Application and Using Possibilities of In Vitro Micrografting Technique to Grapevine

Yıl 1999, Cilt: 12 Sayı: 1, 131 - 142, 01.12.1999

Nilgün Göktürk Baydar

Öz

In this article, the in vitro micrografting technique was descripted and applications to grapevine were submitted in detail. In vitro micrografting is a technique that consists of grafting a shoot- tip meristem of 0.1-0.8 mm on top of the decapitated surface of generative or vegetative rootstocks in steril conditions. The technique is applicated to grapevine following 5 steps (obtaining of rootstocks, obtaining of shoot-tip meristems, grafting procedure, growing of micrografted plant in vitro and acclimatization of micrografted plants). In vitro micrografting is applied to grapevine for some purposes such as recov ery of virus and virus¬like free plants, studies on graft incompatibilty mechanism, morpholocigal and anatomical studies on cell activity of graft union, rapid diagnosis of virus and virus-like diseases and quarantine procedures.

Anahtar Kelimeler

Grapevine, In Vitro, Micrografting, Shoot Tip Meristem, Rootstocks

Asmada In Vitro Mikroaşılama Tekniğinin Uygulanışı ve Kullanım Olanakları

Öz

Bu derlemede, in vitro mikroaşılama tekniğinin tanımı, asmada uygulanışı ve kullanım alanları konusunda detaylı bilgi verilmiştir. Büyüklüğü 0.1-0.8 mm arasında değişen sürgün ucu meristeminin, tepesi vurularak değişik şekillerde kesit açılmış vejetatif ya da generatif anaçlar üzerine steril koşullarda yerleştirilme işlemi olarak tanımlanabilen in vitro mikroaşılama; asmaya, anacın yetiştirilmesi, sürgün ucu meristemlerinin elde edilmesi, aşılama, in vitro’da aşılı bitkilerin bakımı ve mikroaşdı bitkilerin dış koşullara alıştınlması olmak üzere birbirini takip eden 5 aşamada uygulanmaktadır. Asmada in vitro mikroaşılama tekniği, virüs ve benzeri etmenlerden arındırılmış bitki elde etme, aşı uyuşmazlığı mekanizmasının incelenmesi, aşı yerinde meydana gelen hücre aktivitelerinin morfolojik ve anatomik olarak belirlenmesi, indeksleme çalışmalarının kısa sürede sonuçlandırılması ve hastalık etmenlerinin yayılmasını önlemeye yönelik karantina önlemleri çerçev esinde kullanılmaktadır

Anahtar Kelimeler

Asma, In Vitro, Mikroaşılama, Sürgün Ucu Meristemi, Anaç

Kaynakça

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