Moleküler Araştırmalara Uygun Popülasyon Geliştirme

Yıl 2001, Cilt: 14 Sayı: 2, 85 - 90, 02.12.2001

Bülent Uzun M. İlhan Çağırgan

Öz

Bu çalışmada moleküler genetik araştırmalarda kullanılan çeşitli populasyon tipleri tanıtılarak değerlendirilmiştir. Bu amaçla kullanılan dört farklı populasyondan söz edilebilir. Bunlar; yakın izogenik hatlar, F2 populasyonu, double-haploidler ve rekombinant inbred populasyonlardır. Moleküler genetik araştırmalarda kullanılan yakın izogenik hatlar, rekombinant inbredler ve F2 populasyonları homozigot ebeveynlerin melezlenmesiyle elde edilmektedir. Ancak kendilemenin mümkün olmadığı bazı bitki türlerinde çok nadir olmakla birlikte bu populasyonlar heterozigot bireylerin melezlenmesiyle de elde edilebilir. Double-haploid populasyonlar ise, çoğunlukla anter kültürü kullanılarak tek bir polen tanesinden genomun ikiye katlanması sonucunda elde edilmektedir. Her bir populasyon tipinin zayıf veya güçlü tarafları, bunların moleküler genetik çalışmalarda seçilmelerinde önemli rol oynamaktadır. Bu nedenle, araştırıcı bitki cinsine ve araştırdığı özelliğe ve hatta elindeki mevcut şartlara bağlı olarak uygun bir populasyon belirlemeli, gerekirse birden fazla populasyon kullanarak sonuca gitmelidir.

Anahtar Kelimeler

yakın izogenik hat, F2 populasyonu, rekombinant inbred hat, double-haploid populasyonlar, moleküler marker

Population Development For Molecular Studies

Öz

In this study, several types of plant populations used in molecular genetic research were described and evaluated. There are four different types of populations suited for this objective i.e., near isogenic lines, F2 populations, double-haploid populations and recombinant inbred lines. Near isogenic lines, F2 populations and recombinant inbreds have been derived from crosses between homozygous parents. However, several plant species which are intolerant to inbreeding must rely on crosses between heterozygous individuals. Double-haploids are basically obtained by regenarating plants from single pollen grains, and inducing chromosome doubling. Unique strenghts and/or weaknesses of the each population type play an important role for performing molecular genetic research. Therefore, researcher should select a suitable population based on plant species, trait interested, and facilities available. It is also regarded that more than one population may be used to get targetted result when necessary.

Anahtar Kelimeler

Near isogenic lines, F2 population, recombinant inbred lines, double-haploid populations, molecular marker

Kaynakça

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