The Application of Molecular Markers to Plant Breeding

Yıl 1997, Cilt: 10 Sayı: 1, 300 - 305, 01.12.1997

Bülent Samancı Leyla Açık

Öz

Morphological characters are subject to change due to environment. However, DNA markers are less independent of environmental contiditions. They can be obtained from isozyme, RFLP and PCR tecniques. Data are used for cultivar identification, prediction of hybrid performance, measuring the genetic distance, finding heterotic patterns, selection for agronomic traits and mapping genes in chromosomes. The most widely used method for detection of DNA polymorphisms is RFLP and a recent technique, PCR. However, detection of polymorphisms by using PCR technology is faster and less laborious than by using RFLP technology.

Anahtar Kelimeler

Polymorphisms, Genetic Variability, RFLP, PCR

Moleküler Belirleyicilerin Bitki Islahına Uygulanması

Öz

Morfolojik karakterler çevreden dolayı değişim gösterme eğilimindedirler. Fakat DNA belirleyicileri çevreden daha az bağımsızdırlar. Bunlar isozim, RFLP ve PCR tekniklerinden elde edilebilir. Veriler çeşit belirlenmesinde, hibrid verimliliğinin tahmininde, genetik uzaklığın ölçülmesinde, heterotik grupların bulunmasında, agronomik özelliklerin seçiminde ve genlerin kromozom üzerinde haritalanmasında kullanılırlar. DNA polimorfizmin belirlenmesinde RFLP ve PCR teknikleri kullanılmaktadır. Fakat, polimorfizmin ortaya çıkarılmasında PCR teknolojisi RFLP'ye göre daha hızlı olup daha az işgücüne ihtiyaç duymaktadır.

Anahtar Kelimeler

Polymorfizm, Genetik Varyasyon, RFLP, PCR

Kaynakça

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