Identification of Five Allium Species with RAPD Markers

Leyla Açık; Bülent Samancı; Mehmet Yapar; Ayhan Kubar

Araştırma Makalesi

Identification of Five Allium Species with RAPD Markers

Yıl 1997, Cilt: 10 Sayı: 1, 143 - 148, 01.12.1997

Leyla Açık Bülent Samancı Mehmet Yapar Ayhan Kubar

Öz

Random amplified polymorphic DNA (RAPD) analysis was applied to Allium species in order to check the degree of polymorphism within the genus. Five species of Allium, A. isauricum, A. myrianthum, A. curtum, A. ılgazasense and A. kararnanoglu were evaluated for variability using a set 10mer random primers. One of the primer revealed scorable polymorphism between Allium species. Variation in banding profiles between species were observed. These were applied to systematic studies within the genus. Ten band positions were scored. Genetic distances between species were calculated and cluster analysis were used for a dendogram showing phylogenetic relations among species studied.

Anahtar Kelimeler

RAPD-PCR, Allium, Allium Taxonomy, Phylogeny

Kaynakça

1. AÇIK, L., B. SAMANCI, H. DUMAN, F. ÜNAL. Polymorphism and Phlylogentic Relations Among Turkish Species In the genus Stenbergia as Determined RAPD-PCR. Turkish Jouna! of Botany. In press.
2. DAVIES, P.H. In Flora of Turkey and East Agean Islands, vol VIII. pp. 360- 364, 1984.
3. HANELT, P. In Onions and Applied Crops. Botany, Physiology and Genetics. CRC Press Inc. Boca, Raton, Florida pp. 1- 6, 1990.
4. LANDRY, B.S., R.Q. LI, W.Y. CHEUNG, R.L. GARANGER. Phlogeny Analysis of 25 Apple Rootstocks Using RAPD Markers and Tactical Gene Tagging. Theor. Appl. Genet. 89, 847-852, 1994
5. SAMBROOK, J. F.F. FRITCH, T. MANÎATIS. In Molecular Clonning. A laboratory Manual,. Second edition. Cold Spring Harbour. 1989.
6. WELSH, J. C. PETERSON, M. MCCLLELAND. Polymorphism Generated by Arbitrarily Primed PCR in The mouse; Applications to Strain Identification and Genetic Mapping. Nucleic Acids Res. 19, 903-906, 1991.
7. WELSH, J., C. PRETZMEN, D. POSTIC, T. SAINT-GIRONS, G. BATONTON, M, MCCLELLAND. Genomic Fingerprinting by Arbitrarily Primed PCR Resolves Borrelia burgdorferi into Three Distinct Phyletic Groups. Int. Sys. Bacterid, 42, 370-377, 1992.
8. WILKIE, S.E., P.G. ISAAC, AND J. R. SLATER. RAPD Markers for Genetic Analysis in Allium. Theor. Appl. Genet. 86,497-504, 1993.
9. WILLIAMS, J.G., A.R. KUBELIK, K. LLIVAC, J. A. RAFALSKI, S. TINGEY. DNA Polymorphism Amplified by Arbitrary Primers Are Useful as Genetic Markers. Nucleic Acids Research. 22, 6531-6535, 1990.
10. WOLF, K, J.P. RIJN. Rapid Detection of Genetic Variabilty in Chrisanthemun Using Random Primers. Heridity. 71,335-341, 1993.

RAPD İzleri ile 5 Allium Türünün Belirlenmesi

Yıl 1997, Cilt: 10 Sayı: 1, 143 - 148, 01.12.1997

Leyla Açık Bülent Samancı Mehmet Yapar Ayhan Kubar

Öz

RAPD analizi cins içersinde polimorfizmim derecesini kontrol etmek için Allium türlerine uygulanmıştır. Allium 'un beş türü, A. isauricum, A. myrianthum, A. curtum, A. ılgazasense and A. kararnanoglu şansa bağlı primerler kullanılarak vary asyonu belirlemek için değerlendirilmişlerdir. Primerlerden biri Allium türleri arasında sayılabilir polimorfizmi ortaya koymuştur. Band profilinde türler arasındaki varyasyonlar gözlenmiştir. Bunlar cins içersinde sistematik çalışmalara uygulanmıştır. 10 band pozisyonu sayılmıştır. Türler arasındaki genetik uzaklıklar hesaplanmış ve kluster analizi çalışılan türler arasındaki filogenetik ilişkileri gösteren dendogram için kullanılmıştır.

