumefaciens mediated transformation of Allium cepa L.: the production of transgenic onions and shallots. Mol. Breed., 7, 101-115.

Soğan (Allium cepa L.) ve Sarmısakda (Allium sativum L.) Transient Gus Expression Çalışmaları

Yıl 2005, Cilt: 18 Sayı: 3, 301 - 304, 01.12.2005

Öz

Bu çalışmada, transient gus ekpressiyonu amacıyla, 3 soğan (B-780, N-2-4-1 ve Hisar-2) ve 2 sarmısak (HG1, G-41 and G-1) genotipi kullanılmıştır. Bu amaçla, soğanda kallus, sürgün ucu ve kök; sarmısakta ise kök, basal kısım ve genç etiyolleşmiş yapraklar kullanılarak 2 farklı plazmidin (pTOK 233 ve pCAMBIA 1301) transformasyon için uygunluğu araştırılmıştır. Soğanda eksplant kaynağı olarak kallusun kullanımı sürgün ucuna göre daha başarılı bulunmuş ve kök uçlarında gus epressionu gözlenmemiştir. Sarmısakta, sadece basal kısımda transient ekpression belirlenmiş, yaprak ve kökler her hangi bir yanıt göstermemişlerdir. Genotipler arasında gus transient ekspression bakımından bir farklılık belirlenmemiş, fakat plasmidler arasında farklılık kaydedilmiştir. Plasmid pCAMBIA 1301, pTOK 233’e göre önemli bir üstünlük göstermiştir

Kaynakça

  • Aldemita, R.R., and Hodges, T. K., 1996. Agrobacterium tumefaciens- mediated transformation of japonica and indica rice varieties. Planta., 199 : 612-617.
  • Anonymous., 2001. National Horticultural Research and development Foundation, Nasik, Maharashtra. NHRDF Newsletter.
  • Barandiaran, X., Pietro, A., di., Martin, J., and Pietro, A. di., 1998. Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L. Plant Cell Rep. 17, 737-741.
  • Bhojwani, S.S., 1980. In vitro propagation of garlic by shoot proliferation. Scientia Hort.. 13, 47-52.
  • Eady, C.C., 1995. Towards the transformation of onion (Allium cepa). N.Z.J. Crop Hort. Sci. 23, 239-250.
  • Eady, C.C., and Lister, C.E., 1998. A comparison of four selective agents for use with Allium cepa L. immature embryos and immature embryo derived cultures. Plant Cell Rep. 18, 117-121.
  • .Eady, C.C., Lister, C.C., Suo, Y., Schaper, D., and Suo, Y.Y., 1996. Transient expression of uida constructs in in vitro onion (Allium cepa L.) cultures following particle bombardment and Agrobacterium mediated DNA-delivery. Plant Cell Rep. 15, 958-962.
  • Eady, C.C., Weld, R.J., and Lister, C.E., 2000. Agrobacterium tumefaciens –mediated transformation and transgenic- plant regeneration of onion (Allium cepa L.). Plant Cell Rep. 19, 376-381.
  • Hiei, Y., Ohta, S., Komari, T., and Kumashiro, T., 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the TDNA. Plant J. 6, 271-282.
  • Jefferson, R.A., Kavanagh, T.A., and Bevan, M.W., 1987. GUS Fusion : ß-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6, 3901-3907.
  • Klein, T.H., Wolf, E.D., Wu, R., and Sanford, J.C., 1987. High velocity microprojectiles for delivering nucleic acids into living cells. Nature. 327, 70-73.
  • Kondo, T., Hasegawa, H., and Suzuki, M., 2000. Transformation and regeneration in garlic (Allium sativum L.) by Agrobacterium mediated gene transfer. Plant Cell Rep. 19, 989-993.
  • Koul, S., Sambyal, M., and Grewal, S., 1994. Embryogenesis and plantlet formation in garlic Allium sativum L. J. Spices and Aromatic Crops. 3, 43-47.
  • Zheng, S.J., Khrustaleva, L., Henken, L., Sofiari, E., Jacobsen, E., Kik, C., and Krens, F.A., 2001. Agrobacterium tumefaciens mediated transformation of Allium cepa L.: the production of transgenic onions and shallots. Mol. Breed., 7, 101-115.
Toplam 14 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Ziraat Mühendisliği
Bölüm Makaleler
Yazarlar

A. Khar Bu kişi benim

R. C. Yadav Bu kişi benim

N. Yadav Bu kişi benim

R. D. Bhutanı Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2005
Yayımlandığı Sayı Yıl 2005 Cilt: 18 Sayı: 3

Kaynak Göster

APA Khar, A., Yadav, R. C., Yadav, N., Bhutanı, R. D. (2005). Soğan (Allium cepa L.) ve Sarmısakda (Allium sativum L.) Transient Gus Expression Çalışmaları. Akdeniz University Journal of the Faculty of Agriculture, 18(3), 301-304.
AMA Khar A, Yadav RC, Yadav N, Bhutanı RD. Soğan (Allium cepa L.) ve Sarmısakda (Allium sativum L.) Transient Gus Expression Çalışmaları. Akdeniz University Journal of the Faculty of Agriculture. Aralık 2005;18(3):301-304.
Chicago Khar, A., R. C. Yadav, N. Yadav, ve R. D. Bhutanı. “Soğan (Allium Cepa L.) Ve Sarmısakda (Allium Sativum L.) Transient Gus Expression Çalışmaları”. Akdeniz University Journal of the Faculty of Agriculture 18, sy. 3 (Aralık 2005): 301-4.
EndNote Khar A, Yadav RC, Yadav N, Bhutanı RD (01 Aralık 2005) Soğan (Allium cepa L.) ve Sarmısakda (Allium sativum L.) Transient Gus Expression Çalışmaları. Akdeniz University Journal of the Faculty of Agriculture 18 3 301–304.
IEEE A. Khar, R. C. Yadav, N. Yadav, ve R. D. Bhutanı, “Soğan (Allium cepa L.) ve Sarmısakda (Allium sativum L.) Transient Gus Expression Çalışmaları”, Akdeniz University Journal of the Faculty of Agriculture, c. 18, sy. 3, ss. 301–304, 2005.
ISNAD Khar, A. vd. “Soğan (Allium Cepa L.) Ve Sarmısakda (Allium Sativum L.) Transient Gus Expression Çalışmaları”. Akdeniz University Journal of the Faculty of Agriculture 18/3 (Aralık 2005), 301-304.
JAMA Khar A, Yadav RC, Yadav N, Bhutanı RD. Soğan (Allium cepa L.) ve Sarmısakda (Allium sativum L.) Transient Gus Expression Çalışmaları. Akdeniz University Journal of the Faculty of Agriculture. 2005;18:301–304.
MLA Khar, A. vd. “Soğan (Allium Cepa L.) Ve Sarmısakda (Allium Sativum L.) Transient Gus Expression Çalışmaları”. Akdeniz University Journal of the Faculty of Agriculture, c. 18, sy. 3, 2005, ss. 301-4.
Vancouver Khar A, Yadav RC, Yadav N, Bhutanı RD. Soğan (Allium cepa L.) ve Sarmısakda (Allium sativum L.) Transient Gus Expression Çalışmaları. Akdeniz University Journal of the Faculty of Agriculture. 2005;18(3):301-4.