Anahtar Kelimeler

RAPD-PCR, Allium, Soğan Taxonomisi, Filojeni

Kaynakça

1. AÇIK, L., B. SAMANCI, H. DUMAN, F. ÜNAL. Polymorphism and Phlylogentic Relations Among Turkish Species In the genus Stenbergia as Determined RAPD-PCR. Turkish Jouna! of Botany. In press.
2. DAVIES, P.H. In Flora of Turkey and East Agean Islands, vol VIII. pp. 360- 364, 1984.
3. HANELT, P. In Onions and Applied Crops. Botany, Physiology and Genetics. CRC Press Inc. Boca, Raton, Florida pp. 1- 6, 1990.
4. LANDRY, B.S., R.Q. LI, W.Y. CHEUNG, R.L. GARANGER. Phlogeny Analysis of 25 Apple Rootstocks Using RAPD Markers and Tactical Gene Tagging. Theor. Appl. Genet. 89, 847-852, 1994
5. SAMBROOK, J. F.F. FRITCH, T. MANÎATIS. In Molecular Clonning. A laboratory Manual,. Second edition. Cold Spring Harbour. 1989.
6. WELSH, J. C. PETERSON, M. MCCLLELAND. Polymorphism Generated by Arbitrarily Primed PCR in The mouse; Applications to Strain Identification and Genetic Mapping. Nucleic Acids Res. 19, 903-906, 1991.
7. WELSH, J., C. PRETZMEN, D. POSTIC, T. SAINT-GIRONS, G. BATONTON, M, MCCLELLAND. Genomic Fingerprinting by Arbitrarily Primed PCR Resolves Borrelia burgdorferi into Three Distinct Phyletic Groups. Int. Sys. Bacterid, 42, 370-377, 1992.
8. WILKIE, S.E., P.G. ISAAC, AND J. R. SLATER. RAPD Markers for Genetic Analysis in Allium. Theor. Appl. Genet. 86,497-504, 1993.
9. WILLIAMS, J.G., A.R. KUBELIK, K. LLIVAC, J. A. RAFALSKI, S. TINGEY. DNA Polymorphism Amplified by Arbitrary Primers Are Useful as Genetic Markers. Nucleic Acids Research. 22, 6531-6535, 1990.
10. WOLF, K, J.P. RIJN. Rapid Detection of Genetic Variabilty in Chrisanthemun Using Random Primers. Heridity. 71,335-341, 1993.

Toplam 10 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Ziraat Mühendisliği
Bölüm	Makaleler
Yazarlar	Leyla Açık Bu kişi benim Bülent Samancı Bu kişi benim Mehmet Yapar Bu kişi benim Ayhan Kubar Bu kişi benim
Yayımlanma Tarihi	1 Aralık 1997
Yayımlandığı Sayı	Yıl 1997 Cilt: 10 Sayı: 1

Kaynak Göster

APA	Açık, L., Samancı, B., Yapar, M., Kubar, A. (1997). Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture, 10(1), 143-148.
AMA	Açık L, Samancı B, Yapar M, Kubar A. Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture. Aralık 1997;10(1):143-148.
Chicago	Açık, Leyla, Bülent Samancı, Mehmet Yapar, ve Ayhan Kubar. “Identification of Five Allium Species With RAPD Markers”. Akdeniz University Journal of the Faculty of Agriculture 10, sy. 1 (Aralık 1997): 143-48.
EndNote	Açık L, Samancı B, Yapar M, Kubar A (01 Aralık 1997) Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture 10 1 143–148.
IEEE	L. Açık, B. Samancı, M. Yapar, ve A. Kubar, “Identification of Five Allium Species with RAPD Markers”, Akdeniz University Journal of the Faculty of Agriculture, c. 10, sy. 1, ss. 143–148, 1997.
ISNAD	Açık, Leyla vd. “Identification of Five Allium Species With RAPD Markers”. Akdeniz University Journal of the Faculty of Agriculture 10/1 (Aralık 1997), 143-148.
JAMA	Açık L, Samancı B, Yapar M, Kubar A. Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture. 1997;10:143–148.
MLA	Açık, Leyla vd. “Identification of Five Allium Species With RAPD Markers”. Akdeniz University Journal of the Faculty of Agriculture, c. 10, sy. 1, 1997, ss. 143-8.
Vancouver	Açık L, Samancı B, Yapar M, Kubar A. Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture. 1997;10(1):143-8.